9G27: DNA-directed RNA polymerase I subunit RPA190

Yeast RNA polymerase I elongation complex stalled by an apurinic site, pre-translocation state. Determined by electron microscopy at 2.8 Å resolution. Released 18 Jun 2025.

Method: Electron microscopy
Resolution: 2.8 Å
Organism: Saccharomyces cerevisiae
Chains: 15
Atoms: 31,262
Mol. weight: 544.31 kDa
Ligands: ZN
Released: 18 Jun 2025

Explore 9G27 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9G27 contains 190 α-helices and 220 β-strands across 12 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 82 helices, 63 β-strands

Element	Residues	Length	Sheet
β-strand	11-18	8	1
α-helix	21-27	7
β-strand	31	1	2
β-strand	38	1	3
β-strand	44	1	3
α-helix	45	1
α-helix	52-54	3
β-strand	57	1	4
β-strand	60	1	4
β-strand	77-86	10	2
α-helix	91-98	8
α-helix	112-125	14
α-helix	130-135	6
α-helix	137-139	3
α-helix	175-199	25
α-helix	210-228	19
β-strand	242-245	4	5
β-strand	251-254	4	5
α-helix	255-257	3
α-helix	258-264	7
β-strand	313-314	2	5
α-helix	317-330	14
α-helix	332-338	7
α-helix	348-350	3
α-helix	352-355	4
β-strand	356-362	7	2
α-helix	363-364	2
α-helix	365-368	4
β-strand	371-373	3	6
β-strand	376-378	3	6
α-helix	381-408	28
α-helix	413-438	26
α-helix	452-454	3
α-helix	458-460	3
α-helix	466-470	5
β-strand	474-476	3	7
β-strand	479-486	8	8
β-strand	494-497	4	8
α-helix	499-504	6
β-strand	506-510	5	9
α-helix	516-525	10
β-strand	533-537	5	9
β-strand	543-545	3	9
α-helix	551-559	9
α-helix	568-570	3
β-strand	576-580	5	9
β-strand	586-590	5	8
α-helix	597-599	3
β-strand	602-607	6	8
β-strand	614-616	3	8
α-helix	620-624	5
β-strand	632-636	5	8
α-helix	641-645	5
α-helix	646-650	5
β-strand	652	1	8
α-helix	654-656	3
β-strand	658	1	10
β-strand	665	1	10
α-helix	672-680	9
β-strand	686-687	2	11
α-helix	689-699	11
α-helix	702-704	3
β-strand	719-721	3	12
β-strand	725	1	12
β-strand	726-727	2	11
α-helix	728-739	12
α-helix	745-747	3
β-strand	750-752	3	13
β-strand	768-771	4	13
β-strand	776-778	3	13
α-helix	793-823	31
β-strand	826-827	2	14
α-helix	831-833	3
β-strand	835	1	15
α-helix	837-847	11
α-helix	848-850	3
α-helix	853-861	9
α-helix	873-884	12
α-helix	886-911	26
β-strand	916	1	15
α-helix	924-931	8
α-helix	937-944	8
β-strand	948-949	2	16
α-helix	950-951	2
β-strand	952	1	17
β-strand	955	1	17
α-helix	970	1
α-helix	976-979	4
β-strand	981-982	2	16
α-helix	992-1012	21
α-helix	1017-1027	11
β-strand	1031-1032	2	18
β-strand	1038-1039	2	18
β-strand	1045-1047	3	18
α-helix	1050-1052	3
β-strand	1055	1	19
α-helix	1057-1059	3
α-helix	1061-1063	3
α-helix	1066-1071	6
α-helix	1073-1080	8
α-helix	1082-1084	3
β-strand	1090	1	20
α-helix	1093-1107	15
α-helix	1123-1126	4
β-strand	1132	1	20
α-helix	1138-1150	13
α-helix	1163-1176	14
β-strand	1178	1	19
α-helix	1179-1180	2
α-helix	1185-1198	14
α-helix	1218-1222	5
α-helix	1223-1227	5
β-strand	1237-1241	5	21
β-strand	1242	1	22
α-helix	1248-1258	11
β-strand	1261-1262	2	23
α-helix	1263-1265	3
β-strand	1267-1272	6	24
β-strand	1273-1274	2	25
β-strand	1288-1296	9	24
α-helix	1299-1306	8
α-helix	1310-1316	7
α-helix	1317-1321	5
α-helix	1322-1337	16
α-helix	1441-1453	13
β-strand	1457	1	24
β-strand	1469-1476	8	24
α-helix	1487-1495	9
β-strand	1497-1499	3	23
β-strand	1507-1508	2	21
β-strand	1518-1522	5	21
α-helix	1526-1529	4
β-strand	1536	1	22
α-helix	1538-1540	3
β-strand	1542-1543	2	21
α-helix	1546-1553	8
α-helix	1555-1572	18
α-helix	1579-1589	11
α-helix	1600-1603	4
α-helix	1609-1615	7
α-helix	1618-1627	10
β-strand	1631-1632	2	1
α-helix	1637-1643	7
α-helix	1650-1652	3
β-strand	1654-1660	7	26

