7TYX: Human Amylin2 Receptor

Human Amylin2 Receptor in complex with Gs and rat amylin peptide. Determined by electron microscopy at 2.55 Å resolution. Released 30 Mar 2022.

Method: Electron microscopy
Resolution: 2.55 Å
Organisms: Homo sapiens, Rattus norvegicus, Lama glama
Chains: 7
Atoms: 10,219
Mol. weight: 191.14 kDa
Ligands: NAG, PLM, Y01
Released: 30 Mar 2022

Explore 7TYX in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7TYX contains 51 α-helices and 58 β-strands across 7 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 11 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	12-39	28
β-strand	41-47	7	7
α-helix	53-59	7
β-strand	207-214	8	7
β-strand	217-224	8	7
α-helix	236-238	3
β-strand	243-249	7	7
α-helix	265-277	13
α-helix	280-282	3
β-strand	286-292	7	7
α-helix	294-302	9
α-helix	308-311	4
α-helix	313-316	4
α-helix	326-327	2
α-helix	332-351	20
β-strand	359-363	5	7
α-helix	371-390	20

Chain B: 4 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	3-25	23
α-helix	30-33	4
α-helix	38-39	2
β-strand	47-51	5	8
β-strand	58-63	6	9
β-strand	69-74	6	9
β-strand	78-83	6	9
β-strand	88-94	7	9
α-helix	95	1
β-strand	100-105	6	10
β-strand	111-116	6	10
β-strand	121-125	5	10
β-strand	134-139	6	10
β-strand	146-151	6	11
β-strand	156-161	6	11
β-strand	165-170	6	11
β-strand	175-181	7	11
β-strand	187-192	6	12
β-strand	198-203	6	12
β-strand	208-212	5	12
β-strand	218-222	5	12
β-strand	229-234	6	13
β-strand	240-245	6	13
β-strand	250-254	5	13
β-strand	259-264	6	13
β-strand	273-278	6	14
β-strand	284-289	6	14
β-strand	294-298	5	14
β-strand	304-308	5	14
β-strand	317-320	4	8
β-strand	327-330	4	8
β-strand	336-339	4	8

Chain E: 6 helices, 1 β-strand

Element	Residues	Length	Sheet
α-helix	61-76	16
α-helix	81-83	3
α-helix	86-106	21
α-helix	114-126	13
α-helix	140-142	3
α-helix	143-166	24
β-strand	169	1	1

Chain G: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	9-23	15
α-helix	30-43	14
α-helix	53-55	3

Chain N: 3 helices, 11 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	15
β-strand	10-12	3	16
β-strand	18-25	8	15
α-helix	29-31	3
β-strand	34-39	6	16
β-strand	45-51	7	16
β-strand	58-60	3	16
α-helix	62-64	3
β-strand	68-73	6	15
β-strand	78-83	6	15
α-helix	88-90	3
β-strand	92-98	7	16
β-strand	118	1	16
β-strand	122-126	5	16

Chain P: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	8-19	12
α-helix	27-31	5

Chain R: 22 helices, 12 β-strands

Element	Residues	Length	Sheet
α-helix	40-60	21
β-strand	71-72	2	2
β-strand	75-76	2	3
β-strand	81-82	2	3
β-strand	85-86	2	2
β-strand	91-94	4	4
α-helix	95-96	2
β-strand	107-110	4	4
β-strand	112	1	5
β-strand	118	1	5
β-strand	120-121	2	6
β-strand	126-127	2	6
β-strand	130	1	4
α-helix	132-135	4
α-helix	138-172	35
α-helix	174-176	3
α-helix	179-200	22
α-helix	201-205	5
α-helix	209-214	6
α-helix	217-245	29
α-helix	246-250	5
β-strand	257	1	1
α-helix	260-263	4
α-helix	264-268	5
α-helix	271-283	13
α-helix	288-290	3
α-helix	296-298	3
α-helix	299-330	32
α-helix	334-352	19
α-helix	355-358	4
α-helix	366-381	16
α-helix	383-388	6
α-helix	389-393	5
α-helix	396-404	9

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Receptor activity-modifying protein 2	E	protein	156	Homo sapiens	O60895 (AlphaFold model)
amylin peptide	P	protein	38	Rattus norvegicus	P12969 (AlphaFold model)
Calcitonin receptor	R	protein	501	Homo sapiens	P30988 (AlphaFold model)
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	A	protein	394	Homo sapiens	P63092 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	350	Homo sapiens	P62873
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768
nanobody 35	N	protein	138	Lama glama

