8XWV: CXCR2

Structure of CXCR2 bound to CXCL1 (CXCR2-CXCL1-Go Full map). Determined by electron microscopy at 3.07 Å resolution. Released 22 Jan 2025.

Method: Electron microscopy
Resolution: 3.07 Å
Organisms: Homo sapiens, Mus musculus
Chains: 7
Atoms: 9,211
Mol. weight: 161.51 kDa
Released: 22 Jan 2025

Explore 8XWV in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8XWV contains 43 α-helices and 79 β-strands across 7 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 10 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	9-30	22
β-strand	33-40	8	7
α-helix	46-50	5
β-strand	186-191	6	7
β-strand	196-201	6	7
α-helix	209-211	3
α-helix	213-216	4
β-strand	221-227	7	7
α-helix	246-255	10
α-helix	258-260	3
α-helix	263-264	2
β-strand	265-270	6	7
α-helix	272-281	10
α-helix	297-309	13
β-strand	319-323	5	7
α-helix	331-350	20

Chain B: 4 helices, 30 β-strands

Element	Residues	Length	Sheet
α-helix	4-24	21
α-helix	30-34	5
β-strand	47-51	5	8
β-strand	58-63	6	9
β-strand	69-74	6	9
β-strand	78-83	6	9
β-strand	89-94	6	9
α-helix	95	1
β-strand	100-105	6	10
β-strand	111-116	6	10
β-strand	121-125	5	10
β-strand	134-139	6	10
β-strand	146-151	6	11
β-strand	157-161	5	11
β-strand	165-168	4	11
β-strand	178-181	4	11
β-strand	187	1	12
β-strand	191-192	2	13
β-strand	198-202	5	13
β-strand	203	1	12
β-strand	208-212	5	13
β-strand	220-222	3	13
β-strand	229-234	6	14
β-strand	240-245	6	14
β-strand	250-254	5	14
β-strand	260-264	5	14
α-helix	272	1
β-strand	273-278	6	15
β-strand	284-289	6	15
β-strand	294-298	5	15
β-strand	303-307	5	15
β-strand	315-320	6	8
β-strand	327-331	5	8
β-strand	336-339	4	8

Chain D: 2 helices, 7 β-strands

Element	Residues	Length	Sheet
β-strand	2	1	1
β-strand	15	1	2
α-helix	20-22	3
β-strand	23-29	7	2
β-strand	32	1	3
β-strand	35	1	3
β-strand	39-44	6	2
β-strand	49-52	4	2
α-helix	57-66	10

Chain E: 2 helices, 6 β-strands

Element	Residues	Length	Sheet
β-strand	15	1	2
α-helix	20-22	3
β-strand	23-28	6	2
β-strand	32	1	4
β-strand	35	1	4
β-strand	40-44	5	2
β-strand	49-52	4	2
α-helix	57-67	11

Chain G: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	8-23	16
α-helix	30-43	14
α-helix	45-47	3
α-helix	53-55	3

Chain H: 5 helices, 25 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	16
β-strand	11-12	2	17
β-strand	17-25	9	16
α-helix	29-31	3
β-strand	34-39	6	18
β-strand	45-51	7	18
α-helix	53-55	3
β-strand	58-60	3	18
β-strand	68-71	4	16
β-strand	78-84	7	16
β-strand	92-98	7	18
β-strand	110-111	2	18
β-strand	115-117	3	18
β-strand	118-119	2	17
α-helix	137-139	3
β-strand	140-141	2	19
β-strand	146-149	4	20
β-strand	155-161	7	19
β-strand	166	1	21
β-strand	172	1	21
β-strand	174-179	6	20
β-strand	186-190	5	20
β-strand	194-195	2	20
β-strand	203-208	6	19
β-strand	211-216	6	19
α-helix	221-223	3
β-strand	225-231	7	20
α-helix	237	1
β-strand	239	1	20
β-strand	243-247	5	20

Chain R: 16 helices, 5 β-strands

Element	Residues	Length	Sheet
α-helix	45-75	31
α-helix	82-98	17
α-helix	101-110	10
α-helix	116-149	34
α-helix	154-160	7
α-helix	162-176	15
α-helix	178-182	5
β-strand	184	1	5
β-strand	187	1	6
β-strand	192	1	1
β-strand	195	1	6
β-strand	198	1	5
α-helix	204-211	8
α-helix	213-216	4
α-helix	217-221	5
α-helix	222-240	19
α-helix	246-278	33
α-helix	286-303	18
α-helix	306-310	5
α-helix	311-315	5
α-helix	319-329	11

