7L0R: Neurotensin receptor type 1

Structure of NTS-NTSR1-Gi complex in lipid nanodisc, noncanonical state, without AHD. Determined by electron microscopy at 4.2 Å resolution. Released 6 Jan 2021.

Method: Electron microscopy
Resolution: 4.2 Å
Organisms: Rattus norvegicus, Homo sapiens
Chains: 5
Atoms: 6,968
Mol. weight: 128.56 kDa
Released: 6 Jan 2021

Explore 7L0R in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7L0R contains 28 α-helices and 41 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 10 helices, 10 β-strands

Element	Residues	Length	Sheet
α-helix	8-31	24
β-strand	34-39	6	2
α-helix	46-54	9
β-strand	186-190	5	2
β-strand	195-200	6	2
α-helix	201-203	3
α-helix	208-210	3
α-helix	212-215	4
β-strand	220-226	7	2
α-helix	227-230	4
β-strand	233	1	3
β-strand	241	1	3
α-helix	242-254	13
β-strand	263-269	7	2
α-helix	271-280	10
α-helix	296-307	12
β-strand	320-323	4	2
β-strand	325	1	4
β-strand	327	1	4
α-helix	332-350	19

Chain B: 2 helices, 29 β-strands

Element	Residues	Length	Sheet
α-helix	38-39	2
β-strand	47-51	5	5
β-strand	58-63	6	6
β-strand	69-74	6	6
β-strand	78-83	6	6
β-strand	88-94	7	6
β-strand	100-105	6	7
β-strand	111-116	6	7
β-strand	122-125	4	7
β-strand	135-137	3	7
β-strand	148-151	4	8
β-strand	156-160	5	8
β-strand	166-170	5	8
β-strand	176	1	8
β-strand	180	1	8
α-helix	186	1
β-strand	187-192	6	9
β-strand	198-203	6	9
β-strand	208-212	5	9
β-strand	220-222	3	9
β-strand	229-234	6	10
β-strand	240-245	6	10
β-strand	250-254	5	10
β-strand	260-264	5	10
β-strand	273-278	6	11
β-strand	284-289	6	11
β-strand	294-298	5	11
β-strand	303-308	6	11
β-strand	315-320	6	5
β-strand	327-331	5	5
β-strand	336-339	4	5

Chain C: 15 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	61-90	30
α-helix	100-115	16
α-helix	116-120	5
α-helix	121-124	4
α-helix	125-130	6
α-helix	138-172	35
α-helix	174-180	7
α-helix	183-201	19
α-helix	203-206	4
β-strand	208-212	5	1
β-strand	223-227	5	1
α-helix	231-242	12
α-helix	243-247	5
α-helix	248-269	22
α-helix	301-333	33
α-helix	341-370	30
α-helix	374-384	11

Chain G: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	33-47	15

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Neurotensin receptor type 1	C	protein	336	Rattus norvegicus	P20789 (AlphaFold model)
Neurotensin	D	protein	10	Rattus norvegicus	P20068 (AlphaFold model)
Guanine nucleotide-binding protein G(i) subunit alpha-1	A	protein	354	Homo sapiens	P63096 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	361	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(T) subunit gamma-T1	G	protein	83	Homo sapiens	P63211

Sequence of entity 1 (C), FASTA

>7L0R_1 Neurotensin receptor type 1 (chains C)
GPGSGPNSDLDVNTDIYSKVLVTAIYLALFVVGTVGNSVTLFTLARKKSLQSLQSTVDYY
LGSLALSDLLILLLAMPVELYNFIWVHHPWAFGDAGCRGYYFLRDACTYATALNVVSLSV
ERYLAICHPFKAKTLMSRSRTKKFISAIWLASALLAIPMLFTMGLQNLSGDGTHPGGLVC
TPIVDTATLKVVIQVNTFMSFLFPMLVASILNTVIANKLTVMVHQAAFNMTIEPGRVQAL
RRGVLVLRAVVIAFVVCWLPYHVRRLMFCYISDEQWTTFLFDFYHYFYMLTNALVYVSAA
INPILYNLVSANFRQVFLSTLACLCPGTRELEVLFQ

Sequence of entity 2 (D), FASTA

>7L0R_2 Neurotensin (chains D)
GPGGRRPYIL

Sequence of entity 3 (A), FASTA

>7L0R_3 Guanine nucleotide-binding protein G(i) subunit alpha-1 (chains A)
MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEAG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEM
NRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAA
AYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF

Sequence of entity 4 (B), FASTA

>7L0R_4 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MRGSHHHHHHHHHHLEVLFQGPSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDP
VGRIQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSW
VMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVT
SSGDTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCR
QTFTGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFS
KSGRLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIW
N

Sequence of entity 5 (G), FASTA

>7L0R_5 Guanine nucleotide-binding protein G(T) subunit gamma-T1 (chains G)
MYPYDVPDYAPVINIEDLTEKDKLKMEVDQLKKEVTLERMLVSKCCEEVRDYVEERSGED
PLVKGIPEDKNPFKELKGGCVIS

Primary citation

Cryo-EM structure of an activated GPCR-G protein complex in lipid nanodiscs. Zhang, M., Gui, M., Wang, Z.F. et al. Nat Struct Mol Biol (2021) 28:258-267. DOI 10.1038/s41594-020-00554-6 · PubMed

Other PDB entries of the same protein (UniProt P20789 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6YVR 2.46 Å, Crystal structure of the neurotensin receptor 1 in complex with the peptide full agonist…
8FMZ 2.59 Å, Neurotensin receptor allosterism revealed in complex with a biased allosteric modulator
6Z4V 2.6 Å, Crystal structure of the neurotensin receptor 1 (NTSR1-H4bmx) in complex with NTS8-13
4XES 2.6 Å, Structure of active-like neurotensin receptor
6ZIN 2.64 Å, Crystal structure of the neurotensin receptor 1 in complex with the small molecule…
6Z4S 2.71 Å, Crystal structure of the neurotensin receptor 1 (NTSR1-H4bmx) in complex with the small…
6ZA8 2.72 Å, Crystal structure of the neurotensin receptor 1 in complex with the small-molecule…
4BUO 2.75 Å, High Resolution Structure of Thermostable Agonist-bound Neurotensin Receptor 1 Mutant…
4GRV 2.8 Å, The crystal structure of the neurotensin receptor NTS1 in complex with neurotensin (8-13)
6Z8N 2.8 Å, Crystal structure of the neurotensin receptor 1 in complex with the small-molecule full…
8FN1 2.88 Å, CryoEM structure of Go-coupled NTSR1
8FN0 2.89 Å, CryoEM structure of Go-coupled NTSR1 with a biased allosteric modulator

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