9VED: Mouse Piezo1-MDFI complex

The cryo-EM structure of mouse Piezo1-MDFI complex. Determined by electron microscopy at 3.63 Å resolution. Released 8 Apr 2026.

Method
Electron microscopy
Resolution
3.63 Å
Organisms
Mus musculus, Aequorea victoria
Chains
6
Atoms
29,400
Mol. weight
1049.83 kDa
Released
8 Apr 2026

Explore 9VED in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9VED contains 186 α-helices and 54 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chains A, B and C: 61 helices, 18 β-strands

ElementResiduesLengthSheet
α-helix785-80420
α-helix805-8095
α-helix810-8123
α-helix814-82310
α-helix827-83913
α-helix846-8516
α-helix853-86715
α-helix925-94925
α-helix974-100532
α-helix1011-102212
α-helix1026-104924
α-helix1074-10796
α-helix1094-111320
α-helix1149-115810
α-helix1162-117514
α-helix1181-120020
α-helix1203-122725
α-helix1228-12314
α-helix1244-12496
α-helix1282-129817
α-helix1301-136464
α-helix1404-14074
α-helix1412-14154
α-helix1511-152313
α-helix1525-154521
α-helix1554-15574
α-helix1657-166812
α-helix1673-168513
α-helix1687-170014
α-helix1709-17135
α-helix1714-17185
α-helix1727-174822
α-helix1771-17744
α-helix1787-180216
α-helix1958-196710
α-helix1977-199620
α-helix2017-204024
α-helix2043-205917
α-helix2060-20645
α-helix2065-20684
α-helix2077-209923
α-helix2116-212712
α-helix2131-214111
α-helix2149-217426
α-helix2185-221329
α-helix2216-22183
β-strand221911
β-strand2225-223062
β-strand223213
β-strand2239-224352
β-strand2248-225034
α-helix2251-22522
α-helix2253-22619
α-helix2267-22748
α-helix2278-22803
β-strand2281-228664
α-helix2291-22944
α-helix2298-230912
α-helix23141
β-strand2315-231625
β-strand231713
β-strand2320-232452
α-helix2327-23293
β-strand2335-233952
β-strand2343-234425
α-helix2349-235810
β-strand2366-237276
β-strand2375-237844
β-strand238614
α-helix2388-23903
β-strand2399-2408106
β-strand2427-243486
β-strand2444-245074
β-strand245311
α-helix2467-248216
α-helix2490-24923
α-helix2501-251515
α-helix2520-253213
α-helix2537-25404
Chains D, E and F: 1 helix, 0 β-strands
ElementResiduesLengthSheet
α-helix229-24315

