8IMZ: Mouse Piezo1-MDFIC complex

Cryo-EM structure of mouse Piezo1-MDFIC complex (composite map). Determined by electron microscopy at 3.66 Å resolution. Released 9 Aug 2023.

Method
Electron microscopy
Resolution
3.66 Å
Organism
Mus musculus
Chains
6
Atoms
29,409
Mol. weight
955.33 kDa
Released
9 Aug 2023

Explore 8IMZ in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8IMZ contains 180 α-helices and 45 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chains A, B and C: 59 helices, 15 β-strands

ElementResiduesLengthSheet
α-helix785-82339
α-helix827-83913
α-helix843-8453
α-helix846-85712
α-helix859-8679
α-helix925-94925
α-helix974-100532
α-helix1010-102213
α-helix1026-105227
α-helix1074-10796
α-helix1094-111320
α-helix1149-115810
α-helix1162-117514
α-helix1181-119818
α-helix1203-122725
α-helix1228-12314
α-helix1244-12496
α-helix1282-129817
α-helix1300-13023
α-helix1303-136462
α-helix1404-14074
α-helix1511-154535
α-helix1552-15576
α-helix1657-16637
α-helix1665-16684
α-helix1673-168513
α-helix1687-170014
α-helix1705-17073
α-helix1709-17135
α-helix1714-17185
α-helix1727-174822
α-helix1771-17744
α-helix1787-180216
α-helix1958-196710
α-helix1977-199620
α-helix2017-204024
α-helix2043-205917
α-helix2060-20645
α-helix2065-20684
α-helix2077-209923
α-helix2116-212712
α-helix2131-214111
α-helix2149-217426
α-helix2185-221329
β-strand2225-223281
β-strand2237-224371
β-strand2248-225032
α-helix2251-22522
α-helix2253-22619
α-helix2267-22726
α-helix2273-22753
α-helix2278-22803
β-strand2281-228442
β-strand228613
α-helix2291-22944
α-helix2298-231013
β-strand2315-2324101
α-helix2327-23293
β-strand2335-233951
β-strand2342-234431
α-helix2349-235810
β-strand2366-237274
β-strand2375-237842
β-strand238612
α-helix2388-23903
β-strand2399-2408104
β-strand2427-243484
β-strand244413
β-strand2447-245042
α-helix2467-248216
α-helix2501-251515
α-helix2520-253213
α-helix2538-25414
α-helix2544-25463
Chains D, E and F: 1 helix, 0 β-strands
ElementResiduesLengthSheet
α-helix231-24414

