9E2X: DNA replication complex GINS protein PSF1

Cryo-EM structure of yeast CMG helicase stalled at G4-containing DNA template, state 2. Determined by electron microscopy at 3.5 Å resolution. Released 19 Mar 2025.

Method: Electron microscopy
Resolution: 3.5 Å
Organisms: Saccharomyces cerevisiae W303, synthetic construct
Chains: 15
Atoms: 49,316
Mol. weight: 965.18 kDa
Ligands: ATP, MG, ZN, ADP
Released: 19 Mar 2025

Explore 9E2X in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9E2X contains 296 α-helices and 235 β-strands across 13 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain 2: 28 helices, 30 β-strands

Element	Residues	Length	Sheet
α-helix	176-178	3
α-helix	183-187	5
α-helix	194-199	6
α-helix	201-217	17
β-strand	219	1	14
β-strand	225	1	14
α-helix	226-236	11
β-strand	241-244	4	15
α-helix	246-252	7
α-helix	254-262	9
α-helix	264-282	19
α-helix	286-289	4
β-strand	294-297	4	15
β-strand	303-304	2	16
α-helix	311-313	3
β-strand	317-327	11	16
β-strand	331-334	4	16
β-strand	336-341	6	17
β-strand	347-352	6	17
β-strand	373-375	3	17
β-strand	382-391	10	16
β-strand	405-411	7	16
β-strand	423-434	12	16
α-helix	436-442	7
β-strand	446	1	18
β-strand	447-458	12	16
α-helix	478-488	11
α-helix	493-499	7
α-helix	509-520	12
β-strand	525-527	3	19
β-strand	531-533	3	19
β-strand	539-542	4	20
α-helix	550-559	10
β-strand	563-567	5	20
β-strand	571	1	21
α-helix	573-577	5
β-strand	580-582	3	22
β-strand	589-591	3	22
α-helix	595-598	4
β-strand	603-607	5	20
α-helix	609-611	3
α-helix	614-625	12
β-strand	628-631	4	23
β-strand	638-641	4	23
β-strand	645-650	6	20
α-helix	663-666	4
α-helix	671-676	6
β-strand	679-682	4	20
β-strand	686	1	24
α-helix	689-705	17
α-helix	740-755	16
α-helix	760-773	14
β-strand	777	1	25
α-helix	783-797	15
α-helix	807-823	17
β-strand	828	1	25
α-helix	831-848	18
α-helix	851-860	10

Chain 3: 24 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	20-34	15
α-helix	36-51	16
β-strand	95-99	5	26
α-helix	100-106	7
α-helix	108-116	9
α-helix	118-136	19
α-helix	144-148	5
β-strand	154-158	5	26
β-strand	165-166	2	27
α-helix	173-175	3
β-strand	179-189	11	27
α-helix	190-192	3
β-strand	193-203	11	27
β-strand	208-213	6	27
β-strand	234	1	28
β-strand	240	1	28
β-strand	242-259	18	27
α-helix	266	1
β-strand	273-279	7	27
β-strand	291-301	11	27
β-strand	318-328	11	27
α-helix	344-355	12
α-helix	359-366	8
α-helix	375-386	12
β-strand	391-392	2	29
β-strand	398-399	2	29
β-strand	405-409	5	30
α-helix	415-425	11
β-strand	433	1	31
β-strand	445-447	3	32
β-strand	456-458	3	32
α-helix	463-465	3
β-strand	468-471	4	30
β-strand	473	1	31
α-helix	475-477	3
α-helix	480-492	13
β-strand	494-499	6	33
β-strand	502-507	6	33
β-strand	510-516	7	30
α-helix	529-532	4
α-helix	537-542	6
β-strand	545-549	5	30
α-helix	555-570	16
β-strand	622	1	34
α-helix	632-638	7
β-strand	651	1	34
α-helix	653-666	14
β-strand	670	1	35
α-helix	673-688	16
α-helix	700-715	16
β-strand	720	1	35
α-helix	723-737	15

