9YE8: Human muscle nAChR SCCMS eL269F ACh bound

Human muscle nAChR SCCMS eL269F ACh bound. Determined by electron microscopy at 2.22 Å resolution. Released 5 Aug 2026.

Method: Electron microscopy
Resolution: 2.22 Å
Organism: Homo sapiens
Chains: 5
Atoms: 16,613
Mol. weight: 268.33 kDa
Ligands: ACH, NAG
Released: 5 Aug 2026

Explore 9YE8 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9YE8 contains 81 α-helices and 83 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 16 helices, 14 β-strands

Element	Residues	Length	Sheet
α-helix	2-11	10
β-strand	29-44	16	1
β-strand	49-61	13	1
α-helix	69-72	4
β-strand	77-80	4	1
α-helix	82-84	3
β-strand	90-92	3	2
β-strand	95	1	1
β-strand	101	1	2
α-helix	106-107	2
β-strand	108-111	4	1
β-strand	115-118	4	1
β-strand	121-127	7	1
β-strand	139-148	10	2
β-strand	156-160	5	1
β-strand	166	1	1
α-helix	170-173	4
β-strand	176-190	15	2
β-strand	193-209	17	2
α-helix	212-214	3
α-helix	215-219	5
α-helix	220-230	11
α-helix	231-233	3
α-helix	237-239	3
α-helix	242-261	20
α-helix	273-299	27
α-helix	310-313	4
α-helix	314-319	6
α-helix	320-323	4
α-helix	370-436	67

Chain B: 15 helices, 19 β-strands

Element	Residues	Length	Sheet
α-helix	3-12	10
β-strand	29-44	16	12
β-strand	49-61	13	12
α-helix	63-65	3
α-helix	69-72	4
β-strand	77-80	4	12
α-helix	82-84	3
β-strand	90-92	3	13
β-strand	95	1	12
α-helix	106-107	2
β-strand	108-111	4	12
β-strand	115-118	4	12
β-strand	121-127	7	12
β-strand	139-148	10	13
β-strand	156-160	5	12
β-strand	162	1	14
β-strand	168	1	14
β-strand	171	1	15
β-strand	173	1	12
α-helix	176-178	3
β-strand	180	1	12
β-strand	185-189	5	13
β-strand	191	1	15
β-strand	192-197	6	13
β-strand	207-218	12	13
α-helix	221-223	3
α-helix	224-228	5
α-helix	229-236	8
α-helix	237-243	7
α-helix	252-271	20
α-helix	283-309	27
α-helix	320-324	5
α-helix	325-329	5
α-helix	400-458	59

Chain C: 18 helices, 14 β-strands

Element	Residues	Length	Sheet
α-helix	2-11	10
α-helix	28	1
β-strand	29-44	16	3
β-strand	49-61	13	3
α-helix	69-72	4
β-strand	77-81	5	3
α-helix	82-84	3
β-strand	90-92	3	4
β-strand	95	1	3
β-strand	101	1	4
α-helix	106	1
β-strand	107-111	5	3
β-strand	115-118	4	3
β-strand	121-127	7	3
β-strand	139-148	10	4
β-strand	156-160	5	3
β-strand	166	1	3
α-helix	170-173	4
β-strand	176-190	15	4
β-strand	193-209	17	4
α-helix	212-214	3
α-helix	215-219	5
α-helix	220-229	10
α-helix	230-232	3
α-helix	242-262	21
α-helix	273-299	27
α-helix	310-313	4
α-helix	314-319	6
α-helix	320-323	4
α-helix	370-423	54
α-helix	424-428	5
α-helix	429-434	6

Chain D: 18 helices, 19 β-strands

Element	Residues	Length	Sheet
α-helix	2-8	7
α-helix	9-14	6
β-strand	31-46	16	8
β-strand	51-68	18	8
α-helix	71-74	4
β-strand	79-82	4	8
α-helix	84-86	3
β-strand	93-94	2	9
β-strand	97	1	8
α-helix	108-109	2
β-strand	110-113	4	8
β-strand	115-120	6	8
β-strand	123-129	7	8
β-strand	141-149	9	9
β-strand	158-162	5	8
β-strand	164-167	4	10
β-strand	170-173	4	10
β-strand	176	1	11
β-strand	178	1	8
β-strand	185	1	8
β-strand	190-194	5	9
β-strand	196	1	11
β-strand	197-201	5	9
α-helix	210-212	3
β-strand	213-223	11	9
α-helix	226-228	3
α-helix	229-233	5
α-helix	234-242	9
α-helix	243-248	6
α-helix	251-253	3
α-helix	256-275	20
α-helix	287-313	27
α-helix	324-328	5
α-helix	329-333	5
α-helix	334-337	4
α-helix	411-414	4
α-helix	415-472	58

