8UIS: Transcription complex Pol II-DSIF-NELF-TFIIS

Structure of transcription complex Pol II-DSIF-NELF-TFIIS. Determined by electron microscopy at 3.23 Å resolution. Released 20 Mar 2024.

Method: Electron microscopy
Resolution: 3.23 Å
Organisms: synthetic construct, Homo sapiens, Sus scrofa
Chains: 19
Atoms: 40,233
Mol. weight: 834.09 kDa
Ligands: MG, ZN
Released: 20 Mar 2024

Explore 8UIS in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8UIS contains 243 α-helices and 243 β-strands across 16 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 76 helices, 63 β-strands

Element	Residues	Length	Sheet
β-strand	15	1	9
β-strand	20-22	3	10
α-helix	28-34	7
β-strand	47	1	11
β-strand	52	1	11
α-helix	53	1
β-strand	86-89	4	12
α-helix	97-99	3
α-helix	100-108	9
β-strand	110	1	13
β-strand	117	1	13
α-helix	124-132	9
α-helix	137-149	13
β-strand	190-194	5	14
β-strand	197-201	5	14
β-strand	214-216	3	14
α-helix	218-226	9
α-helix	230-235	6
α-helix	245-248	4
β-strand	252-255	4	12
β-strand	264-266	3	15
β-strand	270-272	3	15
α-helix	273-274	2
α-helix	275-295	21
α-helix	300-318	19
α-helix	326-328	3
α-helix	340-342	3
β-strand	358-359	2	16
β-strand	362-366	5	17
β-strand	367-369	3	18
β-strand	377-380	4	17
α-helix	382-387	6
β-strand	389-393	5	19
α-helix	399-408	10
β-strand	416-420	5	19
β-strand	426-428	3	19
α-helix	435-438	4
β-strand	445-449	5	19
α-helix	450	1
β-strand	455-459	5	17
α-helix	463	1
α-helix	466-468	3
β-strand	469-476	8	17
β-strand	482-484	3	18
α-helix	489-492	4
β-strand	500-504	5	17
α-helix	509-514	6
α-helix	515-519	5
β-strand	520	1	17
α-helix	521-524	4
β-strand	526-527	2	20
β-strand	532-533	2	20
α-helix	539-548	10
β-strand	554-555	2	21
α-helix	557-563	7
α-helix	564-566	3
α-helix	573-574	2
β-strand	575	1	22
α-helix	576-577	2
β-strand	579-581	3	23
β-strand	585	1	23
β-strand	586-587	2	21
α-helix	588-595	8
β-strand	602-605	4	24
α-helix	611-613	3
β-strand	627-631	5	24
β-strand	634-637	4	24
α-helix	642-645	4
α-helix	652-683	32
α-helix	696-721	26
α-helix	725-728	4
α-helix	733-759	27
α-helix	765-772	8
α-helix	778-785	8
β-strand	789-790	2	25
β-strand	793	1	26
β-strand	796	1	26
α-helix	797-799	3
β-strand	822-823	2	25
α-helix	833-855	23
α-helix	859-867	9
β-strand	872-873	2	27
β-strand	879-880	2	27
β-strand	886-888	3	27
α-helix	891-893	3
β-strand	896	1	28
β-strand	902-905	4	29
α-helix	913-920	8
α-helix	927-933	7
β-strand	934	1	30
α-helix	936-944	9
α-helix	946-969	24
β-strand	976-979	4	29
α-helix	983-994	12
β-strand	1002	1	30
α-helix	1006-1019	14
α-helix	1028-1037	10
α-helix	1039-1048	10
α-helix	1051-1053	3
α-helix	1054-1058	5
α-helix	1062-1079	18
β-strand	1080	1	28
α-helix	1081-1082	2
α-helix	1087-1096	10
α-helix	1098-1101	4
α-helix	1102-1104	3
α-helix	1120-1128	9
β-strand	1139-1142	4	31
α-helix	1146-1148	3
α-helix	1151-1159	9
β-strand	1164-1165	2	32
α-helix	1166-1169	4
β-strand	1170-1176	7	33
α-helix	1187-1196	10
β-strand	1211-1216	6	33
α-helix	1218-1223	6
α-helix	1228-1239	12
β-strand	1243-1247	5	33
β-strand	1255-1261	7	33
α-helix	1283-1294	12
β-strand	1297-1299	3	32
β-strand	1308-1310	3	31
β-strand	1319-1322	4	34
β-strand	1328-1331	4	34
β-strand	1335-1337	3	31
α-helix	1343-1348	6
β-strand	1358-1359	2	31
α-helix	1363-1368	6
α-helix	1371-1387	17
α-helix	1395-1405	11
α-helix	1416-1419	4
α-helix	1426-1432	7
α-helix	1435-1445	11
β-strand	1448-1449	2	10
α-helix	1454-1459	6
α-helix	1462-1464	3
α-helix	1467-1469	3
β-strand	1472-1475	4	35
α-helix	1477-1481	5