Chain 4: 29 helices, 35 β-strands

Element	Residues	Length	Sheet
β-strand	177-180	4	36
β-strand	186	1	36
α-helix	187-199	13
β-strand	202	1	37
α-helix	204-209	6
α-helix	213-215	3
β-strand	224	1	37
α-helix	225-236	12
β-strand	240-244	5	38
α-helix	245-250	6
α-helix	255-263	9
α-helix	265-287	23
α-helix	293-297	5
β-strand	302-306	5	38
α-helix	319-321	3
β-strand	325-335	11	39
α-helix	336-338	3
β-strand	339-349	11	39
β-strand	355-359	5	39
β-strand	360-361	2	40
β-strand	364-365	2	40
β-strand	370	1	41
β-strand	379	1	41
β-strand	382-384	3	39
α-helix	386-388	3
β-strand	390-400	11	39
α-helix	403-405	3
α-helix	411-413	3
β-strand	414-420	7	39
α-helix	421-423	3
β-strand	432-442	11	39
β-strand	445	1	42
β-strand	453-454	2	42
β-strand	456	1	43
α-helix	457	1
β-strand	458-468	11	39
α-helix	503-514	12
α-helix	518-526	9
α-helix	534-545	12
β-strand	550	1	44
β-strand	558	1	44
β-strand	564-568	5	45
α-helix	575-584	10
β-strand	590-591	2	45
β-strand	596	1	46
α-helix	598-602	5
β-strand	603-608	6	47
β-strand	613-618	6	47
α-helix	620-623	4
β-strand	628-631	4	45
α-helix	639-651	13
β-strand	653-658	6	48
β-strand	661-666	6	48
β-strand	670-675	6	45
α-helix	688-691	4
α-helix	696-701	6
β-strand	704-708	5	45
α-helix	716-725	10
α-helix	744-756	13
β-strand	761-762	2	49
α-helix	764-779	16
α-helix	795-811	17
β-strand	816-817	2	49
α-helix	819-833	15

Chain 5: 31 helices, 22 β-strands

Element	Residues	Length	Sheet
α-helix	23-37	15
α-helix	45-55	11
β-strand	60-64	5	50
α-helix	65-71	7
α-helix	73-81	9
α-helix	83-102	20
α-helix	133-135	3
β-strand	136-140	5	50
α-helix	143-147	5
α-helix	148-150	3
α-helix	153-155	3
β-strand	159-169	11	51
β-strand	173-183	11	51
β-strand	189-193	5	51
β-strand	241-259	19	51
α-helix	266	1
β-strand	273-279	7	51
α-helix	280-282	3
β-strand	291-301	11	51
β-strand	323	1	16
β-strand	325-335	11	51
α-helix	352-361	10
α-helix	366-373	8
α-helix	382-393	12
α-helix	405-407	3
β-strand	412-415	4	52
α-helix	422-432	11
β-strand	436-440	5	52
β-strand	451-456	6	53
β-strand	461-466	6	53
α-helix	468-471	4
β-strand	476-480	5	52
α-helix	487-499	13
β-strand	518-523	6	52
α-helix	544-549	6
β-strand	552-554	3	52
α-helix	562-577	16
α-helix	596-609	14
β-strand	613-614	2	54
α-helix	616-636	21
α-helix	650-656	7
α-helix	660-666	7
β-strand	671-672	2	54
α-helix	674-684	11
α-helix	685-689	5
α-helix	690-693	4
α-helix	707-721	15
β-strand	727-729	3	55
α-helix	730-734	5
α-helix	735-739	5
α-helix	746-757	12
β-strand	761-764	4	55
β-strand	769-772	4	55

