8G24: MAP domain-containing protein

Crystal Structure of Cathepsin-G and Neutrophil Elastase Inhibited by S. aureus EapH2 at pH 5.5. Determined by X-ray diffraction at 1.82 Å resolution. Released 26 Apr 2023.

Method: X-ray diffraction
Resolution: 1.82 Å
Organisms: Staphylococcus aureus subsp. aureus Mu50, Homo sapiens
Chains: 3
Atoms: 4,426
Mol. weight: 62.26 kDa
Released: 26 Apr 2023

Explore 8G24 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8G24 contains 16 α-helices and 53 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 8 helices, 21 β-strands

Element	Residues	Length	Sheet
β-strand	17	1	10
β-strand	20-21	2	2
α-helix	22-23	2
β-strand	30-37	8	1
β-strand	40-50	11	1
β-strand	53-56	4	1
α-helix	58-60	3
β-strand	64-69	6	1
β-strand	73	1	11
β-strand	82-91	10	1
β-strand	96	1	12
β-strand	101	1	12
β-strand	105-109	5	1
β-strand	116	1	13
β-strand	119	1	13
α-helix	123-126	4
β-strand	136-141	6	2
β-strand	153	1	11
β-strand	155-161	7	2
α-helix	165-168	4
β-strand	179-182	4	2
β-strand	190	1	10
β-strand	199-202	4	2
β-strand	205-213	9	2
α-helix	218	1
α-helix	220	1
β-strand	221-225	5	2
α-helix	226-229	4
α-helix	230-237	8

Chain B: 5 helices, 22 β-strands

Element	Residues	Length	Sheet
β-strand	30	1	5
β-strand	33-34	2	4
α-helix	35-36	2
β-strand	43-48	6	6
β-strand	51-60	10	6
β-strand	63-66	4	6
α-helix	68-70	3
α-helix	76-78	3
β-strand	80-83	4	6
β-strand	87	1	7
β-strand	96-105	10	6
β-strand	109	1	8
β-strand	114	1	8
β-strand	118-122	5	6
β-strand	149-154	6	4
β-strand	157	1	9
β-strand	164	1	9
β-strand	167	1	7
β-strand	169-176	8	4
β-strand	185-188	4	4
β-strand	195	1	5
β-strand	204-207	4	4
β-strand	210-219	10	4
β-strand	221	1	4
α-helix	228	1
β-strand	229-233	5	4
α-helix	234-245	12

Chain C: 3 helices, 10 β-strands

Element	Residues	Length	Sheet
β-strand	45-54	10	1
β-strand	57	1	1
β-strand	58-59	2	2
β-strand	62-68	7	1
β-strand	72-73	2	3
α-helix	75-90	16
α-helix	94-99	6
β-strand	102-109	8	1
β-strand	114-118	5	1
β-strand	123-125	3	4
β-strand	129-130	2	3
α-helix	131-133	3
β-strand	134-142	9	1

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
MAP domain-containing protein	C	protein	117	Staphylococcus aureus subsp. aureus Mu50	A0A0H3JUK5 (AlphaFold model)
Neutrophil elastase	B	protein	218	Homo sapiens	P08246 (AlphaFold model)
Cathepsin-G	A	protein	223	Homo sapiens	P08311 (AlphaFold model)

Sequence of entity 1 (C), FASTA

>8G24_1 MAP domain-containing protein (chains C)
GSTAEKDKLPATQKAKEMQNVPYTIAVDGIMAFNQSYLNLPKDSQLSYLDLGNKVKALLY
DERGVTPEKIRNAKSAVYTITWKDGSKKEVDLKKDSYTANLFDSNSIKQIDINVKTK

Sequence of entity 2 (B), FASTA

>8G24_2 Neutrophil elastase (chains B)
IVGGRRARPHAWPFMVSLQLRGGHFCGATLIAPNFVMSAAHCVANVNVRAVRVVLGAHNL
SRREPTRQVFAVQRIFENGYDPVNLLNDIVILQLNGSATINANVQVAQLPAQGRRLGNGV
QCLAMGWGLLGRNRGIASVLQELNVTVVTSLCRRSNVCTLVRGRQAGVCFGDSGSPLVCN
GLIHGIASFVRGGCASGLYPDAFAPVAQFVNWIDSIIQ

Sequence of entity 3 (A), FASTA

>8G24_3 Cathepsin-G (chains A)
IIGGRESRPHSRPYMAYLQIQSPAGQSRCGGFLVREDFVLTAAHCWGSNINVTLGAHNIQ
RRENTQQHITARRAIRHPQYNQRTIQNDIMLLQLSRRVRRNRNVNPVALPRAQEGLRPGT
LCTVAGWGRVSMRRGTDTLREVQLRVQRDRQCLRIFGSYDPRRQICVGDRRERKAAFKGD
SGGPLLCNNVAHGIVSYGKSSGVPPEVFTRVSSFLPWIRTTMR

Primary citation

Simultaneous inhibition of two neutrophil serine proteases by the S. aureus innate immune evasion protein EapH2. Mishra, N., Herdendorf, T.J., Prakash, O. et al. J Biol Chem (2023) 299:104878-104878. DOI 10.1016/j.jbc.2023.104878 · PubMed

Other PDB entries of the same protein (UniProt A0A0H3JUK5 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8G25 1.8 Å, Crystal Structure of Cathepsin-G and Neutrophil Elastase Inhibited by S. aureus EapH2 at…
8G26 1.85 Å, Crystal Structure of Cathepsin-G and Neutrophil Elastase Inhibited by S. aureus EapH2 at…
1YN5 2.2 Å, Crystal Structures of EAP Domains from Staphylococcus aureus Reveal an Unexpected…
8GDG Solution structure of the Neutrophil Serine Protease Inhibitor, EapH2

Browse structure collections

About this viewer

MolViewer shows 8G24 directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.