Chain B: 61 helices, 78 β-strands

Element	Residues	Length	Sheet
α-helix	19-37	19
α-helix	41-49	9
α-helix	50-54	5
α-helix	55-61	7
α-helix	63-65	3
β-strand	66-68	3	36
β-strand	82-93	12	36
β-strand	97-99	3	37
β-strand	105-107	3	37
α-helix	110-116	7
β-strand	121-134	14	36
β-strand	140-153	14	36
α-helix	168-173	6
β-strand	185-187	3	38
β-strand	190-194	5	38
α-helix	195	1
β-strand	196-200	5	39
α-helix	201	1
β-strand	206-208	3	40
β-strand	215-223	9	40
β-strand	233-240	8	40
α-helix	241	1
α-helix	249-252	4
β-strand	256-259	4	40
β-strand	263	1	41
β-strand	267-268	2	40
α-helix	269-275	7
α-helix	281-288	8
α-helix	295-300	6
α-helix	302-307	6
α-helix	314-323	10
β-strand	325	1	41
α-helix	332-341	10
α-helix	342-346	5
α-helix	349-351	3
α-helix	355-357	3
α-helix	358-376	19
α-helix	381-384	4
α-helix	388-390	3
β-strand	391-394	4	39
α-helix	396-424	29
α-helix	428-430	3
α-helix	431-434	4
α-helix	438-450	13
β-strand	453	1	42
α-helix	459-461	3
β-strand	463	1	42
β-strand	467-469	3	38
α-helix	475-482	8
β-strand	484-486	3	39
α-helix	490-493	4
α-helix	503-505	3
β-strand	509	1	43
β-strand	523-525	3	39
β-strand	526	1	43
β-strand	527	1	44
β-strand	531-534	4	45
α-helix	539-547	9
β-strand	550-552	3	46
α-helix	553-555	3
α-helix	558-563	6
β-strand	565-569	5	46
β-strand	572-577	6	46
α-helix	580-593	14
α-helix	598	1
β-strand	602-606	5	46
β-strand	611-615	5	46
β-strand	618-627	10	45
β-strand	628-629	2	47
β-strand	632-633	2	47
α-helix	637-644	8
α-helix	653-658	6
β-strand	662-666	5	45
α-helix	667-670	4
β-strand	675-676	2	45
β-strand	695-696	2	45
α-helix	700-703	4
β-strand	705	1	44
α-helix	714-716	3
α-helix	719-728	10
α-helix	729-731	3
β-strand	732	1	48
α-helix	738-741	4
β-strand	747-751	5	49
β-strand	755	1	50
β-strand	759-760	2	51
α-helix	762-765	4
β-strand	776-782	7	52
β-strand	793-797	5	52
α-helix	798-802	5
β-strand	808-817	10	49
β-strand	828-829	2	53
β-strand	837	1	53
β-strand	848	1	54
β-strand	853	1	55
β-strand	854	1	54
α-helix	855	1
β-strand	859	1	49
β-strand	865-867	3	55
β-strand	869-872	4	53
α-helix	873	1
β-strand	889-891	3	53
β-strand	894-895	2	55
β-strand	903-911	9	49
β-strand	917-927	11	49
α-helix	928-929	2
β-strand	934-936	3	52
β-strand	943-949	7	52
β-strand	956	1	56
α-helix	961-963	3
β-strand	965-967	3	52
α-helix	971-975	5
α-helix	978-993	16
α-helix	996	1
β-strand	997-998	2	51
α-helix	1008-1016	9
β-strand	1025-1026	2	57
β-strand	1029	1	56
α-helix	1035	1
β-strand	1036	1	56
α-helix	1037	1
β-strand	1041-1042	2	57
β-strand	1043-1050	8	52
β-strand	1051	1	48
α-helix	1055-1058	4
β-strand	1060-1062	3	17
β-strand	1067	1	58
β-strand	1074	1	58
β-strand	1084-1085	2	16
α-helix	1088-1097	10
α-helix	1100-1104	5
α-helix	1105-1109	5
β-strand	1113-1119	7	9
β-strand	1125	1	9
β-strand	1129	1	59
β-strand	1134	1	59
β-strand	1146-1152	7	9
α-helix	1153-1164	12
β-strand	1171-1172	2	10