Chain 6: 37 helices, 36 β-strands

Element	Residues	Length	Sheet
β-strand	94-97	4	56
α-helix	99-101	3
α-helix	103-104	2
α-helix	106-121	16
β-strand	124-125	2	57
β-strand	132-133	2	57
α-helix	135-146	12
β-strand	150-154	5	58
α-helix	155-160	6
α-helix	161-164	4
α-helix	165-172	8
α-helix	174-192	19
α-helix	194-197	4
β-strand	198	1	59
α-helix	259	1
β-strand	260	1	59
α-helix	261	1
β-strand	264-268	5	58
β-strand	273-274	2	18
α-helix	276-278	3
α-helix	281-283	3
β-strand	287-297	11	18
α-helix	298-300	3
β-strand	301-311	11	18
β-strand	318-322	5	18
α-helix	323	1
β-strand	344-346	3	18
β-strand	352-362	11	18
α-helix	369	1
α-helix	373-375	3
β-strand	376-382	7	18
α-helix	383-385	3
β-strand	394-405	12	18
α-helix	407-410	4
β-strand	437	1	60
β-strand	438	1	40
β-strand	448	1	60
β-strand	450-461	12	18
α-helix	501-507	7
α-helix	510-521	12
α-helix	525-532	8
α-helix	541-552	12
β-strand	557-558	2	61
α-helix	563	1
β-strand	564-565	2	61
α-helix	566	1
β-strand	571-575	5	62
α-helix	582-589	8
β-strand	595-598	4	62
β-strand	604	1	63
β-strand	607	1	63
β-strand	611-614	4	64
α-helix	615	1
β-strand	621-624	4	64
α-helix	627-630	4
β-strand	635-638	4	62
α-helix	646-649	4
α-helix	654-657	4
β-strand	660-663	4	65
β-strand	664	1	21
β-strand	670-673	4	65
β-strand	677-682	6	62
α-helix	695-698	4
α-helix	703-708	6
β-strand	711-715	5	62
α-helix	721-737	17
α-helix	748-758	11
β-strand	765	1	66
α-helix	767-784	18
β-strand	791	1	24
α-helix	797-813	17
β-strand	819	1	66
α-helix	821-834	14

Chain 7: 30 helices, 34 β-strands

Element	Residues	Length	Sheet
β-strand	7-8	2	27
α-helix	14-27	14
α-helix	62-71	10
β-strand	78-81	4	67
α-helix	82-95	14
α-helix	102-108	7
α-helix	113-123	11
α-helix	125-128	4
α-helix	131-132	2
α-helix	138-155	18
α-helix	194-197	4
β-strand	202-205	4	67
α-helix	209-214	6
β-strand	224-225	2	43
α-helix	232-234	3
β-strand	238-247	10	43
β-strand	252-262	11	43
β-strand	268-272	5	43
β-strand	277-278	2	42
β-strand	283	1	68
α-helix	287-291	5
α-helix	296	1
β-strand	297	1	68
β-strand	298-300	3	43
β-strand	306-316	11	43
α-helix	323	1
β-strand	330-336	7	43
β-strand	347-357	11	43
α-helix	363-368	6
β-strand	371	1	27
β-strand	374-383	10	43
α-helix	410-413	4
α-helix	426-437	12
β-strand	444	1	69
β-strand	449	1	69
β-strand	456-460	5	70
β-strand	462	1	71
β-strand	464	1	71
α-helix	467-476	10
β-strand	480-484	5	70
β-strand	488	1	72
β-strand	491	1	72
β-strand	495-499	5	73
β-strand	506-510	5	73
α-helix	512-515	4
β-strand	520-524	5	70
α-helix	526-528	3
α-helix	531-542	12
β-strand	546-547	2	74
β-strand	549	1	46
β-strand	556-557	2	74
β-strand	562-566	5	70
α-helix	580-582	3
α-helix	588-593	6
β-strand	596-600	5	70
α-helix	606-622	17
α-helix	631-634	4
α-helix	635-647	13
β-strand	651	1	75
α-helix	654-674	21
α-helix	686-702	17
β-strand	707	1	75
α-helix	710-724	15
α-helix	725-727	3

Chain A: 8 helices, 5 β-strands

Element	Residues	Length	Sheet
α-helix	4-22	19
α-helix	29-31	3
α-helix	35-59	25
α-helix	65-103	39
α-helix	120-122	3
α-helix	125-142	18
α-helix	157-158	2
β-strand	162-167	6	1
β-strand	173-175	3	2
β-strand	180-182	3	2
β-strand	188-192	5	1
α-helix	197-202	6
β-strand	205-206	2	1

