4CDG: Bloom's syndrome helicase BLM

Crystal structure of the Bloom's syndrome helicase BLM in complex with Nanobody. Determined by X-ray diffraction at 2.79 Å resolution. Released 13 Nov 2013.

Method

X-ray diffraction

Resolution

2.79 Å

Organisms

HOMO SAPIENS, LAMA GLAMA

Chains

4

Atoms

11,711

Mol. weight

186.74 kDa

Ligands

ZN, ADP

Released

13 Nov 2013

Explore 4CDG in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

4CDG contains 77 α-helices and 57 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 37 helices, 17 β-strands

Molecules and chains

>4CDG_1 BLOOM SYNDROME PROTEIN (chains A, B)
SMSRNLKHERFQSLSFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGG
GKSLCYQLPACVSPGVTVVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDSEATNIYLQL
SKKDPIIKLLYVTPEKICASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKR
MNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQVFSMSFNRHNLKYYVLPKKPKK
VAFDCLEWIRKHHPYDSGIIYCLSRRECDTMADTLQRDGLAALAYHAGLSDSARDEVQQK
WINQDGCQVICATIAFGMGIDKPDVRFVIHASLPKSVEGYYQESGRAGRDGEISHCLLFY
TYHDVTRLKRLIMMEKDGNHHTRETHFNNLYSMVHYCENITECRRIQLLAYFGENGFNPD
FCKKHPDVSCDNCCKTKDYKTRDVTDDVKSIVRFVQEHSSSQGMRNIKHVGPSGRFTMNM
LVDIFLGSKSAKIQSGIFGKGSAYSRHNAERLFKKLILDKILDEDLYINANDQAIAYVML
GNKAQTVLNGNLKVDFMETENSSSVKKQKALVAKVSQREEMVKKCLGELTEVCKSLGKVF
GVHYFNIFNTVTLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEVISVLQKYSEWTSPAE
DSSPGMSENLYFQ

>4CDG_2 NANOBODY (chains C, D)
MKYLLPTAAAGLLLLAAQPAMAQVQLQESGGGLVQAGGSLRLSCAASGIWFSINNMAWYR
QTPGKQRERIAIITSAGTTNYVDSVKGRFTISRDDAKNTMYLQMNSLIPEDTAVYYCNLV
ADYDMGFQSFWGRGTQVTVSSHHHHHH

Ligands and cofactors

Primary citation

Crystal Structure of the Bloom'S Syndrome Helicase Indicates a Role for the Hrdc Domain in Conformational Changes. Newman, J.A., Savitsky, P., Allerston, C.K. et al. Nucleic Acids Res (2015) 43:5221. DOI 10.1093/NAR/GKV373 · PubMed

Related structures

Other PDB entries of the same protein (UniProt P54132 (AlphaFold model), which also has an AlphaFold model), best resolution first:

• 7XV0 1.5 Å, Crystal structure of RPA70N-BLMp1 fusion
• 7AUC 1.53 Å, Crystal structure of an engineered helicase domain construct for human Bloom syndrome…
• 7XUW 1.8 Å, Crystal structure of RPA70N-BLMp2 fusion
• 9P3E 2.0 Å, LC3B bound to BLM peptide
• 5LUP 2.03 Å, Structures of DHBN domain of human BLM helicase
• 5MK5 2.16 Å, Structures of DHBN domain of human BLM helicase
• 4O3M 2.3 Å, Ternary complex of Bloom's syndrome helicase
• 5U6K 2.6 Å, Crystal structure of TopBP1 BRCT4/5 in complex with a BLM phosphopeptide
• 3WE2 2.7 Å, Structure of BLM RQC domain bound to a phosphate ion
• 3WE3 2.9 Å, Structure of BLM RQC domain bound to an arsenate ion
• 7AUD 2.96 Å, Structure of an engineered helicase domain construct for human Bloom syndrome protein…
• 4CGZ 3.2 Å, Crystal structure of the Bloom's syndrome helicase BLM in complex with DNA

Browse structure collections

About this viewer

MolViewer shows 4CDG directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.