Chain C: 10 helices, 12 β-strands

Element	Residues	Length	Sheet
β-strand	8-14	7	60
β-strand	18-24	7	60
α-helix	28-40	13
β-strand	43-55	13	61
α-helix	61-69	9
β-strand	73	1	62
α-helix	78-81	4
α-helix	82-84	3
β-strand	98-107	10	61
β-strand	113-116	4	63
α-helix	117-119	3
β-strand	121-122	2	61
β-strand	129	1	62
β-strand	149-153	5	63
β-strand	157-169	13	61
α-helix	174-176	3
β-strand	179-185	7	60
α-helix	200-202	3
α-helix	204-206	3
α-helix	218-220	3
β-strand	229-235	7	60
α-helix	241-268	28

Chain D: 8 helices, 1 β-strand

Element	Residues	Length	Sheet
α-helix	24-26	3
β-strand	30-32	3	35
α-helix	34-49	16
α-helix	55-58	4
α-helix	59-70	12
α-helix	77-89	13
α-helix	94-103	10
α-helix	108-114	7
α-helix	124-137	14

Chain E: 13 helices, 11 β-strands

Element	Residues	Length	Sheet
α-helix	3-23	21
β-strand	26-27	2	64
α-helix	30-33	4
α-helix	37-43	7
β-strand	60-64	5	64
β-strand	71-75	5	64
β-strand	81-82	2	65
α-helix	84-97	14
β-strand	101-106	6	64
β-strand	109-110	2	65
α-helix	112-121	10
β-strand	126-131	6	64
α-helix	134-136	3
α-helix	139-141	3
β-strand	147-150	4	66
α-helix	151-152	2
α-helix	153-163	11
α-helix	167-169	3
α-helix	171	1
β-strand	172-173	2	66
α-helix	178-183	6
α-helix	185-186	2
β-strand	190-197	8	66
β-strand	201-209	9	66

Chain F: 5 helices, 4 β-strands

Element	Residues	Length	Sheet
β-strand	56	1	67
α-helix	57-58	2
α-helix	59-74	16
α-helix	77-79	3
α-helix	89-99	11
β-strand	104-109	6	35
β-strand	115-119	5	35
β-strand	124	1	67
α-helix	125	1

Chain G: 4 helices, 14 β-strands

Element	Residues	Length	Sheet
β-strand	2-13	12	35
α-helix	15-17	3
α-helix	22-34	13
β-strand	38-39	2	35
β-strand	43-54	12	35
α-helix	55-56	2
β-strand	58-59	2	35
α-helix	60	1
β-strand	66-77	12	35
β-strand	84-92	9	68
β-strand	96-101	6	68
β-strand	104-109	6	68
β-strand	118-119	2	69
β-strand	128-129	2	69
β-strand	136-137	2	69
β-strand	142-153	12	68
β-strand	156-162	7	68
β-strand	169-170	2	68

Chain H: 3 helices, 9 β-strands

Element	Residues	Length	Sheet
β-strand	4-14	11	23
β-strand	25-32	8	23
β-strand	38-44	7	23
β-strand	55-61	7	23
β-strand	74	1	22
α-helix	82-83	2
α-helix	84-87	4
β-strand	90-101	12	23
β-strand	111-118	8	23
β-strand	121-128	8	23
α-helix	129-132	4
β-strand	140-148	9	23

