9BQ3: Human Amylin2 Receptor

Human Amylin2 Receptor in Complex with Gs and Cagrilintide. Determined by electron microscopy at 2.8 Å resolution. Released 23 Apr 2025.

Method: Electron microscopy
Resolution: 2.8 Å
Organisms: Homo sapiens, Lama glama
Chains: 7
Atoms: 10,035
Mol. weight: 183.3 kDa
Ligands: A1B90
Released: 23 Apr 2025

Explore 9BQ3 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9BQ3 contains 49 α-helices and 60 β-strands across 7 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 9 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	13-38	26
β-strand	40-47	8	7
α-helix	53-58	6
β-strand	208-214	7	7
β-strand	217-223	7	7
α-helix	234-238	5
β-strand	243-249	7	7
α-helix	265-277	13
β-strand	286-292	7	7
α-helix	294-303	10
α-helix	313-316	4
α-helix	326-327	2
α-helix	332-350	19
β-strand	359-363	5	7
α-helix	371-390	20

Chain B: 4 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	3-25	23
α-helix	30-33	4
α-helix	34-36	3
α-helix	38-39	2
β-strand	47-51	5	8
β-strand	58-63	6	9
β-strand	69-74	6	9
β-strand	78-83	6	9
β-strand	89-94	6	9
β-strand	100-105	6	10
β-strand	111-116	6	10
β-strand	121-125	5	10
β-strand	134-139	6	10
β-strand	146-151	6	11
β-strand	156-161	6	11
β-strand	166-170	5	11
β-strand	175-180	6	11
β-strand	187-192	6	12
β-strand	198-203	6	12
β-strand	207-212	6	12
β-strand	218-223	6	12
β-strand	229-234	6	13
β-strand	240-245	6	13
β-strand	250-254	5	13
β-strand	260-264	5	13
β-strand	273-278	6	14
β-strand	284-289	6	14
β-strand	294-298	5	14
β-strand	304-308	5	14
β-strand	315-320	6	8
β-strand	327-331	5	8
β-strand	336-339	4	8

Chain E: 7 helices, 0 β-strands

Element	Residues	Length
α-helix	61-76	16
α-helix	90-106	17
α-helix	110	1
α-helix	114-126	13
α-helix	134-136	3
α-helix	140-142	3
α-helix	143-167	25

Chain G: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	9-23	15
α-helix	30-43	14

Chain N: 3 helices, 12 β-strands

Element	Residues	Length	Sheet
β-strand	3-8	6	15
β-strand	11-12	2	16
β-strand	17-25	9	15
α-helix	29-31	3
β-strand	34-39	6	17
β-strand	45-51	7	17
β-strand	58-60	3	17
α-helix	62-64	3
β-strand	68-73	6	15
β-strand	78-84	7	15
α-helix	88-90	3
β-strand	92-98	7	17
β-strand	118	1	17
β-strand	122-124	3	17
β-strand	125-126	2	16

Chain P: 3 helices, 1 β-strand

Element	Residues	Length	Sheet
β-strand	4	1	1
α-helix	5-7	3
α-helix	8-19	12
α-helix	28-31	4

Chain R: 21 helices, 13 β-strands

Element	Residues	Length	Sheet
α-helix	40-59	20
α-helix	71	1
β-strand	72	1	2
α-helix	73-74	2
β-strand	75-76	2	3
β-strand	81-82	2	3
β-strand	85	1	2
β-strand	89-94	6	4
β-strand	107-112	6	4
β-strand	118	1	4
β-strand	120	1	5
β-strand	127	1	5
β-strand	130	1	4
α-helix	132-135	4
α-helix	138-172	35
α-helix	179-200	22
α-helix	201-205	5
α-helix	210-214	5
α-helix	217-245	29
β-strand	246	1	6
β-strand	250	1	6
α-helix	260-263	4
α-helix	264-268	5
α-helix	271-284	14
α-helix	288-290	3
α-helix	296-298	3
α-helix	299-330	32
α-helix	334-352	19
α-helix	355-358	4
β-strand	361	1	1
α-helix	366-381	16
α-helix	383-388	6
α-helix	389-393	5
α-helix	396-405	10

