4FZD: MST4-MO25 complex with WSF motif

Crystal structure of MST4-MO25 complex with WSF motif. Determined by X-ray diffraction at 3.25 Å resolution. Released 6 Mar 2013.

Method: X-ray diffraction
Resolution: 3.25 Å
Organism: Homo sapiens
Chains: 3
Atoms: 4,597
Mol. weight: 71 kDa
Released: 6 Mar 2013

Explore 4FZD in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

4FZD contains 41 α-helices and 11 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 25 helices, 0 β-strands

Element	Residues	Length
α-helix	12-26	15
α-helix	34-37	4
α-helix	39-53	15
α-helix	66-75	10
α-helix	79-85	7
α-helix	87-89	3
α-helix	92-107	16
α-helix	115-122	8
α-helix	126-132	7
α-helix	133-135	3
α-helix	140-150	11
α-helix	154-161	8
α-helix	164-168	5
α-helix	169-172	4
α-helix	178-191	14
α-helix	196-203	8
α-helix	207-218	12
α-helix	223-237	15
α-helix	243-249	7
α-helix	253-262	10
α-helix	268-282	15
α-helix	289-297	9
α-helix	299-308	10
α-helix	314-316	3
α-helix	318-331	14

Chain B: 16 helices, 11 β-strands

Element	Residues	Length	Sheet
α-helix	20-23	4
β-strand	24-29	6	1
β-strand	38-43	6	1
β-strand	49-55	7	1
α-helix	64-75	12
β-strand	82	1	2
α-helix	83-84	2
β-strand	85-91	7	1
β-strand	94-100	7	1
β-strand	105-106	2	2
α-helix	107-111	5
α-helix	118-137	20
β-strand	140-141	2	3
α-helix	147-149	3
β-strand	150-152	3	2
β-strand	158-160	3	2
β-strand	166-167	2	3
α-helix	188-191	4
α-helix	199-214	16
α-helix	224-233	10
α-helix	235-237	3
α-helix	245-254	10
α-helix	259-261	3
α-helix	265-268	4
α-helix	272-277	6
α-helix	281-284	4
α-helix	285-294	10

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Calcium-binding protein 39	A	protein	328	Homo sapiens	Q9Y376 (AlphaFold model)
Serine/threonine-protein kinase MST4	B	protein	283	Homo sapiens	Q9P289 (AlphaFold model)
C-terminal peptide from Serine/threonine-protein kinase MST4	C	protein	5	Homo sapiens	Q9P289 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>4FZD_1 Calcium-binding protein 39 (chains A)
GAMASPADIVKNLKESMAVLEKQDISDKKAEKATEEVSKNLVAMKEILYGTNEKEPQTEA
VAQLAQELYNSGLLSTLVADLQLIDFEGKKDVAQIFNNILRRQIGTRTPTVEYICTQQNI
LFMLLKGYESPEIALNCGIMLRECIRHEPLAKIILWSEQFYDFFRYVEMSTFDIASDAFA
TFKDLLTRHKLLSAEFLEQHYDRFFSEYEKLLHSENYVTKRQSLKLLGELLLDRHNFTIM
TKYISKPENLKLMMNLLRDKSRNIQFEAFHVFKVFVANPNKTQPILDILLKNQAKLIEFL
SKFQNDRTEDEQFNDEKTYLVKQIRDLK

Sequence of entity 2 (B), FASTA

>4FZD_2 Serine/threonine-protein kinase MST4 (chains B)
GAMADPEELFTKLERIGKGSFGEVFKGIDNRTQQVVAIKIIDLEEAEDEIEDIQQEITVL
SQCDSSYVTKYYGSYLKGSKLWIIMEYLGGGSALDLLRAGPFDEFQIATMLKEILKGLDY
LHSEKKIHRDIKAANVLLSEQGDVKLAAFGVAGQLTDTQIKRNTFVGTPFWMAPEVIQQS
AYDSKADIWSLGITAIELAKGEPPNSDMHPMRVLFLIPKNNPPTLVGDFTKSFKEFIDAC
LNKDPSFRPTAKELLKHKFIVKNSKKTSYLTELIDRFKRWKAE

Sequence of entity 3 (C), FASTA

>4FZD_3 C-terminal peptide from Serine/threonine-protein kinase MST4 (chains C)
EWSFT

Primary citation

Structure of the MST4 in Complex with MO25 Provides Insights into Its Activation Mechanism. Shi, Z., Jiao, S., Zhang, Z. et al. Structure (2013) 21:449-461. DOI 10.1016/j.str.2013.01.007 · PubMed

Other PDB entries of the same protein (UniProt Q9Y376 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1UPK 1.85 Å, Crystal structure of MO25 in complex with a C-terminal peptide of STRAD
3GNI 2.35 Å, Structure of STRAD and MO25
1UPL 2.6 Å, Crystal structure of MO25 alpha
2WTK 2.65 Å, Structure of the heterotrimeric LKB1-STRADalpha-MO25alpha complex
8VSU 2.86 Å, Cryo-EM structure of LKB1-STRADalpha-MO25alpha heterocomplex
4FZA 3.15 Å, Crystal structure of MST4-MO25 complex
4O27 3.19 Å, Crystal structure of MST3-MO25 complex with WIF motif
4NZW 3.58 Å, Crystal Structure of STK25-MO25 Complex
4FZF 3.64 Å, Crystal structure of MST4-MO25 complex with DKI

Browse structure collections

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