Chain B: 8 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	13-20	8
β-strand	24-29	6	3
α-helix	58-61	4
β-strand	69-72	4	3
α-helix	73-82	10
β-strand	85-87	3	3
α-helix	95-107	13
α-helix	121-129	9
α-helix	138-159	22
α-helix	160-162	3
β-strand	168-169	2	4
α-helix	175-199	25

5 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
DNA replication complex GINS protein PSF1	A	protein	208	Saccharomyces cerevisiae W303	Q12488 (AlphaFold model)
DNA replication complex GINS protein PSF2	B	protein	213	Saccharomyces cerevisiae W303	P40359 (AlphaFold model)
DNA replication complex GINS protein PSF3	C	protein	217	Saccharomyces cerevisiae W303	Q12146 (AlphaFold model)
DNA replication complex GINS protein SLD5	D	protein	294	Saccharomyces cerevisiae W303	Q03406 (AlphaFold model)
Cell division control protein 45	E	protein	650	Saccharomyces cerevisiae W303	Q08032
Leading strand DNA template	F	DNA	48	synthetic construct
Lagging strand DNA template	G	DNA	20	synthetic construct
DNA replication licensing factor MCM2	2	protein	868	Saccharomyces cerevisiae W303	P29469
DNA replication licensing factor MCM3	3	protein	971	Saccharomyces cerevisiae W303	P24279
DNA replication licensing factor MCM4	4	protein	933	Saccharomyces cerevisiae W303	P30665
Minichromosome maintenance protein 5	5	protein	775	Saccharomyces cerevisiae W303	P29496
DNA replication licensing factor MCM6	6	protein	1017	Saccharomyces cerevisiae W303	P53091

3 more molecules are not listed.

Sequence of entity 1 (A), FASTA

>9E2X_1 DNA replication complex GINS protein PSF1 (chains A)
MYGDLGNKLVLEAKRTKQLYARSNQDVNLPMYHEDIIRNILKEVSNLRKNTEYLKEQQQL
GMLDDKVAKCQYFVTLLCMERNKRCLLAYQRLRTDILDSMAWNNNGLDLMSSITFSQQDT
NNLSHQEQEYLKEYCDLITDLKSGDLVDIDLSGSLVPPSDVFIDVRVLKDAGEIQTEYGV
FNLIKDSQFFVRQSDVERLIQQGYLQKI

Sequence of entity 2 (B), FASTA

>9E2X_2 DNA replication complex GINS protein PSF2 (chains B)
MSLPAHLQQTFSPEEIQFIVENEPIKIFPRITTRQKIRGDDRGTGNHTRWQLITTDDKAL
NNMVAMRSTEVVLWIALLLKQQSKCSIVAPQWLTTKELDRKIQYEKTHPDRFSELPWNWL
VLARILFNKAKDDFHDPIHELRGKIQDLREIRQIKVLKGLKYLNESHLQLDNLSLLEINE
LRPFITEIMDKLREIHTASLTAGTENDEEEFNI

Sequence of entity 3 (C), FASTA

>9E2X_3 DNA replication complex GINS protein PSF3 (chains C)
MGSSHHHHHHSSGLVPRGSHMASMGYYDIDDVLADGTEFPCKFQYDIPGLGYLENNPGRP
ITKNTKLSLPLWLARILAIVGGDEALVDEEPVPFVELLPPDMFSTKVMNAIKTDPVALDL
HSINSHFFSLAIKWIMLFSEKELANVVSELLLQRAQELNHHASSLSIDLNADSTGKNSAN
TNIATSTFLLKLEEMEKEIYKKSHESYKDTKRWMFKK