Chain E: 14 helices, 17 β-strands

Element	Residues	Length	Sheet
α-helix	2-11	10
β-strand	29-44	16	5
β-strand	49-66	18	5
α-helix	69-72	4
β-strand	77-81	5	5
β-strand	90-92	3	6
β-strand	95	1	5
β-strand	107-111	5	5
β-strand	113-118	6	5
β-strand	121-127	7	5
β-strand	139-148	10	6
β-strand	156-160	5	5
β-strand	171	1	7
β-strand	173	1	5
β-strand	180	1	5
β-strand	185-189	5	6
β-strand	191	1	7
β-strand	192-195	4	6
α-helix	196-198	3
β-strand	211-220	10	6
α-helix	223-225	3
α-helix	226-230	5
α-helix	231-242	12
α-helix	243-245	3
α-helix	248-250	3
α-helix	253-272	20
α-helix	284-310	27
α-helix	321-324	4
α-helix	325-330	6
α-helix	331-334	4
α-helix	408-469	62

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Acetylcholine receptor subunit alpha	A, C	protein	437	Homo sapiens	P02708 (AlphaFold model)
Acetylcholine receptor subunit beta	E	protein	480	Homo sapiens	P11230 (AlphaFold model)
Acetylcholine receptor subunit delta	D	protein	496	Homo sapiens	Q07001 (AlphaFold model)
Acetylcholine receptor subunit epsilon	B	protein	473	Homo sapiens	Q04844 (AlphaFold model)

Sequence of entity 1 (A, C), FASTA

>9YE8_1 Acetylcholine receptor subunit alpha (chains A, C)
SEHETRLVAKLFKDYSSVVRPVEDHRQVVEVTVGLQLIQLINVDEVNQIVTTNVRLKQQW
VDYNLKWNPDDYGGVKKIHIPSEKIWRPDLVLYNNADGDFAIVKFTKVLLQYTGHITWTP
PAIFKSYCEIIVTHFPFDEQNCSMKLGTWTYDGSVVAINPESDQPDLSNFMESGEWVIKE
SRGWKHSVTYSCCPDTPYLDITYHFVMQRLPLYFIVNVIIPCLLFSFLTGLVFYLPTDSG
EKMTLSISVLLSLTVFLLVIVELIPSTSSAVPLIGKYMLFTMVFVIASIIITVIVINTHH
RSPSTHVMPNWVRKVFIDTIPNIMFFSTMKRPSREKQDKKIFTEDIDISDISGKPGPPPM
GFHSPLIKHPEVKSAIEGIKYIAETMKSDQESNNAAAEWKYVAMVMDHILLGVFMLVCII
GTLAVFAGRLIELNQQG

Sequence of entity 2 (E), FASTA

>9YE8_2 Acetylcholine receptor subunit beta (chains E)
SEAEGRLREKLFSGYDSSVRPAREVGDRVRVSVGLILAQLISLNEKDEEMSTKVYLDLEW
TDYRLSWDPAEHDGIDSLRITAESVWLPDVVLLNNNDGNFDVALDISVVVSSDGSVRWQP
PGIYRSSCSIQVTYFPFDWQNCTMVFSSYSYDSSEVSLQTGLGPDGQGHQEIHIHEGTFI
ENGQWEIIHKPSRLIQPPGDPRGGREGQRQEVIFYLIIRRKPLFYLVNVIAPCILITLLA
IFVFYLPPDAGEKMGLSIFALLTLTVFLLLLADKVPETSLSVPIIIKYLMFTMVLVTFSV
ILSVVVLNLHHRSPHTHQMPLWVRQIFIHKLPLYLRLKRPKPERDLMPEPPHCSSPGSGW
GRGTDEYFIRKPPSDFLFPKPNRFQPELSAPDLRRFIDGPNRAVALLPELREVVSSISYI
ARQLQEQEDHDALKEDWQFVAMVVDRLFLWTFIIFTSVGTLVIFLDATYHLPPPDPFPSR

