7T10: Somatostatin receptor 2

CryoEM structure of somatostatin receptor 2 in complex with somatostatin-14 and Gi3. Determined by electron microscopy at 2.5 Å resolution. Released 9 Mar 2022.

Method: Electron microscopy
Resolution: 2.5 Å
Organisms: Homo sapiens, Mus musculus
Chains: 6
Atoms: 8,429
Mol. weight: 161.33 kDa
Released: 9 Mar 2022

Explore 7T10 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7T10 contains 37 α-helices and 62 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 9 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	7-31	25
β-strand	34-40	7	2
α-helix	46-52	7
β-strand	185-191	7	2
β-strand	194-200	7	2
α-helix	208-211	4
α-helix	212-215	4
β-strand	220-226	7	2
α-helix	242-254	13
β-strand	263-269	7	2
α-helix	271-280	10
α-helix	283-285	3
α-helix	296-309	14
β-strand	319-323	5	2
α-helix	330-351	22

Chain B: 2 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	4-25	22
α-helix	30-33	4
β-strand	47-51	5	3
β-strand	58-63	6	4
β-strand	69-74	6	4
β-strand	78-83	6	4
β-strand	89-94	6	4
β-strand	100-105	6	5
β-strand	111-116	6	5
β-strand	121-125	5	5
β-strand	134-139	6	5
β-strand	146-151	6	6
β-strand	156-161	6	6
β-strand	166-170	5	6
β-strand	175-180	6	6
β-strand	187-192	6	7
β-strand	198-203	6	7
β-strand	207-212	6	7
β-strand	218-223	6	7
β-strand	229-234	6	8
β-strand	240-245	6	8
β-strand	250-254	5	8
β-strand	259-264	6	8
β-strand	273-278	6	9
β-strand	284-289	6	9
β-strand	294-298	5	9
β-strand	304-308	5	9
β-strand	315-320	6	3
β-strand	327-331	5	3
β-strand	336-339	4	3

Chain C: 5 helices, 0 β-strands

Element	Residues	Length
α-helix	8-23	16
α-helix	30-42	13
α-helix	45-47	3
α-helix	53-55	3
α-helix	56-58	3

Chain P: 0 helices, 2 β-strands

Element	Residues	Length	Sheet
β-strand	6-7	2	10
β-strand	10-11	2	10

Chain R: 16 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	41-69	29
α-helix	77-92	16
α-helix	96-104	9
α-helix	112-145	34
α-helix	149-151	3
α-helix	156-173	18
α-helix	175-179	5
β-strand	181-185	5	1
β-strand	191-195	5	1
α-helix	198-200	3
α-helix	204-213	10
α-helix	214-218	5
α-helix	219-237	19
α-helix	245-281	37
α-helix	288-300	13
α-helix	303-309	7
α-helix	310-314	5
α-helix	320-326	7

Chain S: 5 helices, 24 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	11
β-strand	10-12	3	12
β-strand	18-25	8	11
α-helix	29-31	3
β-strand	33-39	7	12
β-strand	45-51	7	12
β-strand	58-60	3	12
β-strand	68-73	6	11
β-strand	78-83	6	11
α-helix	88-90	3
β-strand	92-99	8	12
β-strand	110-111	2	12
β-strand	115-119	5	12
β-strand	128-129	2	13
β-strand	135	1	14
β-strand	143-149	7	13
β-strand	154	1	15
β-strand	160	1	15
β-strand	162-167	6	14
β-strand	174-178	5	14
β-strand	182-183	2	14
α-helix	184	1
β-strand	191-195	5	13
β-strand	199-204	6	13
α-helix	209-211	3
β-strand	213-219	7	14
α-helix	225	1
β-strand	227	1	14
β-strand	231-233	3	14

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Somatostatin receptor type 2,Kappa-type opioid receptor	R	protein	408	Homo sapiens	P30874 (AlphaFold model), P41145 (AlphaFold model)
Guanine nucleotide-binding protein G(i) subunit alpha-3	A	protein	354	Homo sapiens	P08754 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	344	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	C	protein	71	Homo sapiens	P59768
Somatostatin-14	P	protein	14	Homo sapiens	P61278
scFv16	S	protein	259	Mus musculus

