7WLT: The Curved Structure of mPIEZO1 in Lipid Bilayer

the Curved Structure of mPIEZO1 in Lipid Bilayer. Determined by electron microscopy at 3.46 Å resolution. Released 13 Apr 2022.

Method
Electron microscopy
Resolution
3.46 Å
Organism
Mus musculus
Chains
3
Atoms
33,912
Mol. weight
897.68 kDa
Ligands
P5S, PEE, PLX
Released
13 Apr 2022

Explore 7WLT in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7WLT contains 183 α-helices and 48 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chains A, C and E: 61 helices, 16 β-strands

ElementResiduesLengthSheet
α-helix785-82339
α-helix827-83913
α-helix846-86722
α-helix925-94925
α-helix974-98411
α-helix990-100516
α-helix1008-102215
α-helix1026-105227
α-helix1074-10796
α-helix1094-111320
α-helix1149-115911
α-helix1162-117514
α-helix1181-120020
α-helix1203-122725
α-helix1228-12314
α-helix1243-12497
α-helix1264-12663
α-helix1281-129818
α-helix1300-136465
α-helix1403-14075
α-helix1412-14154
α-helix1494-154754
α-helix1553-15564
α-helix1657-166812
α-helix1673-168513
α-helix1687-170014
α-helix1705-17139
α-helix1714-17185
α-helix1727-174822
α-helix1759-17624
α-helix1770-17745
α-helix1787-180216
α-helix1947-196721
α-helix1977-199620
α-helix2017-204024
α-helix2043-205917
α-helix2060-20645
α-helix2065-20684
α-helix2077-209923
α-helix2101-21022
α-helix2109-21113
α-helix2116-212712
α-helix2131-214212
α-helix2149-217426
α-helix2182-21843
α-helix2185-221329
β-strand221911
β-strand2223-2232102
β-strand2236-224382
β-strand2248-225033
α-helix2251-22522
α-helix2253-226210
α-helix2267-22759
α-helix2278-22803
β-strand2281-228443
β-strand2286-228724
α-helix2298-230912
β-strand2315-2325112
β-strand2335-2344102
α-helix2349-23579
β-strand2366-237275
β-strand2375-237843
α-helix23851
β-strand238613
α-helix23871
β-strand2399-2409115
α-helix2423-24253
β-strand2426-243495
β-strand2443-244424
β-strand2447-245043
β-strand245311
α-helix2467-248216
α-helix2489-24924
α-helix2501-251616
α-helix2519-253416
α-helix2536-25427
α-helix2545-25462