Sequence of entity 4 (D), FASTA

>9E2X_4 DNA replication complex GINS protein SLD5 (chains D)
MDINIDDILAELDKETTAVDSTKITQGSSSTTHRDANTIVGSSLDLNDKTQIYVSPQQDF
SDLMKSWKNERCSPELLPYPHQLMKRLLNRISMQSQLIENISMGFLDMQNASNANPPMPN
ESKLPLLCMETELERLKFVIRSYIRCRLSKIDKFSLYLRQLNEDENSLISLTDLLSKDEI
KYHDTHSLIWLKLVNDSILKYMPEELQAINDTEGSVNMIDEPDWNKFVFIHVNGPPDGKW
NEDPLLQENEFGKPCYTVTIPDLKEEVELTIGSIYVMRYEVIRDLLRDDKVALI

Sequence of entity 5 (E), FASTA

>9E2X_5 Cell division control protein 45 (chains E)
MYYGISQFSEAYNKILRNSSSHSSCQLVIFVSCLNIDALCATKMLSLLFKKQLVQSQIVP
IFGYSELRRHYSQLDDNINSLLLVGFGGVIDLEAFLEIDPQEYVIDTDEKSGEQSFRRDI
YVLDAHRPWNLDNIFGSQIIQCFDDGTVDDTLGEQKEAYYKLLELDEESGDDELSGDEND
NNGGDDEATDADEVTDEDEEDEDETISNKRGNSSIGPNDLSKRKQRKKQIHEYEGVLEEY
YSQGTTVVNSISAQIYSLLSAIGETNLSNLWLNILGTTSLDIAYAQVYNRLYPLLQDEVK
RLTPSSRNSVKTPDTLTLNIQPDYYLFLLRHSSLYDSFYYSNYVNAKLSLWNENGKKRLH
KMFARMGIPLSTAQETWLYMDHSIKRELGIIFDKNLDRYGLQDIIRDGFVRTLGYRGSIS
ASEFVEALTALLEVGNSTDKDSVKINNDNNDDTDGEEEEDNSAQKLTNLRKRWVSNFWLS
WDALDDRKVELLNRGIQLAQDLQRAIFNTGVAILEKKLIKHLRIYRLCVLQDGPDLDLYR
NPLTLLRLGNWLIECCAESEDKQLLPMVLASIDENTDTYLVAGLTPRYPRGLDTIHTKKP
ILNNFSMAFQQITAETDAKVRIDNFESSIIEIRREDLSPFLEKLTLSGLL

Sequence of entity 6 (F), FASTA

>9E2X_6 Leading strand DNA template (chains F)
TCGTGCTGAGTGATATCTGCTTTGGGTGGGTGGGTGGGTTGAGGCAAT

Sequence of entity 7 (G), FASTA

>9E2X_7 Lagging strand DNA template (chains G)
GCAGATATCACTCAGCACGA

Sequence of entity 8 (2), FASTA

>9E2X_8 DNA replication licensing factor MCM2 (chains 2)
MSDNRRRRREEDDSDSENELPPSSPQQHFRGGMNPVSSPIGSPDMINPEGDDNEVDDVPD
IDEVEEQMNEVDLMDDNMYEDYAADHNRDRYDPDQVDDREQQELSLSERRRIDAQLNERD
RLLRNVAYIDDEDEEQEGAAQLDEMGLPVQRRRRRRQYEDLENSDDDLLSDMDIDPLREE
LTLESLSNVKANSYSEWITQPNVSRTIARELKSFLLEYTDETGRSVYGARIRTLGEMNSE
SLEVNYRHLAESKAILALFLAKCPEEMLKIFDLVAMEATELHYPDYARIHSEIHVRISDF
PTIYSLRELRESNLSSLVRVTGVVTRRTGVFPQLKYVKFNCLKCGSILGPFFQDSNEEIR
ISFCTNCKSKGPFRVNGEKTVYRNYQRVTLQEAPGTVPPGRLPRHREVILLADLVDVSKP
GEEVEVTGIYKNNYDGNLNAKNGFPVFATIIEANSIKRREGNTANEGEEGLDVFSWTEEE
EREFRKISRDRGIIDKIISSMAPSIYGHRDIKTAVACSLFGGVPKNVNGKHSIRGDINVL
LLGDPGTAKSQILKYVEKTAHRAVFATGQGASAVGLTASVRKDPITKEWTLEGGALVLAD
KGVCLIDEFDKMNDQDRTSIHEAMEQQSISISKAGIVTTLQARCSIIAAANPNGGRYNST
LPLAQNVSLTEPILSRFDILCVVRDLVDEEADERLATFVVDSHVRSHPENDEDREGEELK
NNGESAIEQGEDEINEQLNARQRRLQRQRKKEEEISPIPQELLMKYIHYARTKIYPKLHQ
MDMDKVSRVYADLRRESISTGSFPITVRHLESILRIAESFAKMRLSEFVSSYDLDRAIKV
VVDSFVDAQKVSVRRQLRRSFAIYTLGH