Chain B: 59 helices, 80 β-strands

Element	Residues	Length	Sheet
β-strand	14	1	27
α-helix	18-26	9
α-helix	37-40	4
α-helix	43-51	9
α-helix	56-58	3
α-helix	60-68	9
β-strand	71	1	28
β-strand	96-105	10	28
α-helix	106-107	2
β-strand	108-109	2	29
β-strand	119-120	2	29
α-helix	123-129	7
β-strand	134-145	12	28
β-strand	150-162	13	28
α-helix	177-182	6
β-strand	194-196	3	30
β-strand	199-203	5	30
β-strand	205-209	5	31
α-helix	210	1
β-strand	211	1	32
β-strand	214-216	3	32
α-helix	220-224	5
β-strand	229	1	33
β-strand	234-239	6	32
β-strand	245-252	8	32
β-strand	253	1	33
β-strand	258-263	6	32
β-strand	268-272	5	32
α-helix	273-280	8
α-helix	285-292	8
α-helix	300-316	17
α-helix	323-333	11
α-helix	346-356	11
α-helix	360-362	3
α-helix	367-385	19
α-helix	397-399	3
β-strand	400-403	4	31
α-helix	407-434	28
α-helix	443-450	8
α-helix	457-467	11
β-strand	485-487	3	30
α-helix	493-499	7
β-strand	502-504	3	31
α-helix	522-524	3
β-strand	528	1	34
β-strand	542-544	3	31
β-strand	545	1	34
β-strand	550-551	2	35
α-helix	561-568	8
α-helix	570	1
β-strand	571-572	2	36
β-strand	584-588	5	36
β-strand	591-596	6	36
α-helix	598-613	16
β-strand	625-629	5	36
α-helix	637-638	2
β-strand	639-643	5	36
β-strand	649-650	2	35
β-strand	651-655	5	37
α-helix	657-659	3
β-strand	660-664	5	37
α-helix	666-669	4
β-strand	674-675	2	37
α-helix	678-680	3
β-strand	688-689	2	37
α-helix	693-696	4
α-helix	699-702	4
α-helix	707-709	3
α-helix	712-721	10
β-strand	725	1	38
α-helix	733-735	3
β-strand	740-744	5	39
β-strand	748	1	40
β-strand	752-753	2	41
α-helix	756-759	4
α-helix	762-764	3
β-strand	769-772	4	42
β-strand	773-775	3	43
β-strand	776-777	2	14
β-strand	787-790	4	43
α-helix	791-794	4
β-strand	801-810	10	39
α-helix	819-821	3
β-strand	823-825	3	44
α-helix	834-837	4
β-strand	840	1	45
β-strand	846	1	45
α-helix	847	1
β-strand	851-852	2	39
β-strand	860-863	4	44
β-strand	868-871	4	44
β-strand	880-888	9	39
β-strand	900-909	10	39
β-strand	916-919	4	42
β-strand	925-926	2	42
β-strand	927-931	5	43
β-strand	938-939	2	46
α-helix	943-945	3
β-strand	947-949	3	43
α-helix	951-953	3
α-helix	954-957	4
α-helix	960-975	16
β-strand	978	1	27
β-strand	979-980	2	41
α-helix	992-1003	12
β-strand	1010-1011	2	43
β-strand	1013-1014	2	46
β-strand	1021	1	46
α-helix	1022	1
β-strand	1026-1027	2	43
β-strand	1030-1035	6	42
β-strand	1036	1	38
α-helix	1040-1042	3
β-strand	1045-1047	3	8
α-helix	1051	1
β-strand	1052	1	47
α-helix	1053	1
α-helix	1058	1
β-strand	1059	1	47
α-helix	1060	1
α-helix	1067-1068	2
β-strand	1069-1071	3	7
α-helix	1073-1081	9
α-helix	1085-1089	5
α-helix	1090-1094	5
β-strand	1098-1100	3	48
β-strand	1103-1104	2	49
β-strand	1113-1115	3	50
α-helix	1116	1
β-strand	1117	1	51
α-helix	1118	1
β-strand	1126-1134	9	50
β-strand	1159	1	50
β-strand	1160	1	51
β-strand	1167-1170	4	50
β-strand	1173-1174	2	49
β-strand	1177-1179	3	48
α-helix	1180-1191	12
β-strand	1194-1200	7	1