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Piezo-type mechanosensitive ion channel component 1,Green fluorescent proteinA, B, Cprotein2815Mus musculus, Aequorea victoriaE2JF22 (AlphaFold model), P42212 (AlphaFold model)
Isoform I-mfA of MyoD family inhibitorD, E, Fprotein269Mus musculusP70331 (AlphaFold model)
Sequence of entity 1 (A, B, C), FASTA
>9VED_1 Piezo-type mechanosensitive ion channel component 1,Green fluorescent protein (chains A, B, C)
MEPHVLGAGLYWLLLPCTLLAASLLRFNALSLVYLLFLLLLPWLPGPSRHSIPGHTGRLL
RALLCLSLLFLVAHLAFQICLHTVPHLDQFLGQNGSLWVKVSQHIGVTRLDLKDIFNTTR
LVAPDLGVLLASSLCLGLCGRLTRKAGQSRRTQELQDDDDDDDDDDEDIDAAPAVGLKGA
PALATKRRLWLASRFRVTAHWLLMTSGRTLVIVLLALAGIAHPSAFSSIYLVVFLAICTW
WSCHFPLSPLGFNTLCVMVSCFGAGHLICLYCYQTPFIQDMLPPGNIWARLFGLKNFVDL
PNYSSPNALVLNTKHAWPIYVSPGILLLLYYTATSLLKLHKSCPSELRKETPREDEEHEL
ELDHLEPEPQARDATQGEMPMTTEPDLDNCTVHVLTSQSPVRQRPVRPRLAELKEMSPLH
GLGHLIMDQSYVCALIAMMVWSIMYHSWLTFVLLLWACLIWTVRSRHQLAMLCSPCILLY
GLTLCCLRYVWAMELPELPTTLGPVSLHQLGLEHTRYPCLDLGAMLLYLLTFWLLLRQFV
KEKLLKKQKVPAALLEVTVADTEPTQTQTLLRSLGELVTGIYVKYWIYVCAGMFIVVSFA
GRLVVYKIVYMFLFLLCLTLFQVYYTLWRKLLRVFWWLVVAYTMLVLIAVYTFQFQDFPT
YWRNLTGFTDEQLGDLGLEQFSVSELFSSILIPGFFLLACILQLHYFHRPFMQLTDLEHV
PPPGTRHPRWAHRQDAVSEAPLLEHQEEEEVFREDGQSMDGPHQATQVPEGTASKWGLVA
DRLLDLAASFSAVLTRIQVFVRRLLELHVFKLVALYTVWVALKEVSVMNLLLVVLWAFAL
PYPRFRPMASCLSTVWTCIIIVCKMLYQLKIVNPHEYSSNCTEPFPNNTNLQPLEINQSL
LYRGPVDPANWFGVRKGYPNLGYIQNHLQILLLLVFEAVVYRRQEHYRRQHQQAPLPAQA
VCADGTRQRLDQDLLSCLKYFINFFFYKFGLEICFLMAVNVIGQRMNFMVILHGCWLVAI
LTRRRREAIARLWPNYCLFLTLFLLYQYLLCLGMPPALCIDYPWRWSKAIPMNSALIKWL
YLPDFFRAPNSTNLISDFLLLLCASQQWQVFSAERTEEWQRMAGINTDHLEPLRGEPNPI
PNFIHCRSYLDMLKVAVFRYLFWLVLVVVFVAGATRISIFGLGYLLACFYLLLFGTTLLQ
KDTRAQLVLWDCLILYNVTVIISKNMLSLLSCVFVEQMQSNFCWVIQLFSLVCTVKGYYD
PKEMMTRDRDCLLPVEEAGIIWDSICFFFLLLQRRIFLSHYFLHVSADLKATALQASRGF
ALYNAANLKSINFHRQIEEKSLAQLKRQMKRIRAKQEKYRQSQASRGQLQSKDPQDPSQE
PGPDSPGGSSPPRRQWWRPWLDHATVIHSGDYFLFESDSEEEEEALPEDPRPAAQSAFQM
AYQAWVTNAQTVLRQRRERARQERAEQLASGGDLNPDVEPVDVPEDEMAGRSHMMQRVLS
TMQFLWVLGQATVDGLTRWLRAFTKHHRTMSDVLCAERYLLTQELLRVGEVRRGVLDQLY
VGEDEATLSGPVETRDGPSTASSGLGAEEPLSSMTDDTSSPLSTGYNTRSGSEEIVTDAG
DLQAGTSLHGSQELLANARTRMRTASELLLDRRLHIPELEEAERFEAQQGRTLRLLRAGY
QCVAAHSELLCYFIIILNHMVTASAASLVLPVLVFLWAMLTIPRPSKRFWMTAIVFTEVM
VVTKYLFQFGFFPWNSYVVLRRYENKPYFPPRILGLEKTDSYIKYDLVQLMALFFHRSQL
LCYGLWDHEEDRYPKDHCRSSVKDREAKEEPEAKLESQSETGTGHPKEPVLAGTPRDHIQ
GKGSIRSKDVIQDPPEDLKPRHTRHISIRFRRRKETPGPKGTAVMETEHEEGEGKETTER
KRPRHTQEKSKFRERMKAAGRRLQSFCVSLAQSFYQPLQRFFHDILHTKYRAATDVYALM
FLADIVDIIIIIFGFWAFGKHSAATDIASSLSDDQVPQAFLFMLLVQFGTMVIDRALYLR
KTVLGKLAFQVVLVVAIHIWMFFILPAVTERMFSQNAVAQLWYFVKCIYFALSAYQIRCG
YPTRILGNFLTKKYNHLNLFLFQGFRLVPFLVELRAVMDWVWTDTTLSLSNWMCVEDIYA
NIFIIKCSRETEKKYPQPKGQKKKKIVKYGMGGLIILFLIAIIWFPLLFMSLIRSVVGVV
NQPIDVTVTLKLGGYEPLFTMSAQQPSIVPFTPQAYEELSQQFDPYPLAMQFISQYSPED
IVTAQIEGSSGALWRISPPSRAQMKQELYNGTADITLRFTWNFQRDLAKGGTVEYTNEKH
TLELAPNSTARRQLAQLLEGRPDQSVVIPHLFPKYIRAPNGPEANPVKQLQPDEEEDYLG
VRIQLRREQVGTGASGEQAGTKASDFLEWWVIELQDCKADCNLLPMVIFSDKVSPPSLGF
LAGYGIVGLYVSIVLVVGKFVRGFFSEISHSIMFEELPCVDRILKLCQDIFLVRETRELE
LEEELYAKLIFLYRSPETMIKWTRERESNSLEVLFQGPTAAAAVSKGEELFTGVVPILVE
LDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDH
MKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNIL
GHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPD
NHYLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGGGHHHHHHHHHH
Sequence of entity 2 (D, E, F), FASTA
>9VED_2 Isoform I-mfA of MyoD family inhibitor (chains D, E, F)
MDYKDDDDKGGGGSSTTNGSSATMSQVSGQCPSRCDAPHGVPSAALDPAQTMSLLPGLEV
ARSTHPVEASSEEGFPEEAAPSMPHDSGLRAQQALNSIDLDVPTEAVTCQPQGNPQGCTP
LLPNGSSHDHLSEPGSAGHAGNGALGGSKAHRKLQTHPSLGSQAGRKSRGSARSASQVPL
QAQEDCCVHCILSCLFCEFLTLCNILLDCATCGSCSSEDSCLCCCCCGSGECADCDLPCD
LDCGIVDACCESADCLEICMECCGLCFSS

Primary citation

MDFIC2 is a sensory neuron-specific PIEZO channel auxiliary subunit. Zhang, Y., Dai, F. Proc Natl Acad Sci U S A (2026).

Other PDB entries of the same protein (UniProt E2JF22 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

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