8 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Non-template DNA	N	DNA	50	synthetic construct
RNA	P	RNA	22	synthetic construct
Template DNA	T	DNA	50	synthetic construct
Negative elongation factor C/D	W	protein	590	Homo sapiens	Q8IXH7 (AlphaFold model)
Transcription elongation factor SPT5	Z	protein	1087	Homo sapiens	O00267 (AlphaFold model)
DNA-directed RNA polymerase subunit	A	protein	1970	Sus scrofa	A0A8D1DPV6
DNA-directed RNA polymerase subunit beta	B	protein	1174	Sus scrofa	I3LGP4 (AlphaFold model)
DNA-directed RNA polymerase II subunit RPB3	C	protein	271	Sus scrofa	I3LCH3
RNA polymerase Rpb4/RPC9 core domain-containing protein	D	protein	142	Sus scrofa	A0A4X1VM56
DNA-directed RNA polymerase II subunit E	E	protein	210	Sus scrofa	I3LSI7
DNA-directed RNA polymerases I, II, and III subunit RPABC2	F	protein	127	Sus scrofa	A0A4X1VEK9
DNA-directed RNA polymerase II subunit RPB7	G	protein	172	Sus scrofa	A0A4X1VKG7

7 more molecules are not listed.

Sequence of entity 1 (N), FASTA

>8UIS_1 Non-template DNA (chains N)
GGTCAGTACGTCCGGCATAGTTGCGCCCGTAAATTCAGATCTTCCAGTGG

Sequence of entity 2 (P), FASTA

>8UIS_2 RNA (chains P)
UUUUUUGGCAUAGUUGCGCCCG

Sequence of entity 3 (T), FASTA

>8UIS_3 Template DNA (chains T)
CCACTGGAAGATCTGAATTTACGGGCGCAACTATGCCGGACGTACTGACC

Sequence of entity 4 (W), FASTA

>8UIS_4 Negative elongation factor C/D (chains W)
MAGAVPGAIMDEDYYGSAAEWGDEADGGQQEDDSGEGEDDAEVQQECLHKFSTRDYIMEP
SIFNTLKRYFQAGGSPENVIQLLSENYTAVAQTVNLLAEWLIQTGVEPVQVQETVENHLK
SLLIKHFDPRKADSIFTEEGETPAWLEQMIAHTTWRDLFYKLAEAHPDCLMLNFTVKLIS
DAGYQGEITSVSTACQQLEVFSRVLRTSLATILDGGEENLEKNLPEFAKMVCHGEHTYLF
AQAMMSVLAQEEQGGSAVRRIAQEVQRFAQEKGHDASQITLALGTAASYPRACQALGAML
SKGALNPADITVLFKMFTSMDPPPVELIRVPAFLDLFMQSLFKPGARINQDHKHKYIHIL
AYAASVVETWKKNKRVSINKDELKSTSKAVETVHNLCCNENKGASELVAELSTLYQCIRF
PVVAMGVLKWVDWTVSEPRYFQLQTDHTPVHLALLDEISTCHQLLHPQVLQLLVKLFETE
HSQLDVMEQLELKKTLLDRMVHLLSRGYVLPVVSYIRKCLEKLDTDISLIRYFVTEVLDV
IAPPYTSDFVQLFLPILENDSIAGTIKTEGEHDPVTEFIAHCKSNFIMVN

Sequence of entity 5 (Z), FASTA

>8UIS_5 Transcription elongation factor SPT5 (chains Z)
MSDSEDSNFSEEEDSERSSDGEEAEVDEERRSAAGSEKEEEPEDEEEEEEEEEYDEEEEE
EDDDRPPKKPRHGGFILDEADVDDEYEDEDQWEDGAEDILEKEEIEASNIDNVVLDEDRS
GARRLQNLWRDQREEELGEYYMKKYAKSSVGETVYGGSDELSDDITQQQLLPGVKDPNLW
TVKCKIGEERATAISLMRKFIAYQFTDTPLQIKSVVAPEHVKGYIYVEAYKQTHVKQAIE
GVGNLRLGYWNQQMVPIKEMTDVLKVVKEVANLKPKSWVRLKRGIYKDDIAQVDYVEPSQ
NTISLKMIPRIDYDRIKARMSLKDWFAKRKKFKRPPQRLFDAEKIRSLGGDVASDGDFLI
FEGNRYSRKGFLFKSFAMSAVITEGVKPTLSELEKFEDQPEGIDLEVVTESTGKEREHNF
QPGDNVEVCEGELINLQGKILSVDGNKITIMPKHEDLKDMLEFPAQELRKYFKMGDHVKV
IAGRFEGDTGLIVRVEENFVILFSDLTMHELKVLPRDLQLCSETASGVDVGGQHEWGELV
QLDPQTVGVIVRLERETFQVLNMYGKVVTVRHQAVTRKKDNRFAVALDSEQNNIHVKDIV
KVIDGPHSGREGEIRHLFRSFAFLHCKKLVENGGMFVCKTRHLVLAGGSKPRDVTNFTVG
GFAPMSPRISSPMHPSAGGQRGGFGSPGGGSGGMSRGRGRRDNELIGQTVRISQGPYKGY
IGVVKDATESTARVELHSTCQTISVDRQRLTTVGSRRPGGMTSTYGRTPMYGSQTPMYGS
GSRTPMYGSQTPLQDGSRTPHYGSQTPLHDGSRTPAQSGAWDPNNPNTPSRAEEEYEYAF
DDEPTPSPQAYGGTPNPQTPGYPDPSSPQVNPQYNPQTPGTPAMYNTDQFSPYAAPSPQG
SYQPSPSPQSYHQVAPSPAGYQNTHSPASYHPTPSPMAYQASPSPSPVGYSPMTPGAPSP
GGYNPHTPGSGIEQNSSDWVTTDIQVKVRDTYLDTQVVGQTGVIRSVTGGMCSVYLKDSE
KVVSISSEHLEPITPTKNNKVKVILGEDREATGVLLSIDGEDGIVRMDLDEQLKILNLRF
LGKLLEA