Chain C: 15 helices, 21 β-strands

Element	Residues	Length	Sheet
α-helix	33-39	7
β-strand	41-47	7	52
β-strand	51-57	7	52
α-helix	61-73	13
β-strand	77-85	9	53
β-strand	87-88	2	54
α-helix	94-102	9
β-strand	106	1	55
α-helix	110-112	3
α-helix	122-125	4
β-strand	132-135	4	53
β-strand	136-139	4	54
β-strand	141-142	2	56
α-helix	146-147	2
α-helix	153-156	4
β-strand	157-158	2	56
β-strand	161-163	3	57
α-helix	164-166	3
β-strand	168-170	3	53
α-helix	175-178	4
β-strand	186	1	55
β-strand	192-195	4	57
β-strand	201-204	4	54
β-strand	206-211	6	53
α-helix	217-219	3
β-strand	222-226	5	52
β-strand	229-235	7	52
α-helix	242-249	8
β-strand	256-257	2	58
β-strand	266-267	2	58
α-helix	270-272	3
α-helix	279-281	3
α-helix	283-286	4
β-strand	289-299	11	52
β-strand	301-304	4	52
α-helix	310-330	21

Chain D: 0 helices, 1 β-strand

Element	Residues	Length	Sheet
β-strand	23-24	2	26

Chain E: 9 helices, 14 β-strands

Element	Residues	Length	Sheet
α-helix	5-26	22
β-strand	28	1	59
α-helix	32-35	4
α-helix	39-46	8
β-strand	47	1	60
β-strand	53	1	60
β-strand	61-62	2	61
β-strand	64	1	59
α-helix	66-71	6
β-strand	78-82	5	61
β-strand	87-88	2	62
α-helix	90-103	14
β-strand	107-112	6	61
β-strand	115-116	2	62
α-helix	118-121	4
α-helix	124-126	3
β-strand	131-136	6	61
β-strand	152-155	4	63
α-helix	158-168	11
β-strand	177-178	2	63
α-helix	183-188	6
β-strand	195-202	8	63
β-strand	206-214	9	63

Chain F: 6 helices, 5 β-strands

Element	Residues	Length	Sheet
α-helix	55-72	18
β-strand	73	1	26
β-strand	84-85	2	64
α-helix	86	1
α-helix	87-102	16
α-helix	110-111	2
α-helix	117-127	11
β-strand	132-137	6	26
α-helix	138	1
β-strand	143-147	5	26
β-strand	152-153	2	64

Chain G: 5 helices, 6 β-strands

Element	Residues	Length	Sheet
β-strand	39-49	11	26
α-helix	51-53	3
α-helix	57-61	5
α-helix	62-66	5
α-helix	67-69	3
β-strand	73	1	65
β-strand	80	1	65
β-strand	81-89	9	26
β-strand	103-107	5	26
α-helix	108	1
β-strand	114-124	11	26

