9GU3: Acetylcholine receptor subunit alpha

Human adult muscle nAChR in desensitised state in nanodisc with 1 mM acetylcholine. Determined by electron microscopy at 2.64 Å resolution. Released 14 May 2025.

Method: Electron microscopy
Resolution: 2.64 Å
Organisms: Homo sapiens, Aequorea victoria, Rattus norvegicus
Chains: 9
Atoms: 19,563
Mol. weight: 392.01 kDa
Ligands: NAG, ACH
Released: 14 May 2025

Explore 9GU3 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9GU3 contains 90 α-helices and 170 β-strands across 9 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 11 helices, 14 β-strands

Element	Residues	Length	Sheet
α-helix	2-11	10
β-strand	29-44	16	1
β-strand	49-61	13	1
α-helix	63-65	3
α-helix	69-72	4
β-strand	77-81	5	1
β-strand	90-92	3	2
β-strand	95	1	1
β-strand	107-111	5	1
β-strand	115-118	4	1
β-strand	121-127	7	1
β-strand	139-148	10	2
β-strand	156-160	5	1
β-strand	166	1	1
β-strand	171	1	1
β-strand	176-188	13	2
β-strand	198-209	12	2
α-helix	212-214	3
α-helix	215-219	5
α-helix	220-231	12
α-helix	232-234	3
α-helix	237-239	3
α-helix	242-263	22
α-helix	273-296	24
α-helix	403-425	23

Chain B: 16 helices, 17 β-strands

Element	Residues	Length	Sheet
α-helix	2-12	11
α-helix	27-28	2
β-strand	29-44	16	3
β-strand	49-61	13	3
α-helix	63-65	3
α-helix	69-72	4
β-strand	77-80	4	3
α-helix	82-84	3
β-strand	90-92	3	4
β-strand	95	1	3
α-helix	106-107	2
β-strand	108-111	4	3
β-strand	115-118	4	3
β-strand	121-127	7	3
β-strand	139-148	10	4
β-strand	156-160	5	3
α-helix	161-163	3
β-strand	171	1	5
β-strand	173	1	3
β-strand	180	1	3
β-strand	185-189	5	4
β-strand	191	1	5
β-strand	192-195	4	4
β-strand	211-220	10	4
α-helix	223-225	3
α-helix	226-230	5
α-helix	231-242	12
α-helix	243-245	3
α-helix	248-250	3
α-helix	253-274	22
α-helix	284-308	25
α-helix	438-469	32
α-helix	471-473	3

Chains C and G: 5 helices, 23 β-strands

Element	Residues	Length	Sheet
β-strand	4-7	4	16
β-strand	10-13	4	17
β-strand	19-25	7	16
β-strand	33-38	6	17
β-strand	45-49	5	17
β-strand	53-54	2	17
α-helix	55	1
β-strand	62-67	6	16
β-strand	70-75	6	16
α-helix	80-82	3
β-strand	85-90	6	17
β-strand	96-97	2	17
β-strand	101-105	5	17
β-strand	110	1	18
β-strand	113-117	5	19
α-helix	118-120	3
α-helix	121-125	5
β-strand	128	1	20
β-strand	131-138	8	19
β-strand	139	1	18
β-strand	143-149	7	21
β-strand	152-154	3	21
β-strand	158-162	5	19
β-strand	172-178	7	19
β-strand	181	1	20
α-helix	182-186	5
β-strand	190-197	8	21
β-strand	204-209	6	21

Chain D: 16 helices, 19 β-strands

Element	Residues	Length	Sheet
α-helix	3-8	6
α-helix	9-14	6
α-helix	29-30	2
β-strand	31-46	16	6
β-strand	51-63	13	6
α-helix	65-67	3
α-helix	71-74	4
β-strand	79-82	4	6
β-strand	92-94	3	7
β-strand	97	1	6
α-helix	108-109	2
β-strand	110-113	4	6
β-strand	117-120	4	6
β-strand	123-129	7	6
β-strand	141-150	10	7
β-strand	158-162	5	6
α-helix	163	1
β-strand	164-167	4	8
β-strand	170-173	4	8
β-strand	176	1	9
β-strand	178	1	6
β-strand	185	1	6
β-strand	190-194	5	7
β-strand	196	1	9
β-strand	197-201	5	7
β-strand	213-223	11	7
α-helix	226-228	3
α-helix	229-233	5
α-helix	234-242	9
α-helix	243-247	5
α-helix	251-253	3
α-helix	256-277	22
α-helix	287-311	25
α-helix	446-472	27
α-helix	490-492	3