Sequence of entity 6 (A), FASTA

>8UIS_6 DNA-directed RNA polymerase subunit (chains A)
MHGGGPPSGDSACPLRTIKRVQFGVLSPDELKRMSVTEGGIKYPETTEGGRPKLGGLMDP
RQGVIERTGRCQTCAGNMTECPGHFGHIELAKPVFHVGFLVKTMKVLRCVCFFCSKLLVD
SNNPKIKDILAKSKGQPKKRLTHVYDLCKGKNICEGGEEMDNKFGVEQPEGDEDLTKEKG
HGGCGRYQPRIRRSGLELYAEWKHVNEDSQEKKILLSPERVHEIFKRISDEECFVLGMEP
RYARPEWMIVTVLPVPPLSVRPAVVMQGSARNQDDLTHKLADIVKINNQLRRNEQNGAAA
HVIAEDVKLLQFHVATMVDNELPGLPRAMQKSGRPLKSLKQRLKGKEGRVRGNLMGKRVD
FSARTVITPDPNLSIDQVGVPRSIAANMTFAEIVTPFNIDRLQELVRRGNSQYPGAKYII
RDNGDRIDLRFHPKPSDLHLQTGYKVERHMCDGDIVIFNRQPTLHKMSMMGHRVRILPWS
TFRLNLSVTTPYNADFDGDEMNLHLPQSLETRAEIQELAMVPRMIVTPQSNRPVMGIVQD
TLTAVRKFTKRDVFLERGEVMNLLMFLSTWDGKVPQPAILKPRPLWTGKQIFSLIIPGHI
NCIRTHSTHPDDEDSGPYKHISPGDTKVVVENGELIMGILCKKSLGTSAGSLVHISYLEM
GHDITRLFYSNIQTVINNWLLIEGHTIGIGDSIADSKTYQDIQNTIKKAKQDVIEVIEKA
HNNELEPTPGNTLRQTFENQVNRILNDARDKTGSSAQKSLSEYNNFKSMVVSGAKGSKIN
ISQVIAVVGQQNVEGKRIPFGFKHRTLPHFIKDDYGPESRGFVENSYLAGLTPTEFFFHA
MGGREGLIDTAVKTAETGYIQRRLIKSMESVMVKYDATVRNSINQVVQLRYGEDGLAGES
VEFQNLATLKPSNKAFEKKFRFDYTNERALRRTLQEDLVKDVLSNAHIQNELEREFERMR
EDREVLRVIFPTGDSKVVLPCNLLRMIWNAQKIFHINPRLPSDLHPIKVVEGVKELSKKL
VIVNGDDPLSRQAQENATLLFNIHLRSTLCSRRMAEEFRLSGEAFDWLLGEIESKFNQAI
AHPGEMVGALAAQSLGEPATQMTLNTFHYAGVSAKNVTLGVPRLKELINISKKPKTPSLT
VFLLGQSARDAERAKDILCRLEHTTLRKVTANTAIYYDPNPQSTVVAEDQEWVNVYYEMP
DFDVARISPWLLRVELDRKHMTDRKLTMEQIAEKINAGFGDDLNCIFNDDNAEKLVLRIR
IMNSDENKMQEEEEVVDKMDDDVFLRCIESNMLTDMTLQGIEQISKVYMHLPQTDNKKKI
IITEDGEFKALQEWILETDGVSLMRVLSEKDVDPVRTTSNDIVEIFTVLGIEAVRKALER
ELYHVISFDGSYVNYRHLALLCDTMTCRGHLMAITRHGVNRQDTGPLMKCSFEETVDVLM
EAAAHGESDPMKGVSENIMLGQLAPAGTGCFDLLLDAEKCKYGMEIPTNIPGLGAAGPTG
MFFGSAPSPMGGISPAMTPWNQGATPAYGAWSPSVGSGMTPGAAGFSPSAASDASGFSPG
YSPAWSPTPGSPGSPGPSSPYIPSPGGAMSPSYSPTSPAYEPRSPGGYTPQSPSYSPTSP
SYSPTSPSYSPTSPNYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSP
TSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPS
YSPTSPNYSPTSPNYTPTSPSYSPTSPSYSPTSPNYTPTSPNYSPTSPSYSPTSPSYSPT
SPSYSPSSPRYTPQSPTYTPSSPSYSPSSPSYSPTSPKYTPTSPSYSPSSPEYTPTSPKY
SPTSPKYSPTSPKYSPTSPTYSPTTPKYSPTSPTYSPTSPVYTPTSPKYSPTSPTYSPTS
PKYSPTSPTYSPTSPKGSTYSPTSPGYSPTSPTYSLTSPAISPDDSDEEN