Sequence of entity 1 (R), FASTA

>7T10_1 Somatostatin receptor type 2,Kappa-type opioid receptor (chains R)
DYKDDDDAMGQPGNGSAFLLAPNRSHAPDHDVENLYFQGMDMADEPLNGSHTWLSIPFDL
NGSVVSTNTSNQTEPYYDLTSNAVLTFIYFVVCIIGLCGNTLVIYVILRYAKMKTITNIY
ILNLAIADELFMLGLPFLAMQVALVHWPFGKAICRVVMTVDGINQFTSIFCLTVMSIDRY
LAVVHPIKSAKWRRPRTAKMITMAVWGVSLLVILPIMIYAGLRSNQWGRSSCTINWPGES
GAWYTGFIIYTFILGFLVPLTIICLCYLFIIIKVKSVRLLSGSREKDRNLRKVTRMVSIV
VAVFIFCWLPFYIFNVSSVSMAISPTPALKGMFDFVVVLTYANSCANPILYAFLSDNFKK
SFQNVLCLVKVSGTDDGERSDSKQDKSRLNETTETQRTLLNGDLQTSI

Sequence of entity 2 (A), FASTA

>7T10_2 Guanine nucleotide-binding protein G(i) subunit alpha-3 (chains A)
MGCTLSAEDKAAVERSKMIDRNLREDGEKAAKEVKLLLLGAGESGKNTIVKQMKIIHEDG
YSEDECKQYKVVVYSNTIQSIIAIIRAMGRLKIDFGEAARADDARQLFVLAGSAEEGVMT
PELAGVIKRLWRDGGVQACFSRSREYQLNDSASYYLNDLDRISQSNYIPTQQDVLRTRVK
TTGIVETHFTFKDLYFKMFDVGAQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEM
NRMHASMKLFDSICNNKWFTETSIILFLNKKDLFEEKIKRSPLTICYPEYTGSNTYEEAA
AYIQCQFEDLNRRKDTKEIYTHFTCSTDTKNVQFVFDAVTDVIIKNNLKECGLY

Sequence of entity 3 (B), FASTA

>7T10_3 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
PGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLA
KIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGG
LDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQ
TTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFP
NGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNV
WDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 4 (C), FASTA

>7T10_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains C)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 5 (P), FASTA

>7T10_5 Somatostatin-14 (chains P)
AGCKNFFWKTFTSC

Sequence of entity 6 (S), FASTA

>7T10_6 scFv16 (chains S)
DVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYY
ADTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVS
SGGGGSGGGGSGGGGSDIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLELKAAAHHHHHHHH

Primary citation

Plasticity in ligand recognition at somatostatin receptors. Robertson, M.J., Meyerowitz, J.G., Panova, O. et al. Nat Struct Mol Biol (2022) 29:210-217. DOI 10.1038/s41594-022-00727-5 · PubMed

Other PDB entries of the same protein (UniProt P30874 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7XN9 2.6 Å, Crystal structure of SSTR2 and L-054,522 complex
7XNA 2.65 Å, Crystal structure of somatostatin receptor 2 (SSTR2) with peptide antagonist CYN 154806
7T11 2.7 Å, CryoEM structure of somatostatin receptor 2 in complex with Octreotide and Gi3.
7WIG 2.7 Å, Cryo-EM structure of the L-054,264-bound human SSTR2-Gi1 complex
7WIC 2.8 Å, Cryo-EM structure of the SS-14-bound human SSTR2-Gi1 complex
7XAT 2.85 Å, Structure of somatostatin receptor 2 bound with SST14.
7XAV 2.87 Å, Structure of somatostatin receptor 2 bound with lanreotide.
7XAU 2.97 Å, Structure of somatostatin receptor 2 bound with octreotide.
7UL5 3.1 Å, CryoEM Structure of Inactive SSTR2 bound to Nb6
7XMR 3.1 Å, CryoEM structure of the somatostatin receptor 2 (SSTR2) in complex with Gi1 and its…
7YAC 3.24 Å, Paltusotine-bound SSTR2-Gi complex
7Y24 3.25 Å, Cryo-EM structure of the octreotide-bound SSTR2-miniGo-scFv16 complex

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