Chain H: 2 helices, 9 β-strands

Element	Residues	Length	Sheet
β-strand	4-16	13	12
β-strand	23-30	8	12
β-strand	37-43	7	12
β-strand	55-60	6	12
α-helix	89-92	4
β-strand	95-98	4	12
β-strand	100-108	9	12
β-strand	111-118	8	12
β-strand	121-127	7	12
α-helix	129-132	4
β-strand	141-144	4	12

4 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
DNA-directed RNA polymerase I subunit RPA190	A	protein	1664	Saccharomyces cerevisiae	P10964 (AlphaFold model)
DNA-directed RNA polymerase I subunit RPA135	B	protein	1203	Saccharomyces cerevisiae	P22138 (AlphaFold model)
DNA-directed RNA polymerases I and III subunit RPAC1	C	protein	335	Saccharomyces cerevisiae	P07703 (AlphaFold model)
DNA-directed RNA polymerase I subunit RPA14	D	protein	137	Saccharomyces cerevisiae	P50106 (AlphaFold model)
DNA-directed RNA polymerases I, II, and III subunit RPABC1	E	protein	215	Saccharomyces cerevisiae	P20434
DNA-directed RNA polymerases I, II, and III subunit RPABC2	F	protein	155	Saccharomyces cerevisiae	P20435
DNA-directed RNA polymerase I subunit RPA43	G	protein	326	Saccharomyces cerevisiae	P46669
DNA-directed RNA polymerases I, II, and III subunit RPABC3	H	protein	146	Saccharomyces cerevisiae	P20436
DNA-directed RNA polymerase I subunit RPA12	I	protein	125	Saccharomyces cerevisiae	P32529
DNA-directed RNA polymerases I, II, and III subunit RPABC5	J	protein	70	Saccharomyces cerevisiae	P22139
DNA-directed RNA polymerases I and III subunit RPAC2	K	protein	142	Saccharomyces cerevisiae	P28000
DNA-directed RNA polymerases I, II, and III subunit RPABC4	L	protein	70	Saccharomyces cerevisiae	P40422

3 more molecules are not listed.

Sequence of entity 1 (A), FASTA

>9G27_1 DNA-directed RNA polymerase I subunit RPA190 (chains A)
MDISKPVGSEITSVDFGILTAKEIRNLSAKQITNPTVLDNLGHPVSGGLYDLALGAFLRN
LCSTCGLDEKFCPGHQGHIELPVPCYNPLFFNQLYIYLRASCLFCHHFRLKSVEVHRYAC
KLRLLQYGLIDESYKLDEITLGSLNSSMYTDDEAIEDNEDEMDGEGSKQSKDISSTLLNE
LKSKRSEYVDMAIAKALSDGRTTERGSFTATVNDERKKLVHEFHKKLLSRGKCDNCGMFS
PKFRKDGFTKIFETALNEKQITNNRVKGFIRQDMIKKQKQAKKLDGSNEASANDEESFDV
GRNPTTRPKTGSTYILSTEVKNILDTVFRKEQCVLQYVFHSRPNLSRKLVKADSFFMDVL
VVPPTRFRLPSKLGEEVHENSQNQLLSKVLTTSLLIRDLNDDLSKLQKDKVSLEDRRVIF
SRLMNAFVTIQNDVNAFIDSTKAQGRTSGKVPIPGVKQALEKKEGLFRKHMMGKRVNYAA
RSVISPDPNIETNEIGVPPVFAVKLTYPEPVTAYNIAELRQAVINGPDKWPGATQIQNED
GSLVSLIGMSVEQRKALANQLLTPSSNVSTHTLNKKVYRHIKNRDVVLMNRQPTLHKASM
MGHKVRVLPNEKTLRLHYANTGAYNADFDGDEMNMHFPQNENARAEALNLANTDSQYLTP
TSGSPVRGLIQDHISAGVWLTSKDSFFTREQYQQYIYGCIRPEDGHTTRSKIVTLPPTIF
KPYPLWTGKQIITTVLLNVTPPDMPGINLISKNKIKNEYWGKGSLENEVLFKDGALLCGI
LDKSQYGASKYGIVHSLHEVYGPEVAAKVLSVLGRLFTNYITATAFTCGMDDLRLTAEGN
KWRTDILKTSVDTGREAAAEVTNLDKDTPADDPELLKRLQEILRDNNKSGILDAVTSSKV
NAITSQVVSKCVPDGTMKKFPCNSMQAMALSGAKGSNVNVSQIMCLLGQQALEGRRVPVM
VSGKTLPSFKPYETDAMAGGYVKGRFYSGIKPQEYYFHCMAGREGLIDTAVKTSRSGYLQ
RCLTKQLEGVHVSYDNSIRDADGTLVQFMYGGDAIDITKESHMTQFEFCLDNYYALLKKY
NPSALIEHLDVESALKYSKKTLKYRKKHSKEPHYKQSVKYDPVLAKYNPAKYLGSVSENF
QDKLESFLDKNSKLFKSSDGVNEKKFRALMQLKYMRSLINPGEAVGIIASQSVGEPSTQM
TLNTFHFAGHGAANVTLGIPRLREIVMTASAAIKTPQMTLPIWNDVSDEQADTFCKSISK
VLLSEVIDKVIVTETTGTSNTAGGNAARSYVIHMRFFDNNEYSEEYDVSKEELQNVISNQ
FIHLLEAAIVKEIKKQKRTTGPDIGVAVPRLQTDVANSSSNSKRLEEDNDEEQSHKKTKQ
AVSYDEPDEDEIETMREAEKSSDEEGIDSDKESDSDSEDEDVDMNEQINKSIVEANNNMN
KVQRDRQSAIISHHRFITKYNFDDESGKWCEFKLELAADTEKLLMVNIVEEICRKSIIRQ
IPHIDRCVHPEPENGKRVLVTEGVNFQAMWDQEAFIDVDGITSNDVAAVLKTYGVEAARN
TIVNEINNVFSRYAISVSFRHLDLIADMMTRQGTYLAFNRQGMETSTSSFMKMSYETTCQ
FLTKAVLDNEREQLDSPSARIVVGKLNNVGTGSFDVLAKVPNAA

