8IXN: Curved structure of mPIEZO1-S2472E

Curved structure of mPIEZO1-S2472E. Determined by electron microscopy at 4.07 Å resolution. Released 1 Jan 2025.

Method
Electron microscopy
Resolution
4.07 Å
Organism
Mus musculus
Chains
3
Atoms
30,043
Mol. weight
886.3 kDa
Ligands
P5S, PEE, PLX
Released
1 Jan 2025

Explore 8IXN in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8IXN contains 194 α-helices and 56 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 66 helices, 20 β-strands

ElementResiduesLengthSheet
α-helix577-5848
α-helix587-59711
α-helix605-62420
α-helix632-64716
α-helix688-6903
α-helix691-70212
α-helix703-7075
α-helix708-7158
α-helix787-80519
α-helix809-82315
α-helix830-83910
α-helix843-8453
α-helix846-86015
α-helix863-8675
α-helix922-94928
α-helix969-9713
α-helix974-100532
α-helix1008-102114
α-helix1026-105126
α-helix1074-10796
α-helix1094-110916
α-helix1142-11443
α-helix1149-115911
α-helix1161-117515
α-helix1182-120019
α-helix1203-123129
α-helix1234-12385
α-helix1243-12497
α-helix1281-129818
α-helix1300-131415
α-helix1316-136449
α-helix1412-14154
α-helix1506-154439
α-helix1553-15564
α-helix1657-166812
α-helix1674-168613
α-helix1689-170012
α-helix1709-17135
α-helix1714-17185
α-helix1726-174823
α-helix1783-180018
α-helix1957-19659
α-helix1978-199316
α-helix2016-203924
α-helix2044-205916
α-helix2060-20634
α-helix2078-209922
α-helix2116-212611
α-helix2132-214211
α-helix2149-217426
α-helix2182-21843
α-helix2185-221228
β-strand222017
β-strand2225-223288
β-strand2236-224168
β-strand2248-225039
α-helix2251-22522
α-helix2253-226210
α-helix2267-22759
α-helix2278-22803
β-strand2281-228669
α-helix2298-230912
β-strand2316110
β-strand231718
β-strand2319-232468
β-strand2335-234068
β-strand2343110
α-helix2349-235911
β-strand2372111
β-strand2375-237849
β-strand238619
α-helix2394-23963
β-strand2399111
β-strand2401112
β-strand2404-2405213
α-helix2421-24255
β-strand2430-2431213
β-strand2434112
β-strand2444-245079
β-strand245217
α-helix2456-24583
α-helix2466-248621
α-helix2492-24943
α-helix2501-251515
α-helix2522-253312
α-helix2536-25427
Chain C: 63 helices, 18 β-strands
ElementResiduesLengthSheet
α-helix577-5848
α-helix587-59711
α-helix605-62420
α-helix632-64716
α-helix691-70212
α-helix703-7075
α-helix708-7158
α-helix787-82337
α-helix830-83910
α-helix843-8453
α-helix846-86015
α-helix863-8675
α-helix922-94928
α-helix974-100532
α-helix1008-102114
α-helix1026-105126
α-helix1074-10796
α-helix1094-110916
α-helix1142-11443
α-helix1149-115911
α-helix1161-117515
α-helix1182-120019
α-helix1203-123129
α-helix1234-12385
α-helix1243-12497
α-helix1281-129818
α-helix1300-131415
α-helix1316-136449
α-helix1412-14154
α-helix1506-154439
α-helix1553-15564
α-helix1657-166812
α-helix1674-168613
α-helix1689-170012
α-helix1709-17135
α-helix1714-17185
α-helix1726-174722
α-helix1783-180018
α-helix1957-19659
α-helix1978-199316
α-helix2016-203924
α-helix2044-205916
α-helix2060-20634
α-helix2078-209922
α-helix2116-212611
α-helix2132-214211
α-helix2149-217426
α-helix2182-21843
α-helix2185-221228
β-strand222011
β-strand2225-223282
β-strand2236-224162
β-strand2248-225033
α-helix2251-22522
α-helix2253-226210
α-helix2267-22759
α-helix2278-22803
β-strand2281-228663
α-helix2298-230912
β-strand231614
β-strand231712
β-strand2319-232462
β-strand2335-234062
β-strand234314
α-helix2349-235911
β-strand237215
β-strand2376-237833
β-strand238613
α-helix2394-23963
β-strand239915
β-strand2401-240556
α-helix2421-24255
β-strand2430-243456
β-strand2444-245073
β-strand245211
α-helix2456-24583
α-helix2466-248621
α-helix2492-24943
α-helix2501-251515
α-helix2522-253211
α-helix2536-25427
Chain D: 65 helices, 18 β-strands
ElementResiduesLengthSheet
α-helix577-5848
α-helix587-59711
α-helix605-62420
α-helix632-64716
α-helix691-70212
α-helix703-7075
α-helix708-7158
α-helix787-80519
α-helix809-82315
α-helix830-83910
α-helix843-8453
α-helix846-86015
α-helix863-8675
α-helix922-94928
α-helix969-9713
α-helix974-100532
α-helix1008-102114
α-helix1026-105126
α-helix1074-10796
α-helix1094-110916
α-helix1142-11443
α-helix1149-115911
α-helix1161-117515
α-helix1182-119918
α-helix1203-123129
α-helix1234-12385
α-helix1243-12497
α-helix1281-129818
α-helix1300-131415
α-helix1316-136449
α-helix1412-14154
α-helix1506-154439
α-helix1553-15564
α-helix1657-166812
α-helix1674-168613
α-helix1689-170012
α-helix1709-17135
α-helix1714-17185
α-helix1726-174722
α-helix1783-180018
α-helix1957-19659
α-helix1978-199316
α-helix2016-203924
α-helix2044-205916
α-helix2060-20634
α-helix2078-209922
α-helix2116-212611
α-helix2132-214211
α-helix2149-217426
α-helix2182-21843
α-helix2185-221228
β-strand2220114
β-strand2225-2232815
β-strand2236-2241615
β-strand2248-2250316
α-helix2251-22522
α-helix2253-226210
α-helix2267-22759
α-helix2278-22803
β-strand2281-2286616
α-helix2298-230912
β-strand2316117
β-strand2317115
β-strand2319-2324615
β-strand2335-2340615
β-strand2343117
α-helix2349-235911
β-strand2372118
β-strand2375-2378416
β-strand2386116
α-helix2394-23963
β-strand2399118
β-strand2401-2405519
α-helix2421-24255
β-strand2430-2434519
β-strand2444-2450716
β-strand2452114
α-helix2456-24583
α-helix2466-248621
α-helix2492-24943
α-helix2501-251515
α-helix2522-253312
α-helix2536-25416