Sequence of entity 7 (B), FASTA

>8UIS_7 DNA-directed RNA polymerase subunit beta (chains B)
MYDADEDMQYDEDDDEITPDLWQEACWIVISSYFDEKGLVRQQLDSFDEFIQMSVQRIVE
DAPPIDLQAEAQHASGEVEEPPRYLLKFEQIYLSKPTHWERDGAPSPMMPNEARLRNLTY
SAPLYVDITKTVIKEGEEQLQTQHQKTFIGKIPIMLRSTYCLLNGLTDRDLCELNECPLD
PGGYFIINGSEKVLIAQEKMATNTVYVFAKKDSKYAYTGECRSCLENSSRPTSTIWVSML
ARGGQGAKKSAIGQRIVATLPYIKQEVPIIIVFRALGFVSDRDILEHIIYDFEDPEMMEM
VKPSLDEAFVIQEQNVALNFIGSRGAKPGVTKEKRIKYAKEVLQKEMLPHVGVSDFCETK
KAYFLGYMVHRLLLAALGRRELDDRDHYGNKRLDLAGPLLAFLFRGMFKNLLKEVRIYAQ
KFIDRGKDFNLELAIKTRIISDGLKYSLATGNWGDQKKAHQARAGVSQVLNRLTFASTLS
HLRRLNSPIGRDGKLAKPRQLHNTLWGMVCPAETPEGHAVGLVKNLALMAYISVGSQPSP
ILEFLEEWSMENLEEISPAAIADATKIFVNGCWVGIHKDPEQLMNTLRKLRRQMDIIVSE
VSMIRDIREREIRIYTDAGRICRPLLIVEKQKLLLKKRHIDQLKEREYNNYSWQDLVASG
VVEYIDTLEEETVMLAMTPDDLQEKEVAYCSTYTHCEIHPSMILGVCASIIPFPDHNQSP
RNTYQSAMGKQAMGVYITNFHVRMDTLAHVLYYPQKPLVTTRSMEYLRFRELPAGINSIV
AIASYTGYNQEDSVIMNRSAVDRGFFRSVFYRSYKEQESKKGFDQEEVFEKPTRETCQGM
RHAIYDKLDDDGLIAPGVRVSGDDVIIGKTVTLPENEDELEGTNRRYTKRDCSTFLRTSE
TGIVDQVMVTLNQEGYKFCKIRVRSVRIPQIGDKFASRHGQKGTCGIQYRQEDMPFTCEG
ITPDIIINPHAIPSRMTIGHLIECLQGKVSANKGEIGDATPFNDAVNVQKISNLLSDYGY
HLRGNEVLYNGFTGRKITSQIFIGPTYYQRLKHMVDDKIHSRARGPIQILNRQPMEGRSR
DGGLRFGEMERDCQIAHGAAQFLRERLFEASDPYQVHVCNLCGIMAIANTRTHTYECRGC
RNKTQISLVRMPYACKLLFQELMSMSIAPRMMSV