Chain E: 15 helices, 18 β-strands

Element	Residues	Length	Sheet
α-helix	3-12	10
α-helix	27-28	2
β-strand	29-44	16	10
β-strand	49-61	13	10
α-helix	63-65	3
α-helix	69-71	3
β-strand	77-80	4	10
α-helix	82-84	3
β-strand	90-92	3	11
β-strand	95	1	10
α-helix	106-107	2
β-strand	108-111	4	10
β-strand	115-118	4	10
β-strand	121-127	7	10
β-strand	139-148	10	11
β-strand	156-160	5	10
β-strand	162	1	12
β-strand	168	1	12
β-strand	171	1	13
β-strand	173	1	10
β-strand	185-189	5	11
β-strand	191	1	13
β-strand	192-197	6	11
β-strand	207-218	12	11
α-helix	221-223	3
α-helix	224-228	5
α-helix	229-238	10
α-helix	239-243	5
α-helix	252-273	22
α-helix	283-306	24
α-helix	432-459	28
α-helix	461-464	4
α-helix	468-469	2

Chain F: 4 helices, 21 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	22
β-strand	11-12	2	23
β-strand	18-25	8	22
β-strand	33-40	8	17
β-strand	46-51	6	17
β-strand	57-59	3	17
β-strand	67-72	6	22
β-strand	77-82	6	22
α-helix	87-89	3
β-strand	91-101	11	17
β-strand	104-110	7	17
β-strand	114-116	3	17
β-strand	117-118	2	23
α-helix	121-123	3
β-strand	124	1	24
α-helix	125-126	2
β-strand	127-131	5	25
β-strand	143-150	8	25
β-strand	153	1	24
β-strand	158-161	4	26
α-helix	163-166	4
β-strand	170-178	9	25
β-strand	181-190	10	25
β-strand	201-206	6	26
β-strand	211-216	6	26

Chain H: 4 helices, 21 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	33
β-strand	11-12	2	34
β-strand	18-25	8	33
β-strand	33-40	8	28
β-strand	46-51	6	28
β-strand	57-59	3	28
β-strand	67-72	6	33
β-strand	77-82	6	33
α-helix	87-89	3
β-strand	91-101	11	28
β-strand	104-110	7	28
β-strand	114-116	3	28
β-strand	117-118	2	34
α-helix	121-123	3
β-strand	124	1	35
α-helix	125-126	2
β-strand	127-131	5	36
β-strand	143-152	10	36
β-strand	153	1	35
β-strand	158-161	4	37
α-helix	163-166	4
β-strand	170-178	9	36
β-strand	181-190	10	36
β-strand	201-206	6	37
β-strand	211-216	6	37

Chain L: 14 helices, 14 β-strands

Element	Residues	Length	Sheet
α-helix	3-11	9
β-strand	29-44	16	14
β-strand	49-61	13	14
α-helix	63-65	3
α-helix	69-72	4
β-strand	77-81	5	14
α-helix	82-84	3
β-strand	90-92	3	15
β-strand	95	1	14
α-helix	106	1
β-strand	107-111	5	14
β-strand	115-118	4	14
β-strand	121-127	7	14
β-strand	139-148	10	15
β-strand	156-160	5	14
β-strand	166	1	14
α-helix	170	1
β-strand	171	1	14
α-helix	172-173	2
β-strand	176-188	13	15
β-strand	198-209	12	15
α-helix	212-214	3
α-helix	215-219	5
α-helix	220-234	15
α-helix	237-239	3
α-helix	241-263	23
α-helix	273-297	25
α-helix	407-429	23