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Growth-regulated alpha protein	D, E	protein	73	Homo sapiens	P09341 (AlphaFold model)
C-X-C chemokine receptor type 2	R	protein	416	Homo sapiens	P25025 (AlphaFold model)
Guanine nucleotide-binding protein G(o) subunit alpha	A	protein	240	Homo sapiens	P09471 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	350	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	56	Homo sapiens	P59768
Antibody fragment ScFv16	H	protein	256	Mus musculus

Sequence of entity 1 (D, E), FASTA

>8XWV_1 Growth-regulated alpha protein (chains D, E)
ASVATELRCQCLQTLQGIHPKNIQSVNVKSPGPHCAQTEVIATLKNGRKACLNPASPIVK
KIIEKMLNSDKSN

Sequence of entity 2 (R), FASTA

>8XWV_2 C-X-C chemokine receptor type 2 (chains R)
MGKTIIALSYIFCLVFADYKDDDDAANFTPVNGSSGNQSVRLVTSSSLEVLFQGPGSEDF
NMESDSFEDFWKGEDLSNYSYSSTLPPFLLDAAPCEPESLEINKYFVVIIYALVFLLSLL
GNSLVMLVILYSRVGRSVTDVYLLNLALADLLFALTLPIWAASKVNGWIFGTFLCKVVSL
LKEVNFYSGILLLACISVDRYLAIVHATRTLTQKRYLVKFICLSIWGLSLLLALPVLLFR
RTVYSSNVSPACYEDMGNNTANWRMLLRILPQSFGFIVPLLIMLFCYGFTLRTLFKAHMG
QKHRAMRVIFAVVLIFLLCWLPYNLVLLADTLMRTQVIQETCERRNHIDRALDATEILGI
LHSCLNPLIYAFIGQKFRHGLLKILAIHGLISKDSLPKDSRPSFVGSSSGHTSTTL

Sequence of entity 3 (A), FASTA

>8XWV_3 Guanine nucleotide-binding protein G(o) subunit alpha (chains A)
MGHHHHHHENLYFQGTLSAEERAALERSKAIEKNLKEDGISAAKDVKLLLLGADNSGKST
IVKQMKIIHGGSGGSGGTTGIVETHFTFKNLHFRLFDVGGQRSERKKWIHCFEDVTAIIF
CVDLSDYNRMHESLMLFDSICNNKFFIDTSIILFLNKKDLFGEKIKKSPLTICFPEYTGP
NTYEDAAAYIQAQFESKNRSPNKEIYCHMTCATDTNNAQVIFDAVTDIIIANNLRGCGLY

Sequence of entity 4 (B), FASTA

>8XWV_4 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRT
LRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGN
YVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWD
IETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDIN
AICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYD
DFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 5 (G), FASTA

>8XWV_5 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
ASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENPFR

Sequence of entity 6 (H), FASTA

>8XWV_6 Antibody fragment ScFv16 (chains H)
DVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYY
ADTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVS
SGGGGSGGGGSGGGGSDIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLELKGSLEVLFQ

Primary citation

Molecular basis of promiscuous chemokine binding and structural mimicry at the C-X-C chemokine receptor, CXCR2. Saha, S., Sano, F.K., Sharma, S. et al. Mol Cell (2025) 85:976-988.e9. DOI 10.1016/j.molcel.2025.01.024 · PubMed

Other PDB entries of the same protein (UniProt P09341 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9EJC 2.98 Å, Cryo-EM Structure of CXCL1-KSHV ORF74-Gi-scFv16 Complex
8K4O 3.01 Å, Cryo-EM structure of Kaposi's Sarcoma-Associated Herpesvirus-G Protein-Coupled Receptor…
8XWA 3.48 Å, Structure of CXCR2 bound to CXCL1 (Ligand-receptor focused map)
1MGS The solution structure of melanoma growth stimulating activity
1MSG Solution structure of gro(slash)melanoma growth stimulatory activity determined by 1H…
1MSH Solution structure of gro(slash)melanoma growth stimulatory activity determined by 1H…
1ROD Chimeric protein of interleukin 8 and human melanoma growth stimulating activity…

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