Sequence of entity 9 (3), FASTA

>9E2X_9 DNA replication licensing factor MCM3 (chains 3)
MEGSTGFDGDATTFFAPDAVFGDRVRRFQEFLDTFTSYRDSVRSIQVYNSNNAANYNDDQ
DDADERDLLGDDDGDDLEKEKKAASSTSLNILPHRIIISLDDLREFDRSFWSGILVEPAY
FIPPAEKALTDLADSMDDVPHPNASAVSSRHPWKLSFKGSFGAHALSPRTLTAQHLNKLV
SVEGIVTKTSLVRPKLIRSVHYAAKTGRFHYRDYTDATTTLTTRIPTPAIYPTEDTEGNK
LTTEYGYSTFIDHQRITVQEMPEMAPAGQLPRSIDVILDDDLVDKTKPGDRVNVVGVFKS
LGAGGMNQSNSNTLIGFKTLILGNTVYPLHARSTGVAARQMLTDFDIRNINKLSKKKDIF
DILSQSLAPSIYGHDHIKKAILLMLMGGVEKNLENGSHLRGDINILMVGDPSTAKSQLLR
FVLNTASLAIATTGRGSSGVGLTAAVTTDRETGERRLEAGAMVLADRGVVCIDEFDKMTD
VDRVAIHEVMEQQTVTIAKAGIHTTLNARCSVIAAANPVFGQYDVNRDPHQNIALPDSLL
SRFDLLFVVTDDINEIRDRSISEHVLRTHRYLPPGYLEGEPVRERLNLSLAVGEDADINP
EEHSNSGAGVENEGEDDEDHVFEKFNPLLQAGAKLAKNKGNYNGTEIPKLVTIPFLRKYV
QYAKERVIPQLTQEAINVIVKNYTDLRNDDNTKKSPITARTLETLIRLATAHAKVRLSKT
VNKVDAKVAANLLRFALLGEDIGNDIDEEESEYEEALSKRSPQKSPKKRQRVRQPASNSG
SPIKSTPRRSTASSVNATPSSARRILRFQDDEQNAGEDDNDIMSPLPADEEAELQRRLQL
GLRVSPRRREHLHAPEEGSSGPLTEVGTPRLPNVSSAGQDDEQQQSVISFDNVEPGTIST
GRLSLISGIIARLMQTEIFEEESYPVASLFERINEELPEEEKFSAQEYLAGLKIMSDRNN
LMVADDKVWRV