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Acetylcholine receptor subunit alpha	A, L	protein	437	Homo sapiens	P02708 (AlphaFold model)
Acetylcholine receptor subunit beta	B	protein	478	Homo sapiens	P11230 (AlphaFold model)
Acetylcholine receptor subunit delta	D	protein	496	Homo sapiens	Q07001 (AlphaFold model)
Acetylcholine receptor subunit epsilon,Green fluorescent protein	E	protein	721	Homo sapiens, Aequorea victoria	Q04844 (AlphaFold model)
Fab35 light chain	C, G	protein	213	Rattus norvegicus
Fab35 heavy chain	F, H	protein	219	Rattus norvegicus

Sequence of entity 1 (A, L), FASTA

>9GU3_1 Acetylcholine receptor subunit alpha (chains A, L)
SEHETRLVAKLFKDYSSVVRPVEDHRQVVEVTVGLQLIQLINVDEVNQIVTTNVRLKQQW
VDYNLKWNPDDYGGVKKIHIPSEKIWRPDLVLYNNADGDFAIVKFTKVLLQYTGHITWTP
PAIFKSYCEIIVTHFPFDEQNCSMKLGTWTYDGSVVAINPESDQPDLSNFMESGEWVIKE
SRGWKHSVTYSCCPDTPYLDITYHFVMQRLPLYFIVNVIIPCLLFSFLTGLVFYLPTDSG
EKMTLSISVLLSLTVFLLVIVELIPSTSSAVPLIGKYMLFTMVFVIASIIITVIVINTHH
RSPSTHVMPNWVRKVFIDTIPNIMFFSTMKRPSREKQDKKIFTEDIDISDISGKPGPPPM
GFHSPLIKHPEVKSAIEGIKYIAETMKSDQESNNAAAEWKYVAMVMDHILLGVFMLVCII
GTLAVFAGRLIELNQQG

Sequence of entity 2 (B), FASTA

>9GU3_2 Acetylcholine receptor subunit beta (chains B)
SEAEGRLREKLFSGYDSSVRPAREVGDRVRVSVGLILAQLISLNEKDEEMSTKVYLDLEW
TDYRLSWDPAEHDGIDSLRITAESVWLPDVVLLNNNDGNFDVALDISVVVSSDGSVRWQP
PGIYRSSCSIQVTYFPFDWQNCTMVFSSYSYDSSEVSLQTGLGPDGQGHQEIHIHEGTFI
ENGQWEIIHKPSRLIQPPGDPRGGREGQRQEVIFYLIIRRKPLFYLVNVIAPCILITLLA
IFVFYLPPDAGEKMGLSIFALLTLTVFLLLLADKVPETSLSVPIIIKYLMFTMVLVTFSV
ILSVVVLNLHHRSPHTHQMPLWVRQIFIHKLPLYLRLKRPKPERDLMPEPPHCSSPGSGW
GRGTDEYFIRKPPSDFLFPKPNRFQPELSAPDLRRFIDGPNRAVALLPELREVVSSISYI
ARQLQEQEDHDALKEDWQFVAMVVDRLFLWTFIIFTSVGTLVIFLDATYHLPPPDPFP

Sequence of entity 3 (D), FASTA

>9GU3_3 Acetylcholine receptor subunit delta (chains D)
LNEEERLIRHLFQEKGYNKELRPVAHKEESVDVALALTLSNLISLKEVEETLTTNVWIEH
GWTDNRLKWNAEEFGNISVLRLPPDMVWLPEIVLENNNDGSFQISYSCNVLVYHYGFVYW
LPPAIFRSSCPISVTYFPFDWQNCSLKFSSLKYTAKEITLSLKQDAKENRTYPVEWIIID
PEGFTENGEWEIVHRPARVNVDPRAPLDSPSRQDITFYLIIRRKPLFYIINILVPCVLIS
FMVNLVFYLPADSGEKTSVAISVLLAQSVFLLLISKRLPATSMAIPLIGKFLLFGMVLVT
MVVVICVIVLNIHFRTPSTHVLSEGVKKLFLETLPELLHMSRPAEDGPSPGALVRRSSSL
GYISKAEEYFLLKSRSDLMFEKQSERHGLARRLTTARRPPASSEQAQQELFNELKPAVDG
ANFIVNHMRDQNNYNEEKDSWNRVARTVDRLCLFVVTPVMVVGTAWIFLQGVYNQPPPQP
FPGDPYSYNVQDKRFI