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Piezo-type mechanosensitive ion channel component 1A, C, Dprotein2547Mus musculusE2JF22 (AlphaFold model)
Sequence of entity 1 (A, C, D), FASTA
>8IXN_1 Piezo-type mechanosensitive ion channel component 1 (chains A, C, D)
MEPHVLGAGLYWLLLPCTLLAASLLRFNALSLVYLLFLLLLPWLPGPSRHSIPGHTGRLL
RALLCLSLLFLVAHLAFQICLHTVPHLDQFLGQNGSLWVKVSQHIGVTRLDLKDIFNTTR
LVAPDLGVLLASSLCLGLCGRLTRKAGQSRRTQELQDDDDDDDDDDEDIDAAPAVGLKGA
PALATKRRLWLASRFRVTAHWLLMTSGRTLVIVLLALAGIAHPSAFSSIYLVVFLAICTW
WSCHFPLSPLGFNTLCVMVSCFGAGHLICLYCYQTPFIQDMLPPGNIWARLFGLKNFVDL
PNYSSPNALVLNTKHAWPIYVSPGILLLLYYTATSLLKLHKSCPSELRKETPREDEEHEL
ELDHLEPEPQARDATQGEMPMTTEPDLDNCTVHVLTSQSPVRQRPVRPRLAELKEMSPLH
GLGHLIMDQSYVCALIAMMVWSIMYHSWLTFVLLLWACLIWTVRSRHQLAMLCSPCILLY
GLTLCCLRYVWAMELPELPTTLGPVSLHQLGLEHTRYPCLDLGAMLLYLLTFWLLLRQFV
KEKLLKKQKVPAALLEVTVADTEPTQTQTLLRSLGELVTGIYVKYWIYVCAGMFIVVSFA
GRLVVYKIVYMFLFLLCLTLFQVYYTLWRKLLRVFWWLVVAYTMLVLIAVYTFQFQDFPT
YWRNLTGFTDEQLGDLGLEQFSVSELFSSILIPGFFLLACILQLHYFHRPFMQLTDLEHV
PPPGTRHPRWAHRQDAVSEAPLLEHQEEEEVFREDGQSMDGPHQATQVPEGTASKWGLVA
DRLLDLAASFSAVLTRIQVFVRRLLELHVFKLVALYTVWVALKEVSVMNLLLVVLWAFAL
PYPRFRPMASCLSTVWTCIIIVCKMLYQLKIVNPHEYSSNCTEPFPNNTNLQPLEINQSL
LYRGPVDPANWFGVRKGYPNLGYIQNHLQILLLLVFEAVVYRRQEHYRRQHQQAPLPAQA
VCADGTRQRLDQDLLSCLKYFINFFFYKFGLEICFLMAVNVIGQRMNFMVILHGCWLVAI
LTRRRREAIARLWPNYCLFLTLFLLYQYLLCLGMPPALCIDYPWRWSKAIPMNSALIKWL
YLPDFFRAPNSTNLISDFLLLLCASQQWQVFSAERTEEWQRMAGINTDHLEPLRGEPNPI
PNFIHCRSYLDMLKVAVFRYLFWLVLVVVFVAGATRISIFGLGYLLACFYLLLFGTTLLQ
KDTRAQLVLWDCLILYNVTVIISKNMLSLLSCVFVEQMQSNFCWVIQLFSLVCTVKGYYD
PKEMMTRDRDCLLPVEEAGIIWDSICFFFLLLQRRIFLSHYFLHVSADLKATALQASRGF
ALYNAANLKSINFHRQIEEKSLAQLKRQMKRIRAKQEKYRQSQASRGQLQSKDPQDPSQE
PGPDSPGGSSPPRRQWWRPWLDHATVIHSGDYFLFESDSEEEEEALPEDPRPAAQSAFQM
AYQAWVTNAQTVLRQRRERARQERAEQLASGGDLNPDVEPVDVPEDEMAGRSHMMQRVLS
