Structure-specific endonuclease subunit EME1 (EME1) is a 570-residue protein from Homo sapiens. This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: Q96AY2.
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The mean pLDDT of this model is 67.5 (low overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 38% |
| 70 to 90 | Confident: backbone generally right | 14% |
| 50 to 70 | Low: treat with caution | 8% |
| Below 50 | Very low: often disordered regions | 40% |
What pLDDT means and how to read it
Non-catalytic subunit of the structure-specific, heterodimeric DNA endonuclease MUS81-EME1 which is involved in the maintenance of genome stability. In the complex, EME1 is required for DNA cleavage, participating in DNA recognition and bending (PubMed:12686547, PubMed:12721304, PubMed:14617801, PubMed:17289582, PubMed:24733841, PubMed:24813886, PubMed:35290797, PubMed:39015284). MUS81-EME1 cleaves 3'-flaps and nicked Holliday junctions, and exhibit limited endonuclease activity with 5' flaps and nicked double-stranded DNAs (PubMed:24733841, PubMed:35290797). Active during prometaphase, MUS81-EME1 resolves mitotic recombination intermediates, including Holliday junctions, which form during…
Part of the heterodimeric MUS81-EME1 complex
Nucleus, nucleolus
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 9F9L | X-ray | 2.02 Å | B/D=246-570 |
| 9F98 | X-ray | 2.15 Å | B/D=246-570 |
| 9F9M | X-ray | 2.47 Å | B/D=246-570 |
| 2ZIU | X-ray | 2.7 Å | B=246-570 |
| 2ZIV | X-ray | 2.7 Å | B=249-368, B=407-570 |
| 9F9K | X-ray | 2.73 Å | B/D=246-570 |
| 2ZIW | X-ray | 2.8 Å | B=246-570 |
| 4P0P | X-ray | 2.8 Å | B=178-570 |
| 9F99 | X-ray | 2.8 Å | B/D/F/H=246-570 |
| 4P0Q | X-ray | 2.85 Å | B=178-570 |
| 9F9A | X-ray | 2.91 Å | B/D/F/H=246-570 |
| 2ZIX | X-ray | 3.5 Å | B=246-570 |
| 4P0S | X-ray | 6.0 Å | B/D/F/H=178-570 |
| 4P0R | X-ray | 6.5 Å | B/D=178-570 |
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