Sequence of entity 8 (C), FASTA

>8UIS_8 DNA-directed RNA polymerase II subunit RPB3 (chains C)
MPYANQPTVRITELTDENVKFIIENTDLAVANSIRRVFIAEVPIIAIDWVQIDANSSVLH
DEFIAHRLGLIPLTSDDIVDKLQYSRDCTCEEFCPECSVEFTLDVRCNEDQTRHVTSRDL
ISNSPRVIPVTSRNRDNDPSDYVEQDDILIVKLRKGQELRLRAYAKKGFGKEHAKWNPTA
GVAFEYDPDNALRHTVYPKPEEWPKSEYSELDEDESQAPYDPNGKPERFYYNVESCGSLR
PETIVLSALSGLKKKLSDLQTQLSHEIQSDV

Sequence of entity 9 (D), FASTA

>8UIS_9 RNA polymerase Rpb4/RPC9 core domain-containing protein (chains D)
MAAGGSDPRAGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQNESAEDEQELSEV
FMKTLNYTARFSRFKNRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSLEG
RFEDEELQQILDDIQTKRSFQY

Sequence of entity 10 (E), FASTA

>8UIS_10 DNA-directed RNA polymerase II subunit E (chains E)
MDDEEETYRLWKIRKTIMQLCHDRGYLVTQDELDQTLEEFKAQFGDKPSEGRPRRTDLTV
LVAHNDDPTDQMFVFFPEEPKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVD
MAPKYILEQFLQQELLINITEHELVPEHVVMTKEEVTELLARYKLRENQLPRIQAGDPVA
RYFGIKRGQVVKIIRPSETAGRYITYRLVQ

Sequence of entity 11 (F), FASTA

>8UIS_11 DNA-directed RNA polymerases I, II, and III subunit RPABC2 (chains F)
MSDNEDNFDGDDFDDVEEDEGLDDLENAEEEGQENVEILPSGERPQANQKRITTPYMTKY
ERARVLGTRALQIAMCAPVMVELEGETDPLLIAMKELKARKIPIIIRRYLPDGSYEDWGV
DELIISD

Sequence of entity 12 (G), FASTA

>8UIS_12 DNA-directed RNA polymerase II subunit RPB7 (chains G)
MFYHISLEHEILLHPRYFGPNLLNTVKQKLFTEVEGTCTGKYGFVIAVTTIDNIGAGVIQ
PGRGFVLYPVKYKAIVFRPFKGEVVDAVVTQVNKVGLFTEIGPMSCFISRHSIPSEMEFD
PNSNPPCYKTMDEDIVIQQDDEIRLKIVGTRVDKNDIFAIGSLMDDYLGLVS

Ligands and cofactors

ID	Name	Formula	Copies
MG	Magnesium ion	Mg	1
ZN	Zinc ion	Zn	8

Primary citation

Distinct negative elongation factor conformations regulate RNA polymerase II promoter-proximal pausing. Su, B.G., Vos, S.M. Mol Cell (2024) 84:1243. DOI 10.1016/j.molcel.2024.01.023 · PubMed

Other PDB entries of the same protein (UniProt Q8IXH7 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8UHG 2.7 Å, Structure of paused transcription complex Pol II-DSIF-NELF - poised post-translocated
8UI0 2.7 Å, Structure of poised transcription complex Pol II-DSIF-NELF - pre-translocated
8JJ6 2.72 Å, Structure of the NELF-BCE complex
5L3X 2.75 Å, Crystal structure of negative elongation factor subcomplex NELF-AC
8UHD 2.8 Å, Structure of paused transcription complex Pol II-DSIF-NELF - post-translocated
6GML 3.2 Å, Structure of paused transcription complex Pol II-DSIF-NELF
9J0O 3.3 Å, Arrested elongation complex of mammalian RNA polymerase II with nucleosome (AEC1-nuc)
9J0P 3.3 Å, Arrested elongation complex of mammalian RNA polymerase II with nucleosome (AEC2-nuc)
9J0N 3.4 Å, Paused elongation complex of mammalian RNA polymerase II with nucleosome (PEC2-nuc)
8UHA 3.5 Å, Structure of paused transcription complex Pol II-DSIF-NELF - tilted
7PKS 3.6 Å, Structural basis of Integrator-mediated transcription regulation
8W8E 3.9 Å, human co-transcriptional RNA capping enzyme RNGTT

Browse structure collections

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