TMQFLWVLGQATVDGLTRWLRAFTKHHRTMSDVLCAERYLLTQELLRVGEVRRGVLDQLY
VGEDEATLSGPVETRDGPSTASSGLGAEEPLSSMTDDTSSPLSTGYNTRSGSEEIVTDAG
DLQAGTSLHGSQELLANARTRMRTASELLLDRRLHIPELEEAERFEAQQGRTLRLLRAGY
QCVAAHSELLCYFIIILNHMVTASAASLVLPVLVFLWAMLTIPRPSKRFWMTAIVFTEVM
VVTKYLFQFGFFPWNSYVVLRRYENKPYFPPRILGLEKTDSYIKYDLVQLMALFFHRSQL
LCYGLWDHEEDRYPKDHCRSSVKDREAKEEPEAKLESQSETGTGHPKEPVLAGTPRDHIQ
GKGSIRSKDVIQDPPEDLKPRHTRHISIRFRRRKETPGPKGTAVMETEHEEGEGKETTER
KRPRHTQEKSKFRERMKAAGRRLQSFCVSLAQSFYQPLQRFFHDILHTKYRAATDVYALM
FLADIVDIIIIIFGFWAFGKHSAATDIASSLSDDQVPQAFLFMLLVQFGTMVIDRALYLR
KTVLGKLAFQVVLVVAIHIWMFFILPAVTERMFSQNAVAQLWYFVKCIYFALSAYQIRCG
YPTRILGNFLTKKYNHLNLFLFQGFRLVPFLVELRAVMDWVWTDTTLSLSNWMCVEDIYA
NIFIIKCSRETEKKYPQPKGQKKKKIVKYGMGGLIILFLIAIIWFPLLFMSLIRSVVGVV
NQPIDVTVTLKLGGYEPLFTMSAQQPSIVPFTPQAYEELSQQFDPYPLAMQFISQYSPED
IVTAQIEGSSGALWRISPPSRAQMKQELYNGTADITLRFTWNFQRDLAKGGTVEYTNEKH
TLELAPNSTARRQLAQLLEGRPDQSVVIPHLFPKYIRAPNGPEANPVKQLQPDEEEDYLG
VRIQLRREQVGTGASGEQAGTKASDFLEWWVIELQDCKADCNLLPMVIFSDKVSPPSLGF
LAGYGIVGLYVEIVLVVGKFVRGFFSEISHSIMFEELPCVDRILKLCQDIFLVRETRELE
LEEELYAKLIFLYRSPETMIKWTRERE

Ligands and cofactors

IDNameFormulaCopies
P5SO-[(R)-{[(2R)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-L-serineC42 H82 N O10 P3
PEE1,2-dioleoyl-sn-glycero-3-phosphoethanolamineC41 H78 N O8 P3
PLX(9R,11S)-9-({[(1S)-1-hydroxyhexadecyl]oxy}methyl)-2,2-dimethyl-5,7,10-trioxa-2L…C42 H89 N O8 P6

Primary citation

An intermediate open structure reveals the gating transition of the mechanically activated PIEZO1 channel. Liu, S., Yang, X., Chen, X. et al. Neuron (2025) 113:590. DOI 10.1016/j.neuron.2024.11.020 · PubMed

Other PDB entries of the same protein (UniProt E2JF22 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

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