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Piezo-type mechanosensitive ion channel component 1A, C, Eprotein2547Mus musculusE2JF22 (AlphaFold model)
Sequence of entity 1 (A, C, E), FASTA
>7WLT_1 Piezo-type mechanosensitive ion channel component 1 (chains A, C, E)
MEPHVLGAGLYWLLLPCTLLAASLLRFNALSLVYLLFLLLLPWLPGPSRHSIPGHTGRLL
RALLCLSLLFLVAHLAFQICLHTVPHLDQFLGQNGSLWVKVSQHIGVTRLDLKDIFNTTR
LVAPDLGVLLASSLCLGLCGRLTRKAGQSRRTQELQDDDDDDDDDDEDIDAAPAVGLKGA
PALATKRRLWLASRFRVTAHWLLMTSGRTLVIVLLALAGIAHPSAFSSIYLVVFLAICTW
WSCHFPLSPLGFNTLCVMVSCFGAGHLICLYCYQTPFIQDMLPPGNIWARLFGLKNFVDL
PNYSSPNALVLNTKHAWPIYVSPGILLLLYYTATSLLKLHKSCPSELRKETPREDEEHEL
ELDHLEPEPQARDATQGEMPMTTEPDLDNCTVHVLTSQSPVRQRPVRPRLAELKEMSPLH
GLGHLIMDQSYVCALIAMMVWSIMYHSWLTFVLLLWACLIWTVRSRHQLAMLCSPCILLY
GLTLCCLRYVWAMELPELPTTLGPVSLHQLGLEHTRYPCLDLGAMLLYLLTFWLLLRQFV
KEKLLKKQKVPAALLEVTVADTEPTQTQTLLRSLGELVTGIYVKYWIYVCAGMFIVVSFA
GRLVVYKIVYMFLFLLCLTLFQVYYTLWRKLLRVFWWLVVAYTMLVLIAVYTFQFQDFPT
YWRNLTGFTDEQLGDLGLEQFSVSELFSSILIPGFFLLACILQLHYFHRPFMQLTDLEHV
PPPGTRHPRWAHRQDAVSEAPLLEHQEEEEVFREDGQSMDGPHQATQVPEGTASKWGLVA
DRLLDLAASFSAVLTRIQVFVRRLLELHVFKLVALYTVWVALKEVSVMNLLLVVLWAFAL
PYPRFRPMASCLSTVWTCIIIVCKMLYQLKIVNPHEYSSNCTEPFPNNTNLQPLEINQSL
LYRGPVDPANWFGVRKGYPNLGYIQNHLQILLLLVFEAVVYRRQEHYRRQHQQAPLPAQA
VCADGTRQRLDQDLLSCLKYFINFFFYKFGLEICFLMAVNVIGQRMNFMVILHGCWLVAI
LTRRRREAIARLWPNYCLFLTLFLLYQYLLCLGMPPALCIDYPWRWSKAIPMNSALIKWL
YLPDFFRAPNSTNLISDFLLLLCASQQWQVFSAERTEEWQRMAGINTDHLEPLRGEPNPI
PNFIHCRSYLDMLKVAVFRYLFWLVLVVVFVAGATRISIFGLGYLLACFYLLLFGTTLLQ
KDTRAQLVLWDCLILYNVTVIISKNMLSLLSCVFVEQMQSNFCWVIQLFSLVCTVKGYYD
PKEMMTRDRDCLLPVEEAGIIWDSICFFFLLLQRRIFLSHYFLHVSADLKATALQASRGF
ALYNAANLKSINFHRQIEEKSLAQLKRQMKRIRAKQEKYRQSQASRGQLQSKDPQDPSQE
PGPDSPGGSSPPRRQWWRPWLDHATVIHSGDYFLFESDSEEEEEALPEDPRPAAQSAFQM
AYQAWVTNAQTVLRQRRERARQERAEQLASGGDLNPDVEPVDVPEDEMAGRSHMMQRVLS
TMQFLWVLGQATVDGLTRWLRAFTKHHRTMSDVLCAERYLLTQELLRVGEVRRGVLDQLY
VGEDEATLSGPVETRDGPSTASSGLGAEEPLSSMTDDTSSPLSTGYNTRSGSEEIVTDAG
DLQAGTSLHGSQELLANARTRMRTASELLLDRRLHIPELEEAERFEAQQGRTLRLLRAGY
QCVAAHSELLCYFIIILNHMVTASAASLVLPVLVFLWAMLTIPRPSKRFWMTAIVFTEVM
VVTKYLFQFGFFPWNSYVVLRRYENKPYFPPRILGLEKTDSYIKYDLVQLMALFFHRSQL
LCYGLWDHEEDRYPKDHCRSSVKDREAKEEPEAKLESQSETGTGHPKEPVLAGTPRDHIQ
GKGSIRSKDVIQDPPEDLKPRHTRHISIRFRRRKETPGPKGTAVMETEHEEGEGKETTER
KRPRHTQEKSKFRERMKAAGRRLQSFCVSLAQSFYQPLQRFFHDILHTKYRAATDVYALM
FLADIVDIIIIIFGFWAFGKHSAATDIASSLSDDQVPQAFLFMLLVQFGTMVIDRALYLR
KTVLGKLAFQVVLVVAIHIWMFFILPAVTERMFSQNAVAQLWYFVKCIYFALSAYQIRCG
YPTRILGNFLTKKYNHLNLFLFQGFRLVPFLVELRAVMDWVWTDTTLSLSNWMCVEDIYA
NIFIIKCSRETEKKYPQPKGQKKKKIVKYGMGGLIILFLIAIIWFPLLFMSLIRSVVGVV
NQPIDVTVTLKLGGYEPLFTMSAQQPSIVPFTPQAYEELSQQFDPYPLAMQFISQYSPED
IVTAQIEGSSGALWRISPPSRAQMKQELYNGTADITLRFTWNFQRDLAKGGTVEYTNEKH
TLELAPNSTARRQLAQLLEGRPDQSVVIPHLFPKYIRAPNGPEANPVKQLQPDEEEDYLG
VRIQLRREQVGTGASGEQAGTKASDFLEWWVIELQDCKADCNLLPMVIFSDKVSPPSLGF
LAGYGIVGLYVSIVLVVGKFVRGFFSEISHSIMFEELPCVDRILKLCQDIFLVRETRELE
LEEELYAKLIFLYRSPETMIKWTRERE

Ligands and cofactors

IDNameFormulaCopies
P5SO-[(R)-{[(2R)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-L-serineC42 H82 N O10 P3
PEE1,2-dioleoyl-sn-glycero-3-phosphoethanolamineC41 H78 N O8 P3
PLX(9R,11S)-9-({[(1S)-1-hydroxyhexadecyl]oxy}methyl)-2,2-dimethyl-5,7,10-trioxa-2L…C42 H89 N O8 P21

Primary citation

Structure deformation and curvature sensing of PIEZO1 in lipid membranes. Yang, X., Lin, C., Chen, X. et al. Nature (2022) 604:377-383. DOI 10.1038/s41586-022-04574-8 · PubMed

Other PDB entries of the same protein (UniProt E2JF22 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

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