Sequence of entity 4 (E), FASTA

>9GU3_4 Acetylcholine receptor subunit epsilon,Green fluorescent protein (chains E)
KNEELRLYHHLFNNYDPGSRPVREPEDTVTISLKVTLTNLISLNEKEETLTTSVWIGIDW
QDYRLNYSKDDFGGIETLRVPSELVWLPEIVLENNIDGQFGVAYDANVLVYEGGSVTWLP
PAIYRSVCAVEVTYFPFDWQNCSLIFRSQTYNAEEVEFTFAVDNDGKTINKIDIDTEAYT
ENGEWAIDFCPGVIRRHHGGATDGPGETDVIYSLIIRRKPLFYVINIIVPCVLISGLVLL
AYFLPAQAGGQKCTVSINVLLAQTVFLFLIAQKIPETSLSVPLLGRFLIFVMVVATLIVM
NCVIVLNVSQRTPTTHAMSPRLRHVLLELLPRLLGSPPPPEAPRAPPVATMVSKGEELFT
GVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQ
CFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGID
FKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIG
DGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKAPRAASPPRRA
SSVGLLLRAEELILKKPRSELVFEGQRHRQGTWTAAFCQSLGAAAPEVRCCVDAVNFVAE
STRDQEATGEEVSDWVRMGNALDNICFWAALVLFSVGSSLIFLGAYFNRVPDLPYAPCIQ
P

Sequence of entity 5 (C, G), FASTA

>9GU3_5 Fab35 light chain (chains C, G)
DIVITQSPSLLSASVGDRVTLTCKGSQNIDNYLAWYQQKLGEAPKLLIYKTNSLQTGIPS
RFSGSGSGTDYTLTISSLHSEDLATYYCYQYINGYTFGTGTKLELKRADAAPTVSIFPPS
TEQLATGGASVVCLMNNFYPRDISVKWKIDGTERRDGVLDSVTDQDSKDSTYSMSSTLSL
TKADYESHNLYTCEVVHKTSSSPVVKSFNRNEC

Sequence of entity 6 (F, H), FASTA

>9GU3_6 Fab35 heavy chain (chains F, H)
EVQLQESGPGLVQPSETLSLTCTVSGFSLTSYSVSWLRQPSGKGPEWMGRMWDDGGTVYN
SGLKSRLSISRDTSKNQVFLKMNSLQTDDTGTYYCTRDERIRAINWFAYWGQGTLVTVSS
AETTAPSVYPLAPGTALKSNSMVTLGCLVKGYFPEPVTVTWNSGALSSGVHTFPAVLQSG
LYTLTSSVTVPSSTWPSQTVTCNVAHPGQQHQRWTRKLC

Ligands and cofactors

ID	Name	Formula	Copies
NAG	2-acetamido-2-deoxy-beta-D-glucopyranose	C8 H15 N O6	1
ACH	Acetylcholine	C7 H16 N O2	2

Primary citation

Structures of the human adult muscle-type nicotinic receptor in resting and desensitized states. Li, A., Pike, A.C.W., Webster, R. et al. Cell Rep (2025) 44:115581-115581. DOI 10.1016/j.celrep.2025.115581 · PubMed

Other PDB entries of the same protein (UniProt P02708 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9DMS 1.92 Å, Human muscle nAChR with fab6-bound
9DMG 2.05 Å, Human muscle nAChR apo state
9DMH 2.06 Å, Human muscle nAChR ACh-bound state
9DMQ 2.06 Å, Human muscle nAChR with fab3-bound
9DMV 2.13 Å, Human muscle nAChR with fab9-bound
9DMT 2.18 Å, Human muscle nAChR with fab7-bound
9DMJ 2.19 Å, Human muscle nAChR with two fab1b-bound
9YER 2.19 Å, Human muscle nAChR SCCMS eT264P ACh bound
9YE8 2.22 Å, Human muscle nAChR SCCMS eL269F ACh bound
4ZJS 2.23 Å, Crystal structure of a chimeric acetylcholine binding protein from Aplysia Californica…
9DML 2.24 Å, Human muscle nAChR with fab2-bound
9YEI 2.36 Å, Human muscle nAChR SCCMS eL269F ACh, Reboxetine bound (lower 1)

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