6TZA: IST1 homolog

CryoEM reconstruction of ESCRT-III filament composed of IST1 NTD R16E K27E double mutant. Determined by electron microscopy at 7.2 Å resolution. Released 8 Apr 2020.

Method: Electron microscopy
Resolution: 7.2 Å
Organism: Homo sapiens
Chains: 14
Atoms: 19,432
Mol. weight: 301.65 kDa
Released: 8 Apr 2020

Explore 6TZA in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6TZA contains 168 α-helices and 0 β-strands across 14 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chains A, B, C, D, E, F, G, H, I, J, K, L, M and N: 12 helices, 0 β-strands

Element	Residues	Length
α-helix	8-45	38
α-helix	49-81	33
α-helix	83-88	6
α-helix	94-96	3
α-helix	97-110	14
α-helix	116-127	12
α-helix	136-140	5
α-helix	141-143	3
α-helix	146-151	6
α-helix	156-158	3
α-helix	159-173	15
α-helix	181-185	5

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
IST1 homolog	A, B, C, D, E, F, G, H, I, J, K, L, M, N	protein	189	Homo sapiens	P53990 (AlphaFold model)

Sequence of entity 1 (A, B, C, D, E, F, G, H, I, J, K, L, M, N), FASTA

>6TZA_1 IST1 homolog (chains A, B, C, D, E, F, G, H, I, J, K, L, M, N)
MLGSGFKAERLRVNLELVINRLKLLEEKKTELAQKARKEIADYLAAGKDERARIRVEHII
REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV
ADQLCAKYSKEYGKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEPD
SVVMAEAPP

Primary citation

Membrane constriction and thinning by sequential ESCRT-III polymerization. Nguyen, H.C., Talledge, N., McCullough, J. et al. Nat Struct Mol Biol (2020) 27:392-399. DOI 10.1038/s41594-020-0404-x · PubMed

Other PDB entries of the same protein (UniProt P53990 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7S7J 1.15 Å, Structure of Human SPASTIN-IST1 complex.
4WZX 1.39 Å, ULK3 regulates cytokinetic abscission by phosphorylating ESCRT-III proteins
4U7E 1.6 Å, The crystal structure of the complex of LIP5 NTD and IST1 MIM
4U7I 1.79 Å, Structure of the complex of Spartin MIT and IST1 MIM
3FRR 1.8 Å, Structure of human IST1(NTD) - (residues 1-189)(P21)
13GJ 2.0 Å, IST1 bound to PI(4,5)P2 containing membrane
4U7Y 2.5 Å, Structure of the complex of VPS4B MIT and IST1 MIM
13GH 2.56 Å, IST1 bound to PI(3,5)P2 containing membrane
3FRS 2.61 Å, Structure of human IST1(NTD) (residues 1-189)(p43212)
8UC6 2.7 Å, Calpain-7:IST1 Complex
8V2S 2.72 Å, CHMP1B/IST1 dsDNA bound copolymer
6E8G 2.9 Å, CryoEM reconstruction of IST1-CHMP1B copolymer filament bound to ssDNA at 2.9 Angstrom…

Browse structure collections

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