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Piezo-type mechanosensitive ion channel component 1A, B, Cprotein2547Mus musculusE2JF22 (AlphaFold model)
MyoD family inhibitor domain-containing proteinD, E, Fprotein247Mus musculusQ8BX65 (AlphaFold model)
Sequence of entity 1 (A, B, C), FASTA
>8IMZ_1 Piezo-type mechanosensitive ion channel component 1 (chains A, B, C)
MEPHVLGAGLYWLLLPCTLLAASLLRFNALSLVYLLFLLLLPWLPGPSRHSIPGHTGRLL
RALLCLSLLFLVAHLAFQICLHTVPHLDQFLGQNGSLWVKVSQHIGVTRLDLKDIFNTTR
LVAPDLGVLLASSLCLGLCGRLTRKAGQSRRTQELQDDDDDDDDDDEDIDAAPAVGLKGA
PALATKRRLWLASRFRVTAHWLLMTSGRTLVIVLLALAGIAHPSAFSSIYLVVFLAICTW
WSCHFPLSPLGFNTLCVMVSCFGAGHLICLYCYQTPFIQDMLPPGNIWARLFGLKNFVDL
PNYSSPNALVLNTKHAWPIYVSPGILLLLYYTATSLLKLHKSCPSELRKETPREDEEHEL
ELDHLEPEPQARDATQGEMPMTTEPDLDNCTVHVLTSQSPVRQRPVRPRLAELKEMSPLH
GLGHLIMDQSYVCALIAMMVWSIMYHSWLTFVLLLWACLIWTVRSRHQLAMLCSPCILLY
GLTLCCLRYVWAMELPELPTTLGPVSLHQLGLEHTRYPCLDLGAMLLYLLTFWLLLRQFV
KEKLLKKQKVPAALLEVTVADTEPTQTQTLLRSLGELVTGIYVKYWIYVCAGMFIVVSFA
GRLVVYKIVYMFLFLLCLTLFQVYYTLWRKLLRVFWWLVVAYTMLVLIAVYTFQFQDFPT
YWRNLTGFTDEQLGDLGLEQFSVSELFSSILIPGFFLLACILQLHYFHRPFMQLTDLEHV
PPPGTRHPRWAHRQDAVSEAPLLEHQEEEEVFREDGQSMDGPHQATQVPEGTASKWGLVA
DRLLDLAASFSAVLTRIQVFVRRLLELHVFKLVALYTVWVALKEVSVMNLLLVVLWAFAL
PYPRFRPMASCLSTVWTCIIIVCKMLYQLKIVNPHEYSSNCTEPFPNNTNLQPLEINQSL
LYRGPVDPANWFGVRKGYPNLGYIQNHLQILLLLVFEAVVYRRQEHYRRQHQQAPLPAQA
VCADGTRQRLDQDLLSCLKYFINFFFYKFGLEICFLMAVNVIGQRMNFMVILHGCWLVAI
LTRRRREAIARLWPNYCLFLTLFLLYQYLLCLGMPPALCIDYPWRWSKAIPMNSALIKWL
YLPDFFRAPNSTNLISDFLLLLCASQQWQVFSAERTEEWQRMAGINTDHLEPLRGEPNPI
PNFIHCRSYLDMLKVAVFRYLFWLVLVVVFVAGATRISIFGLGYLLACFYLLLFGTTLLQ
KDTRAQLVLWDCLILYNVTVIISKNMLSLLSCVFVEQMQSNFCWVIQLFSLVCTVKGYYD
PKEMMTRDRDCLLPVEEAGIIWDSICFFFLLLQRRIFLSHYFLHVSADLKATALQASRGF
ALYNAANLKSINFHRQIEEKSLAQLKRQMKRIRAKQEKYRQSQASRGQLQSKDPQDPSQE
PGPDSPGGSSPPRRQWWRPWLDHATVIHSGDYFLFESDSEEEEEALPEDPRPAAQSAFQM
AYQAWVTNAQTVLRQRRERARQERAEQLASGGDLNPDVEPVDVPEDEMAGRSHMMQRVLS
TMQFLWVLGQATVDGLTRWLRAFTKHHRTMSDVLCAERYLLTQELLRVGEVRRGVLDQLY
VGEDEATLSGPVETRDGPSTASSGLGAEEPLSSMTDDTSSPLSTGYNTRSGSEEIVTDAG
DLQAGTSLHGSQELLANARTRMRTASELLLDRRLHIPELEEAERFEAQQGRTLRLLRAGY
QCVAAHSELLCYFIIILNHMVTASAASLVLPVLVFLWAMLTIPRPSKRFWMTAIVFTEVM
VVTKYLFQFGFFPWNSYVVLRRYENKPYFPPRILGLEKTDSYIKYDLVQLMALFFHRSQL
LCYGLWDHEEDRYPKDHCRSSVKDREAKEEPEAKLESQSETGTGHPKEPVLAGTPRDHIQ
GKGSIRSKDVIQDPPEDLKPRHTRHISIRFRRRKETPGPKGTAVMETEHEEGEGKETTER
KRPRHTQEKSKFRERMKAAGRRLQSFCVSLAQSFYQPLQRFFHDILHTKYRAATDVYALM
FLADIVDIIIIIFGFWAFGKHSAATDIASSLSDDQVPQAFLFMLLVQFGTMVIDRALYLR
KTVLGKLAFQVVLVVAIHIWMFFILPAVTERMFSQNAVAQLWYFVKCIYFALSAYQIRCG
YPTRILGNFLTKKYNHLNLFLFQGFRLVPFLVELRAVMDWVWTDTTLSLSNWMCVEDIYA
NIFIIKCSRETEKKYPQPKGQKKKKIVKYGMGGLIILFLIAIIWFPLLFMSLIRSVVGVV
NQPIDVTVTLKLGGYEPLFTMSAQQPSIVPFTPQAYEELSQQFDPYPLAMQFISQYSPED
IVTAQIEGSSGALWRISPPSRAQMKQELYNGTADITLRFTWNFQRDLAKGGTVEYTNEKH
TLELAPNSTARRQLAQLLEGRPDQSVVIPHLFPKYIRAPNGPEANPVKQLQPDEEEDYLG
VRIQLRREQVGTGASGEQAGTKASDFLEWWVIELQDCKADCNLLPMVIFSDKVSPPSLGF
LAGYGIVGLYVSIVLVVGKFVRGFFSEISHSIMFEELPCVDRILKLCQDIFLVRETRELE
LEEELYAKLIFLYRSPETMIKWTRERE
Sequence of entity 2 (D, E, F), FASTA
>8IMZ_2 MyoD family inhibitor domain-containing protein (chains D, E, F)
MSCAGEALAPGPAEQQCPVEAGGGRLGSPAHEACNEDNTEKDKRPATSGHTRCGLMRDQS
IWPNPSAGELVRTQPERLPQLQTSAQEPGKEETGKIKNGGHTRMSNGNGIPHGAKHVSVE
NHKISAPVSQKMHRKIQSSLSVNNDISKKSKVNAVFSPKAASSPEDCCVHCILACLFCEF
LTLCNIVLGQASCGICTSEACCCCCGDEMGDDCSCPCDMDCGIMDACCESSDCLEICMEC
CGICFPS

Primary citation

MyoD-family inhibitor proteins act as auxiliary subunits of Piezo channels. Zhou, Z., Ma, X., Lin, Y. et al. Science (2023) 381:799-804. DOI 10.1126/science.adh8190 · PubMed

Other PDB entries of the same protein (UniProt E2JF22 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

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