Sequence of entity 3 (D), FASTA

>9YE8_3 Acetylcholine receptor subunit delta (chains D)
LNEEERLIRHLFQEKGYNKELRPVAHKEESVDVALALTLSNLISLKEVEETLTTNVWIEH
GWTDNRLKWNAEEFGNISVLRLPPDMVWLPEIVLENNNDGSFQISYSCNVLVYHYGFVYW
LPPAIFRSSCPISVTYFPFDWQNCSLKFSSLKYTAKEITLSLKQDAKENRTYPVEWIIID
PEGFTENGEWEIVHRPARVNVDPRAPLDSPSRQDITFYLIIRRKPLFYIINILVPCVLIS
FMVNLVFYLPADSGEKTSVAISVLLAQSVFLLLISKRLPATSMAIPLIGKFLLFGMVLVT
MVVVICVIVLNIHFRTPSTHVLSEGVKKLFLETLPELLHMSRPAEDGPSPGALVRRSSSL
GYISKAEEYFLLKSRSDLMFEKQSERHGLARRLTTARRPPASSEQAQQELFNELKPAVDG
ANFIVNHMRDQNNYNEEKDSWNRVARTVDRLCLFVVTPVMVVGTAWIFLQGVYNQPPPQP
FPGDPYSYNVQDKRFI

Sequence of entity 4 (B), FASTA

>9YE8_4 Acetylcholine receptor subunit epsilon (chains B)
KNEELRLYHHLFNNYDPGSRPVREPEDTVTISLKVTLTNLISLNEKEETLTTSVWIGIDW
QDYRLNYSKDDFGGIETLRVPSELVWLPEIVLENNIDGQFGVAYDANVLVYEGGSVTWLP
PAIYRSVCAVEVTYFPFDWQNCSLIFRSQTYNAEEVEFTFAVDNDGKTINKIDIDTEAYT
ENGEWAIDFCPGVIRRHHGGATDGPGETDVIYSLIIRRKPLFYVINIIVPCVLISGLVLL
AYFLPAQAGGQKCTVSINVLLAQTVFLFFIAQKIPETSLSVPLLGRFLIFVMVVATLIVM
NCVIVLNVSQRTPTTHAMSPRLRHVLLELLPRLLGSPPPPEAPRAASPPRRASSVGLLLR
AEELILKKPRSELVFEGQRHRQGTWTAAFCQSLGAAAPEVRCCVDAVNFVAESTRDQEAT
GEEVSDWVRMGNALDNICFWAALVLFSVGSSLIFLGAYFNRVPDLPYAPCIQP

Ligands and cofactors

ID	Name	Formula	Copies
ACH	Acetylcholine	C7 H16 N O2	2
NAG	2-acetamido-2-deoxy-beta-D-glucopyranose	C8 H15 N O6	1

Primary citation

Correcting congenital myasthenia-associated acetylcholine receptor defects. Li, H., Mukhtasimova, N., Teng, J. et al. Nature (2026) 656:516-524. DOI 10.1038/s41586-026-10706-1 · PubMed

Other PDB entries of the same protein (UniProt P02708 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9DMS 1.92 Å, Human muscle nAChR with fab6-bound
9DMG 2.05 Å, Human muscle nAChR apo state
9DMH 2.06 Å, Human muscle nAChR ACh-bound state
9DMQ 2.06 Å, Human muscle nAChR with fab3-bound
9DMV 2.13 Å, Human muscle nAChR with fab9-bound
9DMT 2.18 Å, Human muscle nAChR with fab7-bound
9DMJ 2.19 Å, Human muscle nAChR with two fab1b-bound
9YER 2.19 Å, Human muscle nAChR SCCMS eT264P ACh bound
4ZJS 2.23 Å, Crystal structure of a chimeric acetylcholine binding protein from Aplysia Californica…
9DML 2.24 Å, Human muscle nAChR with fab2-bound
9YEI 2.36 Å, Human muscle nAChR SCCMS eL269F ACh, Reboxetine bound (lower 1)
9YET 2.36 Å, Human muscle nAChR SCCMS eT264P ACh, Reboxetine bound

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