Sequence of entity 2 (B), FASTA

>9G27_2 DNA-directed RNA polymerase I subunit RPA135 (chains B)
MSKVIKPPGQARTADFRTLERESRFINPPKDKSAFPLLQEAVQPHIGSFNALTEGPDGGL
LNLGVKDIGEKVIFDGKPLNSEDEISNSGYLGNKLSVSVEQVSIAKPMSNDGVSSAVERK
VYPSESRQRLTSYRGKLLLKLKWSVNNGEENLFEVRDCGGLPVMLQSNRCHLNKMSPYEL
VQHKEESDEIGGYFIVNGIEKLIRMLIVQRRNHPMAIIRPSFANRGASYSHYGIQIRSVR
PDQTSQTNVLHYLNDGQVTFRFSWRKNEYLVPVVMILKALCHTSDREIFDGIIGNDVKDS
FLTDRLELLLRGFKKRYPHLQNRTQVLQYLGDKFRVVFQASPDQSDLEVGQEVLDRIVLV
HLGKDGSQDKFRMLLFMIRKLYSLVAGECSPDNPDATQHQEVLLGGFLYGMILKEKIDEY
LQNIIAQVRMDINRGMAINFKDKRYMSRVLMRVNENIGSKMQYFLSTGNLVSQSGLDLQQ
VSGYTVVAEKINFYRFISHFRMVHRGSFFAQLKTTTVRKLLPESWGFLCPVHTPDGSPCG
LLNHFAHKCRISTQQSDVSRIPSILYSLGVAPASHTFAAGPSLCCVQIDGKIIGWVSHEQ
GKIIADTLRYWKVEGKTPGLPIDLEIGYVPPSTRGQYPGLYLFGGHSRMLRPVRYLPLDK
EDIVGPFEQVYMNIAVTPQEIQNNVHTHVEFTPTNILSILANLTPFSDFNQSPRNMYQCQ
MGKQTMGTPGVALCHRSDNKLYRLQTGQTPIVKANLYDDYGMDNFPNGFNAVVAVISYTG
YDMDDAMIINKSADERGFGYGTMYKTEKVDLALNRNRGDPITQHFGFGNDEWPKEWLEKL
DEDGLPYIGTYVEEGDPICAYFDDTLNKTKIKTYHSSEPAYIEEVNLIGDESNKFQELQT
VSIKYRIRRTPQIGDKFSSRHGQKGVCSRKWPTIDMPFSETGIQPDIIINPHAFPSRMTI
GMFVESLAGKAGALHGIAQDSTPWIFNEDDTPADYFGEQLAKAGYNYHGNEPMYSGATGE
ELRADIYVGVVYYQRLRHMVNDKFQVRSTGPVNSLTMQPVKGRKRHGGIRVGEMERDALI
GHGTSFLLQDRLLNSSDYTQASVCRECGSILTTQQSVPRIGSISTVCCRRCSMRFEDAKK
LLTKSEDGEKIFIDDSQIWEDGQGNKFVGGNETTTVAIPFVLKYLDSELSAMGIRLRYNV
EPK

