4JTD: Kv1.2-2.1 paddle chimera channel

Crystal structure of Kv1.2-2.1 paddle chimera channel in complex with Lys27Met mutant of Charybdotoxin. Determined by X-ray diffraction at 2.54 Å resolution. Released 12 Jun 2013.

Method

X-ray diffraction

Resolution

2.54 Å

Organisms

Rattus norvegicus, Leiurus quinquestriatus hebraeus

Chains

5

Atoms

12,082

Mol. weight

211.19 kDa

Ligands

PGW, NAP

Released

12 Jun 2013

Explore 4JTD in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

4JTD contains 89 α-helices and 41 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 23 helices, 16 β-strands

Molecules and chains

>4JTD_1 Voltage-gated potassium channel subunit beta-2 (chains A, G)
MLQFYRNLGKSGLRVSCLGLGTWVTFGGQITDEMAEHLMTLAYDNGINLFDTAEVYAAGK
AEVVLGNIIKKKGWRRSSLVITTKIFWGGKAETERGLSRKHIIEGLKASLERLQLEYVDV
VFANRPDPNTPMEETVRAMTHVINQGMAMYWGTSRWSSMEIMEAYSVARQFNLIPPICEQ
AEYHMFQREKVEVQLPELFHKIGVGAMTWSPLACGIVSGKYDSGIPPYSRASLKGYQWLK
DKILSEEGRRQQAKLKELQAIAERLGCTLPQLAIAWCLRNEGVSSVLLGASNAEQLMENI
GAIQVLPKLSSSIVHEIDSILGNKPYSKKDYRS

>4JTD_2 Potassium voltage-gated channel subfamily A member 2, Potassium voltage-gated channel subfamily B member 1 (chains B, H)
MAHHHHHHHHHHGLVPRGSMTVATGDPVDEAAALPGHPQDTYDPEADHESSERVVINISG
LRFETQLKTLAQFPETLLGDPKKRMRYFDPLRNEYFFDRNRPSFDAILYYYQSGGRLRRP
VNVPLDIFSEEIRFYELGEEAMEMFREDEGYIKEEERPLPENEFQRQVWLLFEYPESSGP
ARIIAIVSVMVILISIVSFCLETLPIFRDENEDMHGGGVTFHTYSQSTIGYQQSTSFTDP
FFIVETLCIIWFSFEFLVRFFACPSKAGFFTNIMNIIDIVAIIPYYVTIFLTESNKSVLQ
FQNVRRVVQIFRIMRILRIFKLSRHSKGLQILGQTLKASMRELGLLIFFLFIGVILFSSA
VYFAEADERDSQFPSIPDAFWWAVVSMTTVGYGDMVPTTIGGKIVGSLCAIAGVLTIALP
VPVIVSNFNYFYHRETEGEEQAQYLQVTSSPKIPSSPDLKKSRSASTISKSDYMEIQEGV
NNSNEDFREENLKTANSTLANTNYVNITKMLTDV

>4JTD_3 Potassium channel toxin alpha-KTx 1.1 (chains Y)
QFTNVSCTTSKECWSVCQRLHNTSRGMCMNKKCRCYS

Ligands and cofactors

Water and common crystallization additives (K) are not listed.

Primary citation

Structure of a pore-blocking toxin in complex with a eukaryotic voltage-dependent K(+) channel. Banerjee, A., Lee, A., Campbell, E. et al. Elife (2013) 2:e00594-e00594. DOI 10.7554/eLife.00594 · PubMed

Related structures

Other PDB entries of the same protein (UniProt P62483 (AlphaFold model), which also has an AlphaFold model), best resolution first:

• 3EAU 1.82 Å, Voltage-dependent K+ channel beta subunit in complex with cortisone
• 3EB3 2.0 Å, Voltage-dependent K+ channel beta subunit (W121A) in complex with cortisone
• 3EB4 2.0 Å, Voltage-dependent K+ channel beta subunit (I211R) in complex with cortisone
• 1EXB 2.1 Å, Structure of the cytoplasmic beta subunit-T1 assembly of voltage-dependent K channels
• 2R9R 2.4 Å, Shaker family voltage dependent potassium channel (kv1.2-kv2.1 paddle chimera channel)…
• 4JTA 2.5 Å, Crystal structure of Kv1.2-2.1 paddle chimera channel in complex with Charybdotoxin
• 4JTC 2.56 Å, Crystal structure of Kv1.2-2.1 paddle chimera channel in complex with Charybdotoxin in Cs+
• 1QRQ 2.8 Å, Structure of a voltage-dependent K+ channel beta subunit
• 2A79 2.9 Å, Mammalian Shaker Kv1.2 potassium channel- beta subunit complex
• 3LNM 2.9 Å, F233W mutant of the Kv2.1 paddle-Kv1.2 chimera channel
• 3LUT 2.9 Å, A Structural Model for the Full-length Shaker Potassium Channel Kv1.2
• 6EBL 3.0 Å, The voltage-activated Kv1.2-2.1 paddle chimera channel in lipid nanodiscs, cytosolic…

Browse structure collections

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