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Receptor activity-modifying protein 2	E	protein	156	Homo sapiens	O60895 (AlphaFold model)
Cagrilintide	P	protein	39	Homo sapiens	P10997 (AlphaFold model)
Calcitonin receptor	R	protein	462	Homo sapiens	P30988 (AlphaFold model)
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	A	protein	394	Homo sapiens	P63092 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	350	Homo sapiens	P62873
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768
Nanobody 35	N	protein	138	Lama glama

Sequence of entity 1 (E), FASTA

>9BQ3_1 Receptor activity-modifying protein 2 (chains E)
MKTIIALSYIFCLVFADYKDDDDKPLPTTGTPGSEGGTVKNYETAVQFCWNHYKDQMDPI
EKDWCDWAMISRPYSTLRDCLEHFAELFDLGFPNPLAERIIFETHQIHFANCSLVQPTFS
DPPEDVLLAMIIAPICLIPFLITLVVWRSKDSEAQA

Sequence of entity 2 (P), FASTA

>9BQ3_2 Cagrilintide (chains P)
EKCNTATCATQRLAEFLRHSSNNFGPILPPTNVGSNTPX

Sequence of entity 3 (R), FASTA

>9BQ3_3 Calcitonin receptor (chains R)
GPAAFSNQTYPTIEPKPFLYVVGRKKMMDAQYKCYDRMQQLPAYQGEGPYCNRTWDGWLC
WDDTPAGVLSYQFCPDYFPDFDPSEKVTKYCDEKGVWFKHPENNRTWSNYTMCNAFTPEK
LKNAYVLYYLAIVGHSLSIFTLVISLGIFVFFRSLGCQRVTLHKNMFLTYILNSMIIIIH
LVEVVPNGELVRRDPVSCKILHFFHQYMMACNYFWMLCEGIYLHTLIVVAVFTEKQRLRW
YYLLGWGFPLVPTTIHAITRAVYFNDNCWLSVETHLLYIIHGPVMAALVVNFFFLLNIVR
VLVTKMRETHEAESHMYLKAVKATMILVPLLGIQFVVFPWRPSNKMLGKIYDYVMHSLIH
FQGFFVATIYCFCNNEVQTTVKRQWAQFKIQWNQRWGRRPSNRSARAAAAAAEAGDIPIY
ICHQELRNEPANNQGEESAEIIPLNIIEQESSAPAGLEVLFQ

Sequence of entity 4 (A), FASTA

>9BQ3_4 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKNTIVKQM
RILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELA
NPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLD
KIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFND
VTAIIFVVASSSYNMVIREDNQTNRLQAALKLFDSIWNNKWLRDTSVILFLNKQDLLAEK
VLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCY
PHFTCSVDTENIRRVFNDCRDIIQRMHLRQYELL

Sequence of entity 5 (B), FASTA

>9BQ3_5 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRT
LRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGN
YVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWD
IETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDIN
AICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYD
DFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 6 (G), FASTA

>9BQ3_6 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 7 (N), FASTA

>9BQ3_7 Nanobody 35 (chains N)
QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISY
TGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQ
GTQVTVSSHHHHHHEPEA

Ligands and cofactors

ID	Name	Formula	Copies
A1B90	icosanedioic acid	C20 H38 O4	1

Primary citation

Structural and dynamic features of cagrilintide binding to calcitonin and amylin receptors. Cao, J., Belousoff, M.J., Johnson, R.M. et al. Nat Commun (2025) 16:3389-3389. DOI 10.1038/s41467-025-58680-y · PubMed

Other PDB entries of the same protein (UniProt O60895 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4RWF 1.76 Å, Crystal structure of the CLR:RAMP2 extracellular domain heterodimer with bound…
6V2E 1.83 Å, Crystal structure of the human CLR:RAMP2 extracellular domain heterodimer with bound…
3AQE 2.0 Å, Crystal structure of the extracellular domain of human RAMP2
2XVT 2.05 Å, Structure of the extracellular domain of human RAMP2
7TYX 2.55 Å, Human Amylin2 Receptor in complex with Gs and rat amylin peptide
3AQF 2.6 Å, Crystal structure of the human CRLR/RAMP2 extracellular complex
6UUN 3.0 Å, CryoEM Structure of the active Adrenomedullin 1 receptor G protein complex with…
7TYY 3.0 Å, Human Amylin2 Receptor in complex with Gs and salmon calcitonin peptide
7TYH 3.3 Å, Human Amylin2 Receptor in complex with Gs and human calcitonin peptide

Browse structure collections

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