9V0U: GPR133-Gain-miniG13 complex

GPR133-Gain-miniG13 complex. Determined by electron microscopy at 3.51 Å resolution. Released 30 Jul 2025.

Method: Electron microscopy
Resolution: 3.51 Å
Organism: Homo sapiens
Chains: 4
Atoms: 6,205
Mol. weight: 138.04 kDa
Released: 30 Jul 2025

Explore 9V0U in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9V0U contains 34 α-helices and 37 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 11 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	11-32	22
β-strand	36-39	4	1
α-helix	42-44	3
α-helix	46-54	9
β-strand	69-70	2	1
β-strand	75	1	2
β-strand	80	1	2
β-strand	83-86	4	1
α-helix	94-97	4
α-helix	98-100	3
β-strand	105-111	7	1
α-helix	115-117	3
α-helix	118-129	12
β-strand	138-144	7	1
α-helix	148-152	5
α-helix	158-161	4
α-helix	172-186	15
β-strand	195-199	5	1
α-helix	207-227	21

Chain C: 2 helices, 29 β-strands

Element	Residues	Length	Sheet
α-helix	5-25	21
α-helix	30-33	4
β-strand	47	1	3
β-strand	51	1	3
β-strand	58-63	6	4
β-strand	69-74	6	4
β-strand	78-83	6	4
β-strand	88-94	7	4
β-strand	100-105	6	5
β-strand	111-116	6	5
β-strand	122-125	4	5
β-strand	134-138	5	5
β-strand	148-151	4	6
β-strand	157-160	4	6
β-strand	165-168	4	6
β-strand	178-181	4	6
β-strand	189-192	4	7
β-strand	198-202	5	7
β-strand	207-212	6	7
β-strand	218-223	6	7
β-strand	229-234	6	8
β-strand	240-245	6	8
β-strand	250-254	5	8
β-strand	259-264	6	8
β-strand	276-278	3	9
β-strand	284-289	6	9
β-strand	293-298	6	9
β-strand	307-309	3	9
β-strand	315-320	6	3
β-strand	327-331	5	3
β-strand	336-339	4	3

Chain G: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	9-23	15
α-helix	30-43	14
α-helix	53-55	3

Chain R: 18 helices, 0 β-strands

Element	Residues	Length
α-helix	547-550	4
α-helix	560-590	31
α-helix	602-624	23
α-helix	626	1
α-helix	630-661	32
α-helix	672-676	5
α-helix	677-681	5
α-helix	682-694	13
α-helix	695-697	3
α-helix	706-707	2
α-helix	714-739	26
α-helix	740-742	3
α-helix	757-769	13
α-helix	771-773	3
α-helix	774-777	4
α-helix	785-794	10
α-helix	798-806	9
α-helix	811-825	15

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Adhesion G-protein coupled receptor D1	R	protein	598	Homo sapiens	Q6QNK2 (AlphaFold model)
Guanine nucleotide-binding protein subunit alpha-13,Isoform 2 of Guanine nucleotide-binding…	A	protein	231	Homo sapiens	Q14344 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	C	protein	344	Homo sapiens	P62873 (AlphaFold model)

Sequence of entity 1 (R), FASTA

>9V0U_1 Adhesion G-protein coupled receptor D1 (chains R)
HPIITNLTEERKTFQSPGVILSYLQNVSLSLPSKSLSEQTALNLTKTFLKAVGEILLLPG
WIALSEDSAVVLSLIDTIDTVMGHVSSNLHGSTPQVTVEGSSAMAEFSVAKILPKTVNSS
HYRFPAHGQSFIQIPHEAFHRHAWSTVVGLLYHSMHYYLNNIWPAHTKIAEAMHHQDCLL
FATSHLISLEVSPPPTLSQNLSGSPLITVHLKHRLTRKQHSEATNSSNRVFVYCAFLDFS
SGEGVWSNHGCALTRGNLTYSVCRCTHLTNFAILMQVVPLELARGHQVALSSISYVGCSL
SVLCLVATLVTFAVLSSVSTIRNQRYHIHANLSFAVLVAQVLLLISFRLEPGTTPCQVMA
VLLHYFFLSAFAWMLVEGLHLYSMVIKVFGSEDSKHRYYYGMGWGFPLLICIISLSFAMD
SYGTSNNCWLSLASGAIWAFVAPALFVIVVNIGILIAVTRVISQISADNYKIHGDPSAFK
LTAKAVAVLLPILGTSWVFGVLAVNGCAVVFQYMFATLNSLQGLFIFLFHCLLNSEVRAA
FKHKTKVWSLTSSSARTSNAKPFHSDLMNGTRPGMASTKLSPWDKSSHSAHRVDLSAV

Sequence of entity 2 (A), FASTA

>9V0U_2 Guanine nucleotide-binding protein subunit alpha-13,Isoform 2 of Guanine nucleotide-binding protein subunit alpha-13 (chains A)
MMGSTVSAEDKAAAERSKEIDKCLSREKTYVKRLVKILLLGADNSGKSTFLKQMRIIHGG
SGGSGGTKGIHEYDFEIKNVPFKMVDVGGQRSERKRWFECFDSVTSILFLVDSSDFNRLT
ESLNDFETIVNNRVFSNVSIILFLNKTDLLEEKVQIVSIKDYFLEFEGDPHCLRDVQKFL
VECFRNKRRDQQQKPLYHHFTTAINTENARLIFRDVKDTILHDNLKQLMLQ

Sequence of entity 3 (G), FASTA

>9V0U_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 4 (C), FASTA

>9V0U_4 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains C)
GSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLA
KIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGG
LDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQ
TTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFP
NGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNV
WDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Primary citation

Cryo-EM structural elucidation and molecular mechanism of the GPR133-G13 signaling complex. Pu, X., Xi, Y.T., Wang, M.X. et al. Biochem Biophys Res Commun (2025) 777:152165-152165. DOI 10.1016/j.bbrc.2025.152165 · PubMed

Other PDB entries of the same protein (UniProt Q6QNK2 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8X9T 2.75 Å, Identification, structure and agonist design of an androgen membrane receptor
7WU2 2.8 Å, Cryo-EM structure of the adhesion GPCR ADGRD1 in complex with miniGs
8X9U 2.88 Å, Identification, structure and agonist design of an androgen membrane receptor
9IV1 2.98 Å, Identification, structure and agonist design of an androgen membrane receptor.
7EPT 3.0 Å, Structural basis for the tethered peptide activation of adhesion GPCRs
8X9S 3.49 Å, Identification, structure and agonist design of an androgen membrane receptor.
9IV2 3.53 Å, Identification, structure and agonist design of an androgen membrane receptor.

Browse structure collections

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