Sequence of entity 3 (C), FASTA

>9G27_3 DNA-directed RNA polymerases I and III subunit RPAC1 (chains C)
MSNIVGIEYNRVTNTTSTDFPGFSKDAENEWNVEKFKKDFEVNISSLDAREANFDLINID
TSIANAFRRIMISEVPSVAAEYVYFFNNTSVIQDEVLAHRIGLVPLKVDPDMLTWVDSNL
PDDEKFTDENTIVLSLNVKCTRNPDAPKGSTDPKELYNNAHVYARDLKFEPQGRQSTTFA
DCPVVPADPDILLAKLRPGQEISLKAHCILGIGGDHAKFSPVSTASYRLLPQINILQPIK
GESARRFQKCFPPGVIGIDEGSDEAYVKDARKDTVSREVLRYEEFADKVKLGRVRNHFIF
NVESAGAMTPEEIFFKSVRILKNKAEYLKNCPITQ

Sequence of entity 4 (D), FASTA

>9G27_4 DNA-directed RNA polymerase I subunit RPA14 (chains D)
MMKGSRRTGNNTATTLNTPVVIHATQLPQHVSTDEVLQFLESFIDEKENIIDSTTMNTIS
GNAADADAAAVANTSLNIDTNLSSSISQLKRIQRDFKGLPPAQDFSAAPIQVSTTEKKET
SIGVSATGGKKTTFADE

Sequence of entity 5 (E), FASTA

>9G27_5 DNA-directed RNA polymerases I, II, and III subunit RPABC1 (chains E)
MDQENERNISRLWRAFRTVKEMVKDRGYFITQEEVELPLEDFKAKYCDSMGRPQRKMMSF
QANPTEESISKFPDMGSLWVEFCDEPSVGVKTMKTFVIHIQEKNFQTGIFVYQNNITPSA
MKLVPSIPPATIETFNEAALVVNITHHELVPKHIRLSSDEKRELLKRYRLKESQLPRIQR
ADPVALYLGLKRGEVVKIIRKSETSGRYASYRICM

Sequence of entity 6 (F), FASTA

>9G27_6 DNA-directed RNA polymerases I, II, and III subunit RPABC2 (chains F)
MSDYEEAFNDGNENFEDFDVEHFSDEETYEEKPQFKDGETTDANGKTIVTGGNGPEDFQQ
HEQIRRKTLKEKAIPKDQRATTPYMTKYERARILGTRALQISMNAPVFVDLEGETDPLRI
AMKELAEKKIPLVIRRYLPDGSFEDWSVEELIVDL

Sequence of entity 7 (G), FASTA

>9G27_7 DNA-directed RNA polymerase I subunit RPA43 (chains G)
MSQVKRANENRETARFIKKHKKQVTNPIDEKNGTSNCIVRVPIALYVSLAPMYLENPLQG
VMKQHLNPLVMKYNNKVGGVVLGYEGLKILDADPLSKEDTSEKLIKITPDTPFGFTWCHV
NLYVWQPQVGDVLEGYIFIQSASHIGLLIHDAFNASIKKNNIPVDWTFVHNDVEEDADVI
NTDENNGNNNNEDNKDSNGGSNSLGKFSFGNRSLGHWVDSNGEPIDGKLRFTVRNVHTTG
RVVSVDGTLISDADEEGNGYNSSRSQAESLPIVSNKKIVFDDEVSIENKESHKELDLPEV
KEDNGSEIVYEENTSESNDGESSDSD

