9B5Y: LAPTH-bound PTH1R

Cryo-EM structure of the LAPTH-bound PTH1R in complex with Gq. Determined by electron microscopy at 3.49 Å resolution. Released 19 Mar 2025.

Method: Electron microscopy
Resolution: 3.49 Å
Organisms: Homo sapiens, Mus musculus
Chains: 6
Atoms: 8,429
Mol. weight: 199.03 kDa
Released: 19 Mar 2025

Explore 9B5Y in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9B5Y contains 34 α-helices and 63 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 9 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	13-34	22
β-strand	40-45	6	1
α-helix	54-59	6
β-strand	191	1	1
β-strand	196	1	2
β-strand	201	1	2
β-strand	203-206	4	1
α-helix	218-221	4
β-strand	225-231	7	1
α-helix	232-234	3
α-helix	248-259	12
β-strand	269-274	6	1
α-helix	276-285	10
α-helix	299-301	3
α-helix	304-315	12
β-strand	326	1	1
α-helix	338-355	18

Chain B: 2 helices, 29 β-strands

Element	Residues	Length	Sheet
α-helix	3-24	22
α-helix	30-34	5
β-strand	49-50	2	3
β-strand	58-62	5	4
β-strand	69-74	6	4
β-strand	78-83	6	4
β-strand	88-94	7	4
β-strand	100-105	6	5
β-strand	111-116	6	5
β-strand	120-125	6	5
β-strand	134-140	7	5
β-strand	146-151	6	6
β-strand	156-161	6	6
β-strand	165-170	6	6
β-strand	176-181	6	6
β-strand	187-192	6	7
β-strand	198-203	6	7
β-strand	207-212	6	7
β-strand	218-223	6	7
β-strand	229-232	4	8
β-strand	240-245	6	8
β-strand	250-254	5	8
β-strand	260-264	5	8
β-strand	273-278	6	9
β-strand	284-289	6	9
β-strand	293	1	9
β-strand	296-298	3	9
β-strand	304-306	3	9
β-strand	315-320	6	3
β-strand	327-331	5	3
β-strand	335-339	5	3

Chain G: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	7-23	17
α-helix	30-42	13
α-helix	45-47	3
α-helix	53-55	3

Chain L: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	3-20	18

Chain N: 5 helices, 26 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	10
β-strand	11-12	2	11
β-strand	18-25	8	10
β-strand	33-39	7	12
β-strand	45-51	7	12
α-helix	53-55	3
β-strand	58-60	3	12
α-helix	62-64	3
β-strand	68-73	6	10
β-strand	78-83	6	10
α-helix	88-90	3
β-strand	92-99	8	12
β-strand	110-111	2	12
β-strand	115-117	3	12
β-strand	118-119	2	11
β-strand	142	1	13
β-strand	147-149	3	14
β-strand	156-157	2	15
β-strand	160-161	2	13
β-strand	167	1	16
β-strand	173	1	16
β-strand	176-180	5	14
β-strand	187-191	5	14
β-strand	195-196	2	14
α-helix	197	1
β-strand	206-208	3	13
β-strand	212-215	4	13
β-strand	216-217	2	15
α-helix	222-224	3
β-strand	227-231	5	14
β-strand	244-247	4	14

Chain R: 13 helices, 0 β-strands

Element	Residues	Length
α-helix	180-211	32
α-helix	218-243	26
α-helix	280-309	30
α-helix	318-321	4
α-helix	323-325	3
α-helix	326-330	5
α-helix	331-338	8
α-helix	365-390	26
α-helix	403-417	15
α-helix	435-444	10
α-helix	456	1
α-helix	457-461	5
α-helix	466-469	4

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Parathyroid hormone/parathyroid hormone-related peptide receptor	R	protein	693	Homo sapiens	Q03431 (AlphaFold model)
Guanine nucleotide-binding protein G(q)	A	protein	353	Homo sapiens	A0A8D2GGC9 (AlphaFold model)
Long-acting parathyroid hormone analog	L	protein	36	Homo sapiens	F5GZD9 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	351	Homo sapiens	P62873 (AlphaFold model)
scFv16	N	protein	266	Mus musculus
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768