Sequence of entity 10 (4), FASTA

>9E2X_10 DNA replication licensing factor MCM4 (chains 4)
MSQQSSSPTKEDNNSSSPVVPNPDSVPPQLSSPALFYSSSSSQGDIYGRNNSQNLSQGEG
NIRAAIGSSPLNFPSSSQRQNSDVFQSQGRQGRIRSSASASGRSRYHSDLRSDRALPTSS
SSLGRNGQNRVHMRRNDIHTSDLSSPRRIVDFDTRSGVNTLDTSSSSAPPSEASEPLRII
WGTNVSIQECTTNFRNFLMSFKYKFRKILDEREEFINNTTDEELYYIKQLNEMRELGTSN
LNLDARNLLAYKQTEDLYHQLLNYPQEVISIMDQTIKDCMVSLIVDNNLDYDLDEIETKF
YKVRPYNVGSCKGMRELNPNDIDKLINLKGLVLRSTPVIPDMKVAFFKCNVCDHTMAVEI
DRGVIQEPARCERIDCNEPNSMSLIHNRCSFADKQVIKLQETPDFVPDGQTPHSISLCVY
DELVDSCRAGDRIEVTGTFRSIPIRANSRQRVLKSLYKTYVDVVHVKKVSDKRLDVDTST
IEQELMQNKVDHNEVEEVRQITDQDLAKIREVAAREDLYSLLARSIAPSIYELEDVKKGI
LLQLFGGTNKTFTKGGRYRGDINILLCGDPSTSKSQILQYVHKITPRGVYTSGKGSSAVG
LTAYITRDVDTKQLVLESGALVLSDGGVCCIDEFDKMSDSTRSVLHEVMEQQTISIAKAG
IITTLNARSSILASANPIGSRYNPNLPVTENIDLPPPLLSRFDLVYLVLDKVDEKNDREL
AKHLTNLYLEDKPEHISQDDVLPVEFLTMYISYAKEHIHPIITEAAKTELVRAYVGMRKM
GDDSRSDEKRITATTRQLESMIRLAEAHAKMKLKNVVELEDVQEAVRLIRSAIKDYATDP
KTGKIDMNLVQTGKSVIQRKLQEDLSREIMNVLKDQASDSMSFNELIKQINEHSQDRVES
SDIQEALSRLQQEDKVIVLGEGVRRSVRLNNRV

Sequence of entity 11 (5), FASTA

>9E2X_11 Minichromosome maintenance protein 5 (chains 5)
MSFDRPEIYSAPVLQGESPNDDDNTEIIKSFKNFILEFRLDSQFIYRDQLRNNILVKNYS
LTVNMEHLIGYNEDIYKKLSDEPSDIIPLFETAITQVAKRISILSRAQSANNNDKDPENT
SMDTDSLLLNSLPTFQLILNSNANQIPLRDLDSEHVSKIVRLSGIIISTSVLSSRATYLS
IMCRNCRHTTSITINNFNSITGNTVSLPRSCLSTIESESSMANESNIGDESTKKNCGPDP
YIIIHESSKFIDQQFLKLQEIPELVPVGEMPRNLTMTCDRYLTNKVIPGTRVTIVGIYSI
YNSKNGAGSGRSGGGNGGSGVAIRTPYIKILGIQSDVETSSIWNSVTMFTEEEEEEFLQL
SRNPKLYEILTNSIAPSIFGNEDIKKAIVCLLMGGSKKILPDGMRLRGDINVLLLGDPGT
AKSQLLKFVEKVSPIAVYTSGKGSSAAGLTASVQRDPMTREFYLEGGAMVLADGGVVCID
EFDKMRDEDRVAIHEAMEQQTISIAKAGITTVLNSRTSVLAAANPIYGRYDDLKSPGDNI
DFQTTILSRFDMIFIVKDDHNEERDISIANHVINIHTGNANAMQNQQEENGSEISIEKMK
RYITYCRLKCAPRLSPQAAEKLSSNFVTIRKQLLINELESTERSSIPITIRQLEAIIRIT
ESLAKLELSPIAQERHVDEAIRLFQASTMDAASQDPIGGLNQASGTSLSEIRRFEQELKR
RLPIGWSTSYQTLRREFVDTHRFSQLALDKALYALEKHETIQLRHQGQNIYRSGV