Sequence of entity 8 (H), FASTA

>9G27_8 DNA-directed RNA polymerases I, II, and III subunit RPABC3 (chains H)
MSNTLFDDIFQVSEVDPGRYNKVCRIEAASTTQDQCKLTLDINVELFPVAAQDSLTVTIA
SSLNLEDTPANDSSATRSWRPPQAGDRSLADDYDYVMYGTAYKFEEVSKDLIAVYYSFGG
LLMRLEGNYRNLNNLKQENAYLLIRR

Sequence of entity 9 (I), FASTA

>9G27_9 DNA-directed RNA polymerase I subunit RPA12 (chains I)
MSVVGSLIFCLDCGDLLENPNAVLGSNVECSQCKAIYPKSQFSNLKVVTTTADDAFPSSL
RAKKSVVKTSLKKNELKDGATIKEKCPQCGNEEMNYHTLQLRSADEGATVFYTCTSCGYK
FRTNN

Sequence of entity 10 (J), FASTA

>9G27_10 DNA-directed RNA polymerases I, II, and III subunit RPABC5 (chains J)
MIVPVRCFSCGKVVGDKWESYLNLLQEDELDEGTALSRLGLKRYCCRRMILTHVDLIEKF
LRYNPLEKRD

Sequence of entity 11 (K), FASTA

>9G27_11 DNA-directed RNA polymerases I and III subunit RPAC2 (chains K)
MTEDIEQKKTATEVTPQEPKHIQEEEEQDVDMTGDEEQEEEPDREKIKLLTQATSEDGTS
ASFQIVEEDHTLGNALRYVIMKNPDVEFCGYSIPHPSENLLNIRIQTYGETTAVDALQKG
LKDLMDLCDVVESKFTEKIKSM

Sequence of entity 12 (L), FASTA

>9G27_12 DNA-directed RNA polymerases I, II, and III subunit RPABC4 (chains L)
MSREGFQIPTNLDAAAAGTSQARTATLKYICAECSSKLSLSRTDAVRCKDCGHRILLKAR
TKRLVQFEAR

Ligands and cofactors

ID	Name	Formula	Copies
ZN	Zinc ion	Zn	6

Primary citation

Cryo-EM uncovers a sequential mechanism for RNA polymerase I pausing and stalling at abasic DNA lesions. Santos-Aledo, A., Plaza-Pegueroles, A., Sanz-Murillo, M. et al. Nat Commun (2025) 16:5254-5254. DOI 10.1038/s41467-025-60536-4 · PubMed

Other PDB entries of the same protein (UniProt P10964 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6RUI 2.7 Å, RNA Polymerase I Pre-initiation complex DNA opening intermediate 2
9G1V 2.7 Å, Yeast RNA polymerase I elongation complex stalled by an apurinic site
4C2M 2.8 Å, Structure of RNA polymerase I at 2.8 A resolution
6RQL 2.9 Å, RNA Polymerase I Closed Conformation 2 (CC2)
4C3I 3.0 Å, Structure of 14-subunit RNA polymerase I at 3.0 A resolution, crystal form C2-100
6RWE 3.0 Å, RNA Polymerase I Open Complex conformation 2
6RRD 3.1 Å, RNA Polymerase I Pre-initiation complex DNA opening intermediate 1
6HLQ 3.18 Å, Yeast RNA polymerase I* elongation complex bound to nucleotide analog GMPCPP
6HLR 3.18 Å, Yeast RNA polymerase I elongation complex bound to nucleotide analog GMPCPP (core focused)
9G2B 3.2 Å, Yeast RNA polymerase I elongation complex stalled by an apurinic site, 12-subunit
6HLS 3.21 Å, Yeast apo RNA polymerase I*
4C3H 3.27 Å, Structure of 14-subunit RNA polymerase I at 3.27 A resolution, crystal form C2-93

Browse structure collections

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