Sequence of entity 1 (R), FASTA

>9B5Y_1 Parathyroid hormone/parathyroid hormone-related peptide receptor (chains R)
DYKDDDDGQPGNGSAFLLAPNGSHAPDHNVTQQRDEENLYFQGVDGGSGGSGGDADDVMT
KEEQIFLLHRAQAQCEKRLKEVLQRPASIMESDKGWTSASTSGKPRKDKASGKLYPESEE
DKEAPTGSRYRGRPCLPEWDHILCWPLGAPGEVVAVPCPDYIYDFNHKGHAYRRCDRNGS
WELVPGHNRTWANYSECVKFLTNETREREVFDRLGMIYTVGYSVSLASLTVAVLILAYFR
RLHCTRNYIHMHLFLSFMLRAVSIFVKDAVLYSGATLDEAERLTEEELRAIAQAPPPPAT
AAAGYAGCRVAVTFFLYFLATNYYWILVEGLYLHSLIFMAFFSEKKYLWGFTVFGWGLPA
VFVAVWVSVRATLANTGCWDLSSGNKKWIIQVPILASIVLNFILFINIVRVLATKLRETN
AGRCDTRQQYRKLLKSTLVLMPLFGVHYIVFMATPYTEVSGTLWQVQMHYEMLFNSFQGF
FVAIIYCFCNGEVQAEIKKSWSRWTLALDFKRKARSGSSSYSYGAAAVFTLEDFVGDWEQ
TAAYNLDQVLEQGGVSSLLQNLAVSVTPIQRIVRSGENALKIDIHVIIPYEGLSADQMAQ
IEEVFKVVYPVDDHHFKVILPYGTLVIDGVTPNMLNYFGRPYEGIAVFDGKKITVTGTLW
NGNKIIDERLITPDGSMLFRVTINSHHHHHHHH

Sequence of entity 2 (A), FASTA

>9B5Y_2 Guanine nucleotide-binding protein G(q) (chains A)
MGCTLSAEDKAAVERSKMIDRNLREDGEKARRELKLLLLGTGESGKSTFIKQMRIIHGSG
YSDEDKRGFTKLVYQNIFTAMQAMIRAMDTLKIPYKYEHNKAHAQLVREVDVEKVSAFEN
PYVDAIKSLWNDPGIQECYDRRREYQLSDSTKYYLNDLDRVADPAYLPTQQDVLRVRVPT
TGIIEYPFDLQSVIFRMVDVGGQRSERRKWIHCFENVTSIMFLVALSEYDQVLVESDNEN
RMEESKALFRTIITYPWFQNSSVILFLNKKDLLEEKIMYSHLVDYFPEYDGPQRDAQAAR
EFILKMFVDLNPDSDKIIYSHFTCATDTENIRFVFAAVKDTILQLNLKEYNLV

Sequence of entity 3 (L), FASTA

>9B5Y_3 Long-acting parathyroid hormone analog (chains L)
AVAEIQLMHQRAKWIQDARRRAFLHKLIAEIHTAEI

Sequence of entity 4 (B), FASTA

>9B5Y_4 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRR
TLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSG
NYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALW
DIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDI
NAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGY
DDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 5 (N), FASTA

>9B5Y_5 scFv16 (chains N)
VQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYYA
DTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVSA
GGGGSGGGGSGGGGSADIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLELLEENLYFQGASHHHHHHHH

Sequence of entity 6 (G), FASTA

>9B5Y_6 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Primary citation

Allosteric mechanism in the distinctive coupling of G q and G s to the parathyroid hormone type 1 receptor. Zhang, X., Lee, J.Y., Pacheco, J. et al. Proc Natl Acad Sci U S A (2025) 122:e2426178122-e2426178122. DOI 10.1073/pnas.2426178122 · PubMed

Other PDB entries of the same protein (UniProt Q03431 (AlphaFold model), which also has an AlphaFold model), best resolution first:

5EMB 0.85 Å, Crystal structure of the SNX27 PDZ domain bound to the C-terminal phosphorylated PTHR…
4Z8J 0.95 Å, Crystal structure of the SNX27 PDZ domain bound to the C-terminal PTHR PDZ binding motif
7UZO 1.3 Å, Parathyroid hormone 1 receptor extracellular domain complexed with a peptide ligand…
3H3G 1.94 Å, Crystal structure of the extracellular domain of the human parathyroid hormone receptor…
3C4M 1.95 Å, Structure of human parathyroid hormone in complex with the extracellular domain of its…
8D51 2.0 Å, Parathyroid hormone 1 receptor extracellular domain complexed with a peptide ligand…
8D52 2.02 Å, Parathyroid hormone 1 receptor extracellular domain complexed with a peptide ligand…
7UZP 2.29 Å, parathyroid hormone 1 receptor extracellular domain complexed with a peptide ligand…
8BIA 2.4 Å, Crystal structure of Scribble PDZ1 with PTHR
6FJ3 2.5 Å, High resolution crystal structure of parathyroid hormone 1 receptor in complex with a…
8FLQ 2.55 Å, Human PTH1R in complex with PTH(1-34) and Gs
8JR9 2.57 Å, Small molecule agonist (PCO371) bound to human parathyroid hormone receptor type 1 (PTH1R)

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