7AUD: Bloom syndrome protein,Bloom syndrome protein

Structure of an engineered helicase domain construct for human Bloom syndrome protein (BLM). Determined by X-ray diffraction at 2.96 Å resolution. Released 16 Dec 2020.

Method

X-ray diffraction

Resolution

2.96 Å

Organisms

Homo sapiens, synthetic construct

Chains

12

Atoms

25,271

Mol. weight

417.98 kDa

Ligands

ZN, ADP, RY8, MG

Released

16 Dec 2020

Explore 7AUD in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7AUD contains 165 α-helices and 78 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 27 helices, 13 β-strands

Molecules and chains

>7AUD_1 Bloom syndrome protein,Bloom syndrome protein (chains A, B, C, D, E, F)
MGSAWSHPQFEKSSGLEVLFQGGPHMSRNLKHERFQSLSFPHTKEMMKIFHKKFGLHNFR
TNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTVVISPLRSLIVDQVQKLTS
LDIPATYLTGDKTDSEATNIYLQLSKKDPIIKLLYVTPEKICASNRLISTLENLYERKLL
ARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILR
PQVFSMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPYDSGIIYCLSRRECDTMADTLQ
RDGLAALAYHAGLSDSARDEVQQKWINQDGCQVICATIAFGMGIDKPDVRFVIHASLPKS
VEGYYQESGRAGRDGEISHCLLFYTYHDVTRLKRLIMMEKDGNHHTRETHFNNLYSMVHY
CENITECRRIQLLAYFGENGFNPDFCKKHPDVSCDNCCKTKGSGGSKALVAKVSQREEMV
KKCLGELTEVCKSLGKVFGVHYFNIFNTVTLKKLAESLSSDPEVLLQIDGVTEDKLEKYG
AEVISVLQKYSEWTSPAEDSSPG

>7AUD_2 DNA (5'-D(*GP*TP*AP*CP*CP*CP*GP*AP*TP*GP*TP*GP*T)-3') (chains M, N, O, P, Q, R)
CGTACCCGATGTGTT

Ligands and cofactors

Water and common crystallization additives (PG4) are not listed.

Primary citation

Uncovering an allosteric mode of action for a selective inhibitor of human Bloom syndrome protein. Chen, X., Ali, Y.I., Fisher, C.E. et al. Elife (2021) 10. DOI 10.7554/eLife.65339 · PubMed

Related structures

Other PDB entries of the same protein (UniProt P54132 (AlphaFold model), which also has an AlphaFold model), best resolution first:

• 7XV0 1.5 Å, Crystal structure of RPA70N-BLMp1 fusion
• 7AUC 1.53 Å, Crystal structure of an engineered helicase domain construct for human Bloom syndrome…
• 7XUW 1.8 Å, Crystal structure of RPA70N-BLMp2 fusion
• 9P3E 2.0 Å, LC3B bound to BLM peptide
• 5LUP 2.03 Å, Structures of DHBN domain of human BLM helicase
• 5MK5 2.16 Å, Structures of DHBN domain of human BLM helicase
• 4O3M 2.3 Å, Ternary complex of Bloom's syndrome helicase
• 5U6K 2.6 Å, Crystal structure of TopBP1 BRCT4/5 in complex with a BLM phosphopeptide
• 3WE2 2.7 Å, Structure of BLM RQC domain bound to a phosphate ion
• 4CDG 2.79 Å, Crystal structure of the Bloom's syndrome helicase BLM in complex with Nanobody
• 3WE3 2.9 Å, Structure of BLM RQC domain bound to an arsenate ion
• 4CGZ 3.2 Å, Crystal structure of the Bloom's syndrome helicase BLM in complex with DNA

Browse structure collections

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