Sequence of entity 1 (E), FASTA

>7TYX_1 Receptor activity-modifying protein 2 (chains E)
MKTIIALSYIFCLVFADYKDDDDKPLPTTGTPGSEGGTVKNYETAVQFCWNHYKDQMDPI
EKDWCDWAMISRPYSTLRDCLEHFAELFDLGFPNPLAERIIFETHQIHFANCSLVQPTFS
DPPEDVLLAMIIAPICLIPFLITLVVWRSKDSEAQA

Sequence of entity 2 (P), FASTA

>7TYX_2 amylin peptide (chains P)
KCNTATCATQRLANFLVRSSNNLGPVLPPTNVGSNTYX

Sequence of entity 3 (R), FASTA

>7TYX_3 Calcitonin receptor (chains R)
MKTIIALSYIFCLVFADYKDDDDLEVLFQGPAAFSNQTYPTIEPKPFLYVVGRKKMMDAQ
YKCYDRMQQLPAYQGEGPYCNRTWDGWLCWDDTPAGVLSYQFCPDYFPDFDPSEKVTKYC
DEKGVWFKHPENNRTWSNYTMCNAFTPEKLKNAYVLYYLAIVGHSLSIFTLVISLGIFVF
FRSLGCQRVTLHKNMFLTYILNSMIIIIHLVEVVPNGELVRRDPVSCKILHFFHQYMMAC
NYFWMLCEGIYLHTLIVVAVFTEKQRLRWYYLLGWGFPLVPTTIHAITRAVYFNDNCWLS
VETHLLYIIHGPVMAALVVNFFFLLNIVRVLVTKMRETHEAESHMYLKAVKATMILVPLL
GIQFVVFPWRPSNKMLGKIYDYVMHSLIHFQGFFVATIYCFCNNEVQTTVKRQWAQFKIQ
WNQRWGRRPSNRSARAAAAAAEAGDIPIYICHQELRNEPANNQGEESAEIIPLNIIEQES
SAPAGLEVLFQGPHHHHHHHH

Sequence of entity 4 (A), FASTA

>7TYX_4 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKNTIVKQM
RILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELA
NPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLD
KIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFND
VTAIIFVVASSSYNMVIREDNQTNRLQAALKLFDSIWNNKWLRDTSVILFLNKQDLLAEK
VLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCY
PHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

Sequence of entity 5 (B), FASTA

>7TYX_5 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRT
LRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGN
YVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWD
IETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDIN
AICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYD
DFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 6 (G), FASTA

>7TYX_6 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 7 (N), FASTA

>7TYX_7 nanobody 35 (chains N)
QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISY
TGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQ
GTQVTVSSHHHHHHEPEA

Ligands and cofactors

ID	Name	Formula	Copies
NAG	2-acetamido-2-deoxy-beta-D-glucopyranose	C8 H15 N O6	3
PLM	Palmitic acid	C16 H32 O2	8
Y01	Cholesterol hemisuccinate	C31 H50 O4	2

Primary citation

A structural basis for amylin receptor phenotype. Cao, J., Belousoff, M.J., Liang, Y.L. et al. Science (2022) 375:eabm9609-eabm9609. DOI 10.1126/science.abm9609 · PubMed

Other PDB entries of the same protein (UniProt O60895 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4RWF 1.76 Å, Crystal structure of the CLR:RAMP2 extracellular domain heterodimer with bound…
6V2E 1.83 Å, Crystal structure of the human CLR:RAMP2 extracellular domain heterodimer with bound…
3AQE 2.0 Å, Crystal structure of the extracellular domain of human RAMP2
2XVT 2.05 Å, Structure of the extracellular domain of human RAMP2
3AQF 2.6 Å, Crystal structure of the human CRLR/RAMP2 extracellular complex
9BQ3 2.8 Å, Human Amylin2 Receptor in Complex with Gs and Cagrilintide
6UUN 3.0 Å, CryoEM Structure of the active Adrenomedullin 1 receptor G protein complex with…
7TYY 3.0 Å, Human Amylin2 Receptor in complex with Gs and salmon calcitonin peptide
7TYH 3.3 Å, Human Amylin2 Receptor in complex with Gs and human calcitonin peptide

Browse structure collections

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