9XSG: HGZMA-hGSDMB

CryoEM structure of hGZMA-hGSDMB. Determined by electron microscopy at 3.4 Å resolution. Released 9 Sept 2026.

Method: Electron microscopy
Resolution: 3.4 Å
Organism: Homo sapiens
Chains: 4
Atoms: 7,564
Mol. weight: 148.97 kDa
Released: 9 Sept 2026

Explore 9XSG in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9XSG contains 37 α-helices and 58 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 5 helices, 24 β-strands

Element	Residues	Length	Sheet
β-strand	30	1	1
β-strand	33-34	2	2
α-helix	35-36	2
β-strand	43-47	5	3
β-strand	54-60	7	3
β-strand	63-67	5	3
β-strand	77-80	4	3
β-strand	84	1	4
β-strand	93-95	3	3
β-strand	97-102	6	3
β-strand	107	1	5
β-strand	112	1	5
β-strand	115-120	6	3
α-helix	123-125	3
α-helix	132-135	4
α-helix	141-143	3
β-strand	149	1	6
β-strand	150-152	3	2
β-strand	155	1	7
β-strand	162	1	7
β-strand	165	1	4
β-strand	167-169	3	2
β-strand	172-174	3	6
β-strand	196-199	4	6
β-strand	206	1	1
β-strand	215-218	4	6
β-strand	221-228	8	6
β-strand	242-245	4	6
α-helix	248-259	12

Chain B: 7 helices, 18 β-strands

Element	Residues	Length	Sheet
β-strand	30	1	8
β-strand	33-34	2	9
α-helix	35-36	2
β-strand	43-47	5	10
β-strand	54-60	7	10
β-strand	63-66	4	10
β-strand	77-80	4	10
β-strand	93-95	3	10
β-strand	97-102	6	10
β-strand	107	1	11
β-strand	112	1	11
β-strand	116-120	5	10
α-helix	123-124	2
α-helix	132-136	5
α-helix	141-143	3
β-strand	147-152	6	9
α-helix	166	1
β-strand	167-174	8	9
α-helix	176-179	4
β-strand	196-199	4	9
β-strand	206	1	8
β-strand	215-218	4	9
β-strand	221-228	8	9
β-strand	241-245	5	9
α-helix	248-259	12

Chain D: 10 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	256-263	8
α-helix	270-282	13
α-helix	287-303	17
α-helix	314-317	4
β-strand	320	1	16
β-strand	326	1	16
α-helix	328-343	16
α-helix	350-356	7
α-helix	360-373	14
α-helix	375-377	3
α-helix	382	1
α-helix	389-408	20

Chain E: 15 helices, 14 β-strands

Element	Residues	Length	Sheet
α-helix	2-16	15
β-strand	23-24	2	12
α-helix	29-31	3
β-strand	38-45	8	12
β-strand	48-57	10	12
α-helix	58-61	4
β-strand	62	1	13
β-strand	91	1	12
β-strand	120-124	5	12
α-helix	127-132	6
β-strand	136	1	14
β-strand	138	1	13
α-helix	144-147	4
β-strand	154-164	11	12
α-helix	179-182	4
β-strand	204-213	10	12
β-strand	220	1	14
β-strand	221-223	3	12
α-helix	254-267	14
α-helix	270-282	13
α-helix	288-303	16
α-helix	315-318	4
β-strand	320	1	15
β-strand	326	1	15
α-helix	328-341	14
α-helix	347-357	11
α-helix	360-371	12
α-helix	372-374	3
α-helix	389-408	20

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Granzyme A	A, B	protein	243	Homo sapiens	P12544 (AlphaFold model)
Gasdermin-B	D, E	protein	417	Homo sapiens	Q8TAX9 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>9XSG_1 Granzyme A (chains A, B)
IIGGNEVTPHSRPYMVLLSLDRKTICAGALIAKDWVLTAAHCNLNKRSQVILGAHSITRE
EPTKQIMLVKKEFPYPCYDPATREGDLKLLQLMEKAKINKYVTILHLPKKGDDVKPGTMC
QVAGWGRTHNSASWSDTLREVNITIIDRKVCNDRNHYNFNPVIGMNMVCAGSLRGGRDSC
NGDAGSPLLCEGVFRGVTSFGLENKCGDPRGPGVYILLSKKHLNWIIMTIKGAVGKTHHH
HHH

Sequence of entity 2 (D, E), FASTA

>9XSG_2 Gasdermin-B (chains D, E)
SMFSVFEEITRIVVKEMDAGGDMIAVRSLVDADRFRCFHLVGEKRTFFGCRHYTTGLTLM
DILDTDGDKWLDELDSGLQGQKAEFQILDNVDSTGELIVRLPKEITISGSFQGFHHQKIK
ISENRISQQYLATLENRKLKRELPFSFRSINTRENLYLVTETLETVKEETLKSDRQYKFW
SQISQGHLSYKHKGQREVTIPPNRVLSYRVKQLVFPNKETMNIHFRGKTKSFPEEKDGAS
SCLGKSLGSEDSRNMKEKLEDMESVLKDLTEEKRKDVLNSLAKCLGKEDIRQDLEQRVSE
VLISGELHMEDPDKPLLSSLFNAAGVLVEARAKAILDFLDALLELSEEQQFVAEALEKGT
LPLLKDQVKSVMEQNWDELASSPPDMDYDPEARILCALYVVVSILLELAEGPTSVSS

Primary citation

CryoEM structure of hGZMA-hGSDMB. Zhao, X., Zhang, K., Liu, Z. To be published.

Other PDB entries of the same protein (UniProt P12544 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1ORF 2.4 Å, The Oligomeric Structure of Human Granzyme A Reveals the Molecular Determinants of…
1OP8 2.5 Å, Crystal Structure of Human Granzyme A
9K1S 2.69 Å, Crystal structure of human granzyme A in complex with GSDMB-C domain
21AM 3.59 Å, CryoEM structure of hGZMA-hGSDMBctd

Browse structure collections

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