5LUP: DHBN domain of human BLM helicase

Structures of DHBN domain of human BLM helicase. Determined by X-ray diffraction at 2.03 Å resolution. Released 1 Mar 2017.

Method: X-ray diffraction
Resolution: 2.03 Å
Organism: Homo sapiens
Chains: 12
Atoms: 5,543
Mol. weight: 76.97 kDa
Ligands: PO4
Released: 1 Mar 2017

Explore 5LUP in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

5LUP contains 36 α-helices and 0 β-strands across 12 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	365-383	19
α-helix	388-391	4
α-helix	397-411	15

Chain B: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	363-383	21
α-helix	388-391	4
α-helix	397-410	14

Chains C and F: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	363-383	21
α-helix	388-391	4
α-helix	399-411	13

Chain D: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	365-383	19
α-helix	388-391	4
α-helix	397-410	14

Chain E: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	363-383	21
α-helix	388-391	4
α-helix	397-413	17

Chain G: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	365-385	21
α-helix	388-392	5
α-helix	397-411	15

Chain H: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	363-384	22
α-helix	388-391	4
α-helix	397-412	16

Chain I: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	364-383	20
α-helix	388-391	4
α-helix	399-411	13

3 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
BLM protein	A, B, C, D, E, F, G, I, J, K, L	protein	54	Homo sapiens	P54132 (AlphaFold model)
BLM protein	H	protein	54	Homo sapiens	P54132 (AlphaFold model)

Sequence of entity 1 (A, B, C, D, E, F, G, I, J, K, L), FASTA

>5LUP_1 BLM protein (chains A, B, C, D, E, F, G, I, J, K, L)
MDARQISLQQQLIHVMEHICKLIDTIPDDKLKLLDCGNELLQQRNIRRKLLTEV

Sequence of entity 2 (H), FASTA

>5LUP_2 BLM protein (chains H)
MDARQLSLQQQLIHVMEHICKLIDTIPDDKLKLLDCGNELLQQRNIRRKLLTEV

Ligands and cofactors

ID	Name	Formula	Copies
PO4	Phosphate ion	O4 P	2

Water and common crystallization additives (K) are not listed.

Primary citation

A helical bundle in the N-terminal domain of the BLM helicase mediates dimer and potentially hexamer formation. Shi, J., Chen, W.F., Zhang, B. et al. J Biol Chem (2017) 292:5909-5920. DOI 10.1074/jbc.M116.761510 · PubMed

Other PDB entries of the same protein (UniProt P54132 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7XV0 1.5 Å, Crystal structure of RPA70N-BLMp1 fusion
7AUC 1.53 Å, Crystal structure of an engineered helicase domain construct for human Bloom syndrome…
7XUW 1.8 Å, Crystal structure of RPA70N-BLMp2 fusion
9P3E 2.0 Å, LC3B bound to BLM peptide
5MK5 2.16 Å, Structures of DHBN domain of human BLM helicase
4O3M 2.3 Å, Ternary complex of Bloom's syndrome helicase
5U6K 2.6 Å, Crystal structure of TopBP1 BRCT4/5 in complex with a BLM phosphopeptide
3WE2 2.7 Å, Structure of BLM RQC domain bound to a phosphate ion
4CDG 2.79 Å, Crystal structure of the Bloom's syndrome helicase BLM in complex with Nanobody
3WE3 2.9 Å, Structure of BLM RQC domain bound to an arsenate ion
7AUD 2.96 Å, Structure of an engineered helicase domain construct for human Bloom syndrome protein…
4CGZ 3.2 Å, Crystal structure of the Bloom's syndrome helicase BLM in complex with DNA

Browse structure collections

About this viewer

MolViewer shows 5LUP directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.