Sequence of entity 12 (6), FASTA

>9E2X_12 DNA replication licensing factor MCM6 (chains 6)
MSSPFPADTPSSNRPSNSSPPPSSIGAGFGSSSGLDSQIGSRLHFPSSSQPHVSNSQTGP
FVNDSTQFSSQRLQTDGSATNDMEGNEPARSFKSRALNHVKKVDDVTGEKVREAFEQFLE
DFSVQSTDTGEVEKVYRAQIEFMKIYDLNTIYIDYQHLSMRENGALAMAISEQYYRFLPF
LQKGLRRVVRKYAPELLNTSDSLKRSEGDEGQADEDEQQDDDMNGSSLPRDSGSSAAPGN
GTSAMATRSITTSTSPEQTERVFQISFFNLPTVHRIRDIRSEKIGSLLSISGTVTRTSEV
RPELYKASFTCDMCRAIVDNVEQSFKYTEPTFCPNPSCENRAFWTLNVTRSRFLDWQKVR
IQENANEIPTGSMPRTLDVILRGDSVERAKPGDRCKFTGVEIVVPDVTQLGLPGVKPSST
LDTRGISKTTEGLNSGVTGLRSLGVRDLTYKISFLACHVISIGSNIGASSPDANSNNRET
ELQMAANLQANNVYQDNERDQEVFLNSLSSDEINELKEMVKDEHIYDKLVRSIAPAVFGH
EAVKKGILLQMLGGVHKSTVEGIKLRGDINICVVGDPSTSKSQFLKYVVGFAPRSVYTSG
KASSAAGLTAAVVRDEEGGDYTIEAGALMLADNGICCIDEFDKMDISDQVAIHEAMEQQT
ISIAKAGIHATLNARTSILAAANPVGGRYNRKLSLRGNLNMTAPIMSRFDLFFVILDDCN
EKIDTELASHIVDLHMKRDEAIEPPFSAEQLRRYIKYARTFKPILTKEARSYLVEKYKEL
RKDDAQGFSRSSYRITVRQLESMIRLSEAIARANCVDEITPSFIAEAYDLLRQSIIRVDV
DDVEMDEEFDNIESQSHAASGNNDDNDDGTGSGVITSEPPADIEEGQSEATARPGTSEKK
KTTVTYDKYVSMMNMIVRKIAEVDREGAEELTAVDIVDWYLLQKENDLGSLAEYWEERRL
AFKVIKRLVKDRILMEIHGTRHNLRDLENEENENNKTVYVIHPNCEVLDQLEPQDSS

Ligands and cofactors

ID	Name	Formula	Copies
ATP	Adenosine-5'-triphosphate	C10 H16 N5 O13 P3	4
MG	Magnesium ion	Mg	5
ZN	Zinc ion	Zn	5
ADP	Adenosine-5'-diphosphate	C10 H15 N5 O10 P2	2

Primary citation

G-quadruplex-stalled eukaryotic replisome structure reveals helical inchworm DNA translocation. Batra, S., Allwein, B., Kumar, C. et al. Science (2025) 387:eadt1978-eadt1978. DOI 10.1126/science.adt1978 · PubMed

Other PDB entries of the same protein (UniProt Q12488 (AlphaFold model), which also has an AlphaFold model), best resolution first:

28VY 2.7 Å, sCMGE assembled on ARS1 DNA with Sld2 and RPA
8KG6 3.07 Å, Yeast replisome in state I
7PMK 3.2 Å, S. cerevisiae replisome-SCF(Dia2) complex bound to double-stranded DNA (conformation I)
7PMN 3.2 Å, S. cerevisiae replisome-SCF(Dia2) complex bound to double-stranded DNA (conformation II)
9E2Y 3.2 Å, Cryo-EM structure of yeast CMG helicase stalled at G4-containing DNA template, state 3
9RHI 3.2 Å, pre-Initiation Complex on ARS1 DNA (monomer)
9E2W 3.3 Å, Cryo-EM structure of yeast CMG helicase stalled at G4-containing DNA template, state 1
9RHJ 3.4 Å, Pre-Initiation Complex on ARS1 DNA (dimer)
8B9A 3.5 Å, S. cerevisiae replisome + Ctf4, bound by pol alpha primase. Complex engaged with a fork…
8B9B 3.5 Å, S. cerevisiae replisome + Ctf4, bound by pol alpha. Complex engaged with a fork DNA…
3JC6 3.7 Å, Structure of the eukaryotic replicative CMG helicase and pumpjack motion
6SKL 3.7 Å, Cryo-EM structure of the CMG Fork Protection Complex at a replication fork -…

Browse structure collections

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