9J0R: PcStar in the green fluorescent state

Name: PDB 9J0R: Structure of pcStar in the green fluorescent state
Published: 2025-04-16
License: https://creativecommons.org/publicdomain/zero/1.0/
Keywords: fluorescent protein

Structure of pcStar in the green fluorescent state. Determined by X-ray diffraction at 1.66 Å resolution. Released 16 Apr 2025.

Method: X-ray diffraction
Resolution: 1.66 Å
Organism: Lobophyllia hemprichii
Chains: 2
Atoms: 3,957
Mol. weight: 52.49 kDa
Ligands: DTT
Released: 16 Apr 2025

Explore 9J0R in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9J0R contains 11 α-helices and 26 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 5 helices, 13 β-strands

Element	Residues	Length	Sheet
β-strand	8-18	11	1
β-strand	21-32	12	1
β-strand	37-46	10	1
α-helix	48	1
α-helix	50	1
α-helix	54-60	7
β-strand	70	1	1
β-strand	87-95	9	1
β-strand	100-110	11	1
β-strand	113-123	11	1
β-strand	136-139	4	1
α-helix	140-141	2
β-strand	142-149	8	1
β-strand	152-163	12	1
β-strand	168-179	12	1
α-helix	184-189	6
β-strand	190-201	12	1
β-strand	207-217	11	1

Chain B: 6 helices, 13 β-strands

Element	Residues	Length	Sheet
β-strand	8-18	11	2
β-strand	21-32	12	2
β-strand	37-46	10	2
α-helix	48	1
α-helix	50	1
α-helix	54-60	7
β-strand	70	1	2
α-helix	78-81	4
β-strand	87-95	9	2
β-strand	100-110	11	2
β-strand	113-123	11	2
β-strand	136-139	4	2
α-helix	140-141	2
β-strand	142-149	8	2
β-strand	152-163	12	2
β-strand	168-179	12	2
α-helix	184-188	5
β-strand	190-201	12	2
β-strand	207-217	11	2

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Green to red photoconvertible GFP-like protein EosFP	A, B	protein	228	Lobophyllia hemprichii	Q5S6Z9 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>9J0R_1 Green to red photoconvertible GFP-like protein EosFP (chains A, B)
GPEFMSAIKPDMKIKLRMEGNVNGHHFVIDGEGTGKPFEGKQSMDLEVKEGGPLPFAFDI
LTTAFXNRVFAKYPDNIQDYFKQSFPKGYSWERSMTFEDGGICNARNDITMEGDTFYNKV
RFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLLEGGAHYRCDFRTTYK
AKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDNARR

Ligands and cofactors

ID	Name	Formula	Copies
DTT	2,3-dihydroxy-1,4-dithiobutane	C4 H10 O2 S2	1

Primary citation

Structural basis for the fast maturation of pcStar, a photoconvertible fluorescent protein. Zheng, S., Shi, X., Lin, J. et al. Acta Crystallogr D Struct Biol (2025) 81:181-195. DOI 10.1107/S2059798325002141 · PubMed

Other PDB entries of the same protein (UniProt Q5S6Z9 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7QLJ 1.02 Å, Trans structure of rsKiiro Illuminated at 290 K
7QLI 1.16 Å, Cis structure of rsKiiro at 290 K
7QLM 1.3 Å, rsKiiro trans chromophore dark structure by SFX
7QLO 1.31 Å, rsKiiro pump dump probe structure by TR-SFX
7QLL 1.32 Å, rsKiiro Thermal annealing at 290K of 200K Cis intermediate
9J0Q 1.34 Å, Structure of mEos3.2 in the green fluorescent state
7QLK 1.46 Å, Cis structure intermediate of rsKiiro Illuminated at 200 K
6GP0 1.5 Å, Structure of mEos4b in the red fluorescent state
7QLN 1.5 Å, rsKiiro pump probe structure by TR-SFX
6GP1 1.5 Å, Structure of mEos4b in the red long-lived dark state
5OQ9 1.51 Å, XFEL structure of the off state of a reversibly photoswitching fluorescent protein…
5OQA 1.53 Å, XFEL structure of the off state of a reversibly photoswitching fluorescent protein…

Browse structure collections

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