Structure-specific endonuclease subunit MUS81 (MUS81) is a 551-residue protein from Homo sapiens. This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: Q96NY9.
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The mean pLDDT of this model is 77.9 (confident overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 43% |
| 70 to 90 | Confident: backbone generally right | 32% |
| 50 to 70 | Low: treat with caution | 6% |
| Below 50 | Very low: often disordered regions | 19% |
What pLDDT means and how to read it
Catalytic subunit of two functionally distinct, structure-specific, heterodimeric DNA endonucleases MUS81-EME1 and MUS81-EME2 that are involved in the maintenance of genome stability (PubMed:11741546, PubMed:12374758, PubMed:12686547, PubMed:12721304, PubMed:24371268, PubMed:24733841, PubMed:24813886, PubMed:35290797, PubMed:39015284). Both endonucleases have essentially the same substrate specificity though MUS81-EME2 is more active than its MUS81-EME1 counterpart. Both cleave 3'-flaps and nicked Holliday junctions, and exhibit limited endonuclease activity with 5' flaps and nicked double-stranded DNAs (PubMed:24371268, PubMed:24733841, PubMed:35290797). MUS81-EME2 which is active during…
Part of the heterodimeric DNA structure-specific endonuclease complex MUS81-EME1 (PubMed:12686547, PubMed:12721304, PubMed:14617801, PubMed:17289582, PubMed:35290797, PubMed:39015284). Part of the heterodimeric DNA structure-specific endonuclease complex MUS81-EME2 (PubMed:17289582, PubMed:24813886, PubMed:35290797). Interacts with BLM; may stimulate the endonuclease activity of MUS81…
Nucleus, nucleolus
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 7BU5 | X-ray | 1.8 Å | A=2-99 |
| 9F9L | X-ray | 2.02 Å | A/C=246-551 |
| 9F98 | X-ray | 2.15 Å | A/C=246-551 |
| 9F9M | X-ray | 2.47 Å | A/C=246-551 |
| 9F9K | X-ray | 2.73 Å | A/C=246-551 |
| 4P0P | X-ray | 2.8 Å | A=246-551 |
| 9F99 | X-ray | 2.8 Å | A/C/E/G=246-551 |
| 4P0Q | X-ray | 2.85 Å | A=246-551 |
| 9F9A | X-ray | 2.91 Å | A/C/E/G=246-551 |
| 7F6L | X-ray | 3.2 Å | A=246-551 |
| 2ZIX | X-ray | 3.5 Å | A=246-551 |
| 4P0S | X-ray | 6.0 Å | A/C/E/G=246-551 |
| 4P0R | X-ray | 6.5 Å | A/C=246-551 |
| 2MC3 | NMR | A=126-230 | |
| 6VWB | NMR | A=2-90 |
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